In vitro fidelity of the prototype primate foamy virus (PFV) RT compared to HIV-1 RT.

Abstract:

:We compared the in vitro fidelity of wild-type human immunodeficiency virus type-1 (HIV-1) reverse transcriptase (RT) and the prototype foamy virus (PFV) RT. Both enzymes had similar error rates for single nucleotide substitutions; however, PFV RT did not appear to make errors at specific hotspots, like HIV-1 RT. In addition, PFV RT made more deletions and insertions than HIV-1 RT. Although the majority of the missense errors made by HIV-1 RT and PFV RT are different, relatively few of the mutations caused by either enzyme can be explained by a misalignment/slippage mechanism. We suggest that the higher polymerase activity of PFV RT could contribute to the ability of the enzyme to jump to the same or a different template.

journal_name

Virology

journal_title

Virology

authors

Boyer PL,Stenbak CR,Hoberman D,Linial ML,Hughes SH

doi

10.1016/j.virol.2007.05.034

subject

Has Abstract

pub_date

2007-10-25 00:00:00

pages

253-64

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(07)00367-4

journal_volume

367

pub_type

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