Abstract:
:Monoclonal antibodies (MAbs) to a recombinant form of the envelope glycoprotein gp120 of human immunodeficiency virus type 1 (HIV-1 IIIB) were raised in rats and screened for their ability to block recombinant gp120 binding to recombinant, soluble CD4 (sCD4) in vitro. Four such MAbs were identified and characterised. Each MAb bound strongly to gp120 from eight widely divergent HIV-1 strains from the United States and Africa. Two MAbs were mapped to the fourth conserved (C4) region of gp120, whereas the other two recognised an as yet undefined, conformationally sensitive epitope. MAbs to the latter epitope were the more potent in blocking the gp120-sCD4 interaction. None of the MAbs, however, had potent neutralising activity.
journal_name
Virologyjournal_title
Virologyauthors
Cordell J,Moore JP,Dean CJ,Klasse PJ,Weiss RA,McKeating JAdoi
10.1016/0042-6822(91)90755-zsubject
Has Abstractpub_date
1991-11-01 00:00:00pages
72-9issue
1eissn
0042-6822issn
1096-0341pii
0042-6822(91)90755-Zjournal_volume
185pub_type
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