Abstract:
:Hemagglutination is an established method but has not been used previously to determine the efficacy of virus binding to a specific cellular receptor. Here we have utilized CD46-expressing erythrocytes from a transgenic mouse to establish whether and to what extent the species B adenoviruses (Ads) as well as Ad37 and Ad49 of species D can interact with CD46. A number of different agglutination patterns, and hence CD46 interactions, could be observed for the different adenovirus types. In this system Ad7p, Ad11a, and Ad14 did not agglutinate mouse erythrocytes at all. Hemagglutination of CD46 expressing erythrocytes with high efficiency was observed for the previously established CD46 users Ad11p and Ad35 as well as for the less investigated Ad34. Ad50 agglutinated with moderate efficiency. Ad16, Ad21 and Ad49 gave incomplete agglutination. Ad16 was the only adenovirus that could be eluted. No specific CD46 interaction could be observed for Ad3p or for Ad37.
journal_name
Virologyjournal_title
Virologyauthors
Andersson EK,Mei YF,Wadell Gdoi
10.1016/j.virol.2010.03.004subject
Has Abstractpub_date
2010-06-20 00:00:00pages
20-5issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(10)00156-Xjournal_volume
402pub_type
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