Abstract:
:The assembly of the tegument of herpes simplex virus type 1 (HSV-1) is a complex process that involves a number of events at various sites within virus-infected cells. Our studies focused on determining whether tegument proteins, VP1/2 and UL37, are added to capsids located within the nucleus. Capsids were isolated from the nuclear fraction of HSV-1-infected cells and purified by rate-zonal centrifugation to separate B capsids (containing the scaffold proteins and no viral DNA) and C capsids (containing DNA and no scaffold proteins). Western blot analyses of these capsids indicated that VP1/2 associated primarily with C capsids and UL37 associated with B and C capsids. The results demonstrate that at least two of the tegument proteins of HSV-1 are associated with capsids isolated from the nuclear fraction, and these capsid-tegument protein interactions may represent initial events of the tegumentation process.
journal_name
Virologyjournal_title
Virologyauthors
Bucks MA,O'Regan KJ,Murphy MA,Wills JW,Courtney RJdoi
10.1016/j.virol.2006.11.031subject
Has Abstractpub_date
2007-05-10 00:00:00pages
316-24issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(06)00880-4journal_volume
361pub_type
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