Abstract:
:The transcripts of the DNA of Abutilon mosaic virus, a geminivirus with a bipartite genome (DNA A and DNA B), were characterized by Northern blot hybridization, S1 nuclease assay, primer extension analysis, and sequencing of the 3' termini of cDNA clones. It was shown that transcription is bidirectional and that the transcripts are polyadenylated. Two overlapping transcripts of 1.6 and 0.7 kb were mapped to the complementary strand of DNA A and two of 1.3 and 1.2 kb to the complementary strand of DNA B. One transcript of 0.9 kb was mapped to the viral sense in DNA A and one of 1.0 kb to the viral sense in DNA B. The ends of complementary and viral mRNA overlapped in both genome parts in regions rich in polyadenylation signals.
journal_name
Virologyjournal_title
Virologyauthors
Frischmuth S,Frischmuth T,Jeske Hdoi
10.1016/0042-6822(91)90530-osubject
Has Abstractpub_date
1991-12-01 00:00:00pages
596-604issue
2eissn
0042-6822issn
1096-0341pii
0042-6822(91)90530-Ojournal_volume
185pub_type
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