Synthesis in vitro of infectious RNA copies of the virulent satellite of turnip crinkle virus.

abstract：:Two temperature-sensitive mutants of herpes simplex virus type 1 in complementation group 1-1 were analyzed to determine if the major DNA-binding protein they produced was thermolabile. Cells infected with these mutants were analyzed for deoxyribonucleoprotein complexes containing the DNA-binding protein. These comple...

journal_title：Virology

authors： Leinbach SS,Casto JF,Pickett TK

更新日期：1984-09-01 00:00:00

abstract：:HIV-1 entry into cells requires the interaction of both HIV-1 envelope proteins and membrane lipids. We investigated the mechanism of neutralization of HIV-1 infection of primary monocyte-derived macrophages (MDM) by a murine monoclonal antibody (mAb) WR321. WR321 specifically binds phosphatidylinositol-4-phosphate an...

journal_title：Virology

authors： Jobe O,Peachman KK,Matyas GR,Asher LV,Alving CR,Rao M

更新日期：2012-09-01 00:00:00

abstract：:The mechanism of intracellular retention for the large surface protein (L) of duck hepatitis B virus (DHBV) was analyzed by examination of the transmembrane topologies and secretory properties of a collection of DHBV L mutants and compared with that of human hepatitis B virus (HBV) L. Our results demonstrate that, in ...

journal_title：Virology

authors： Gazina EV,Lin B,Gallina A,Milanesi G,Anderson DA

更新日期：1998-03-15 00:00:00

abstract：:The efficacy of combining immunization with human immunodeficiency vitus type 1 (HIV-1) DNA and HIV-1 recombinant proteins to obtain protection from chimeric simian/human immunodeficiency virus (SHIV) was determined. Four cynomolgus monkeys received four gene-gun immunizations intraepidermally of plasmid DNA encoding ...

journal_title：Virology

authors： Putkonen P,Quesada-Rolander M,Leandersson AC,Schwartz S,Thorstensson R,Okuda K,Wahren B,Hinkula J

更新日期：1998-10-25 00:00:00

abstract：:A combination of linker scanning mutagenesis and deletional analyses has been used to determine the role of individual DNA-protein binding sites on expression from the hepatitis B virus (HBV) enhancer I-X promoter (map position (mp) 1042-1354, HBV adw2). Linker scanning mutation of the EF-C site caused a 67.5% drop in...

journal_title：Virology

authors： Gustin K,Shapiro M,Lee W,Burk RD

更新日期：1993-04-01 00:00:00

abstract：:Phase I human clinical studies involving therapeutics for emerging and biodefense pathogens with low incidence, such as the severe acute respiratory syndrome coronavirus (SARS-CoV), requires at a minimum preclinical evaluation of efficacy in two well-characterized and robust animal models. Thus, a ferret SARS-CoV mode...

journal_title：Virology

authors： Chu YK,Ali GD,Jia F,Li Q,Kelvin D,Couch RC,Harrod KS,Hutt JA,Cameron C,Weiss SR,Jonsson CB

更新日期：2008-04-25 00:00:00

abstract：:An immunological relationship was detected between the coat protein of the common (U1) strain of tobacco mosaic virus (TMV) and the large subunit of the ubiquitous CO2-fixing host enzyme, ribulose-1,5-biphosphate carboxylase (RuBisCo). When assayed by Western immunoblotting or indirect ELISA, polyclonal antisera to TM...

journal_title：Virology

authors： Dietzgen RG,Zaitlin M

更新日期：1986-11-01 00:00:00

abstract：:The transcripts of the DNA of Abutilon mosaic virus, a geminivirus with a bipartite genome (DNA A and DNA B), were characterized by Northern blot hybridization, S1 nuclease assay, primer extension analysis, and sequencing of the 3' termini of cDNA clones. It was shown that transcription is bidirectional and that the t...

journal_title：Virology

authors： Frischmuth S,Frischmuth T,Jeske H

更新日期：1991-12-01 00:00:00

abstract：:Gammaherpesviruses establish life-long infection in most adults and are associated with the development of B cell lymphomas. While the interaction between gammaherpesviruses and splenic B cells has been explored, very little is known about gammaherpesvirus infection of B-1 B cells, innate-like B cells that primarily r...

journal_title：Virology

authors： Rekow MM,Darrah EJ,Mboko WP,Lange PT,Tarakanova VL

更新日期：2016-05-01 00:00:00

abstract：:Cytotoxic T-lymphocyte (CTL) epitopes within the HIV genome are subject to negative and positive selective pressures, the balance of which influences CTL escape at a given epitope. We investigated whether viral fitness requirements dictate conservation of the HLA-A2 restricted immunodominant epitope SLYNTVATL (SL9). V...

journal_title：Virology

authors： Christie NM,Willer DO,Lobritz MA,Chan JK,Arts EJ,Ostrowski MA,Cochrane A,Luscher MA,MacDonald KS

更新日期：2009-05-25 00:00:00

abstract：:The avian hematopoietic system has long been an invaluable model to study the mechanisms of cell growth and differentiation. We have developed six MAbs against either chicken embryonic hematopoietic precursor cells or retrovirus-transformed cells. MAbs Mo1, Mo2, and Mo3 recognized transformation-associated markers exp...

journal_title：Virology

authors： Liu JL,Klein PA,Moscovici MG,Moscovici C

更新日期：1992-08-01 00:00:00

abstract：:To determine the direction of the entry of DNA during in vitro bacteriophage T7 DNA packaging, incompletely packaged DNA (ipDNA) was fractionated by agarose gel electrophoresis after degradation of DNA outside of capsids and then release of packaged DNA from capsids. After fractionation, quantitative in-gel probing wi...

journal_title：Virology

authors： Son M,Watson RH,Serwer P

更新日期：1993-09-01 00:00:00

abstract：:Rotaviruses are the leading cause of severe diarrhea in infants and young children worldwide. Thus, the development of an effective rotavirus vaccine is a major public health goal. This study was performed to identify the gene or genes responsible for rotavirus virulence or host range restriction and attenuation in a ...

journal_title：Virology

authors： Hoshino Y,Saif LJ,Kang SY,Sereno MM,Chen WK,Kapikian AZ

更新日期：1995-05-10 00:00:00

abstract：:We have studied somatically murine leukemia proviral integrations in primary N-methyl-N-nitrosourea (MNU)-induced thymic lymphomas of AKR/J mice. The majority of MNU-induced lymphomas contain newly acquired murine leukemia proviral sequences. In contrast to spontaneous AKR/J lymphomas which contain multiple integratio...

journal_title：Virology

authors： Richie ER,Angel JM,McEntire B

更新日期：1988-05-01 00:00:00

abstract：:We report here the nonenzymatic self-ligation of transcripts corresponding to the peach latent mosaic viroid (PLMVd). This is the first description of this process with viroid sequences, although it has been reported to occur with human hepatitis delta virus RNA. Self-ligation occurs when the 5'-hydroxyl and the 2',3'...

journal_title：Virology

authors： Lafontaine D,Beaudry D,Marquis P,Perreault JP

更新日期：1995-10-01 00:00:00

abstract：:Equine infectious anemia virus (EIAV) is responsible of acute disease episodes characterized by fever, anemia, thrombocytopenia and anorexia in equids. The high mutation rate in EIAV genome limited the number of full genome sequences availability. In the present study, we used the SureSelect target enrichment system w...

journal_title：Virology

authors： Deshiere A,Berthet N,Lecouturier F,Gaudaire D,Hans A

更新日期：2019-11-01 00:00:00

abstract：:HIV-1 infection leads to impaired antigen-specific T cell proliferation, increased susceptibility of T cells to apoptosis, progressive impairment of T-helper 1 (Th1) responses, and altered maturation of HIV-1-specific memory cells. We have identified similar impairments in HIV-1 transgenic (Tg) rats. Tg rats developed...

journal_title：Virology

authors： Reid W,Abdelwahab S,Sadowska M,Huso D,Neal A,Ahearn A,Bryant J,Gallo RC,Lewis GK,Reitz M

更新日期：2004-03-30 00:00:00

abstract：:We report the discovery of Nylanderia fulva virus 1 (NfV-1), the first virus identified and characterized from the ant, Nylanderia fulva. The NfV-1 genome (GenBank accession KX024775) is 10,881 nucleotides in length, encoding one large open reading frame (ORF). Helicase, protease, RNA-dependent RNA polymerase, and jel...

journal_title：Virology

authors： Valles SM,Oi DH,Becnel JJ,Wetterer JK,LaPolla JS,Firth AE

更新日期：2016-09-01 00:00:00

abstract：:Hepatitis C virus (HCV) core protein is a multifunctional protein interacting with cellular and viral proteins and promoters. A tetracycline-regulated system was used to generate a HepG2 Tet-Off cell line allowing regulated expression of a full-length (191 aa) and an N(c)-truncated core protein (160 aa). In this syste...

journal_title：Virology

authors： Erhardt A,Hassan M,Heintges T,Häussinger D

更新日期：2002-01-20 00:00:00

abstract：:In HSV types 1 and 2, gH forms a noncovalent heterodimer with gL. Previous studies demonstrated that the first 323 amino acids of gH1 and the first 161 amino acids of gL1 are sufficient for gH/gL binding. For gL1, substitution of any of its four cysteine (C) residues (all located within the gH/gL binding region) destr...

journal_title：Virology

authors： Cairns TM,Landsburg DJ,Whitbeck JC,Eisenberg RJ,Cohen GH

更新日期：2005-02-20 00:00:00

abstract：:Previously, we showed that the transmembrane (M) and surface (S) glycoproteins were recognized by splenic CD4 T lymphocytes harvested from mice infected intraperitoneally with mouse hepatitis virus, strain JHM (MHV-JHM), whereas only the S protein was recognized by splenocytes derived from mice with MHV-induced chroni...

journal_title：Virology

authors： Xue S,Perlman S

更新日期：1997-11-10 00:00:00

abstract：:A full-size cloned cDNA copy of the rotavirus gene encoding the structural neutralization glycoprotein (VP7) of Nebraska calf diarrhea virus (NCDV), a strain recently shown to be effective as a vaccine in children, has been sequenced. Comparison of the deduced amino acid sequence of NCDV (serotype 6) VP7 with that of ...

journal_title：Virology

authors： Glass RI,Keith J,Nakagomi O,Nakagomi T,Askaa J,Kapikian AZ,Chanock RM,Flores J

更新日期：1985-03-01 00:00:00

abstract：:Plant virus-encoded movement protein(s) (MP), and for many viruses the coat protein (CP), is required to mediate viral spread between plant cells via plasmodesmata (PD). Most probably, the genomic RNA of potexviruses moves through PD as assembled virions and/or as ribonucleoprotein complexes containing the CP and 25-k...

journal_title：Virology

authors： Atabekov JG,Rodionova NP,Karpova OV,Kozlovsky SV,Poljakov VY

更新日期：2000-06-05 00:00:00

abstract：:The co-existence of hepatitis B surface antigen (HBsAg) and anti-HBs antibodies (HBsAb) in serum of hepatitis B virus (HBV)-chronic carriers has been previously associated with HBsAg-amino acid (aa) substitutions. However, the aa pattern of HBV-reverse transcriptase (RT) and the clinical settings associated with this ...

journal_title：Virology

authors： Colson P,Borentain P,Motte A,Henry M,Moal V,Botta-Fridlund D,Tamalet C,Gérolami R

更新日期：2007-10-10 00:00:00

abstract：:Viral metagenomics coupled to high-throughput sequencing has provided a powerful tool for large-scale detection of known and unknown viruses associated to distinct hosts and environments. Using this approach, known and novel viruses have been characterized from sylvatic and commercial avian hosts, increasing our under...

journal_title：Virology

authors： Cibulski S,Weber MN,de Sales Lima FE,Lima DA,Fernandes Dos Santos H,Teixeira TF,Varela APM,Tochetto C,Mayer FQ,Roehe PM

更新日期：2020-09-01 00:00:00

abstract：:RNA analysis by nondenaturing polyacrylamide gel electrophoresis and Northern blot hybridization of avocado chloroplasts purified from protoplasts of leaves infected by avocado sunblotch viroid (ASBVd) revealed the main ASBVd-specific bands found previously in preparations of total leaf RNA: the monomeric, dimeric and...

journal_title：Virology

authors： Navarro JA,Darós JA,Flores R

更新日期：1999-01-05 00:00:00

abstract：:Insertion of a functional splicing cassette into a construct containing the HIV-1 poly(A) site followed by the adenovirus L3 poly(A) site results in both specific stimulation of 3' end processing at the HIV-1 site and an increase in the steady-state levels of RNA processed at both sites. To further evaluate this influ...

journal_title：Virology

authors： Scott JM,Imperiale MJ

更新日期：1996-10-15 00:00:00

abstract：:The expression of type I murine interferon (MuIFN) genes and several other cellular genes was examined in poly rI.rC induced and Newcastle disease virus (NDV) infected mouse cells. Northern analysis of RNA from induced L cells revealed that the MuIFN-alpha s are expressed efficiently in NDV infected cells but only at ...

journal_title：Virology

authors： Kelley KA,Pitha PM

更新日期：1985-12-01 00:00:00

abstract：:Treatment of HeLa cells with human lymphoblastoid interferon (IFN-alpha) does not inhibit reovirus type 3 protein synthesis during virus infection. In contrast, reovirus translation is blocked by treatment of L cells with mouse IFN-alpha. The (2'-5')A synthetase activity is induced in HeLa cells by IFN-alpha treatment...

journal_title：Virology

authors： Feduchi E,Esteban M,Carrasco L

更新日期：1988-06-01 00:00:00

abstract：:Rotavirus (RV) is the leading cause of infantile gastroenteritis worldwide. RV nonstructural protein 4 (NSP4), the first characterized viral enterotoxin, is a 28-kDa glycoprotein that has pleiotropic functions in RV infection and pathogenesis. NSP4 has multiple forms enabling it to perform its different functions. Dis...

journal_title：Virology

authors： Hyser JM,Zeng CQ,Beharry Z,Palzkill T,Estes MK

更新日期：2008-03-30 00:00:00