Abstract:
:We compared the in vitro fidelity of wild-type human immunodeficiency virus type-1 (HIV-1) reverse transcriptase (RT) and the prototype foamy virus (PFV) RT. Both enzymes had similar error rates for single nucleotide substitutions; however, PFV RT did not appear to make errors at specific hotspots, like HIV-1 RT. In addition, PFV RT made more deletions and insertions than HIV-1 RT. Although the majority of the missense errors made by HIV-1 RT and PFV RT are different, relatively few of the mutations caused by either enzyme can be explained by a misalignment/slippage mechanism. We suggest that the higher polymerase activity of PFV RT could contribute to the ability of the enzyme to jump to the same or a different template.
journal_name
Virologyjournal_title
Virologyauthors
Boyer PL,Stenbak CR,Hoberman D,Linial ML,Hughes SHdoi
10.1016/j.virol.2007.05.034subject
Has Abstractpub_date
2007-10-25 00:00:00pages
253-64issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(07)00367-4journal_volume
367pub_type
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