Papillomavirus E2 protein is regulated by specific fibroblast growth factor receptors.

Abstract:

:The papillomavirus (PV) E2 protein activates transcription and replication by recruiting cellular proteins and the E1 DNA helicase to their binding sites in the viral genome. We recently demonstrated that phosphorylation of tyrosine 102 in the bovine papillomavirus (BPV-1) E2 protein restricts these activities and that fibroblast growth factor receptor-3 (FGFR3) tyrosine kinase binds PV E2. Expression of FGFR3 decreased viral replication with both wild-type and the phenylalanine substitution at position 102, inferring that another kinase targets Y102. Here we tested FGFR- 1, -2 and -4 for association with PV E2 proteins. FGFR2 but not FGFR1 or FGFR4 co-immunoprecipitated with BPV-1 E2. We found that FGFR2 suppressed replication but did not depend on phosphorylation of BPV-1 Y102. HPV-16 and -31 E2 interacted with FGFR1, -2, and -4. These results imply that the expression and activity of FGF receptors in epithelial cells can regulate the function of E2 in viral replication.

journal_name

Virology

journal_title

Virology

authors

DeSmet M,Kanginakudru S,Jose L,Xie F,Gilson T,Androphy EJ

doi

10.1016/j.virol.2018.05.013

subject

Has Abstract

pub_date

2018-08-01 00:00:00

pages

62-68

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(18)30154-5

journal_volume

521

pub_type

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