Abstract:
:Dendrolimus punctatus tetravirus (DpTV) belongs to the genus omegatetravirus of the Alphatetraviridae family. Sequence analysis predicts that DpTV replicase contains a putative helicase domain (Hel). However, the helicase activity in alphatetraviruses has never been formally determined. In this study, we determined that DpTV Hel is a functional RNA helicase belonging to superfamily-1 helicase with 5'-3' dsRNA unwinding directionality. Further characterization determined the length requirement of the 5' single-stranded tail on the RNA template and the optimal reaction conditions for the unwinding activity of DpTV Hel. Moreover, DpTV Hel also contains NTPase activity. The ATPase activity of DpTV Hel could be significantly stimulated by dsRNA, and dsRNA could partially rescue the ATPase activity abolishment caused by mutations. Our study is the first to identify an alphatetravirus RNA helicase and further characterize its dsRNA unwinding and NTPase activities in detail and should foster our understanding of DpTV and other alphatetraviruses.
journal_name
Virologyjournal_title
Virologyauthors
Wang Q,Han Y,Qiu Y,Zhang S,Tang F,Wang Y,Zhang J,Hu Y,Zhou Xdoi
10.1016/j.virol.2012.08.045subject
Has Abstractpub_date
2012-11-25 00:00:00pages
440-8issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(12)00426-6journal_volume
433pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The yellow fever 17D virus is attenuated and used for human vaccination. Two of its substrains, 17D-204 and 17DD, are used for vaccine production. One of the remarkable properties of this vaccine is limited viral replication in the host but with significant dissemination of the viral mass, yielding a robust and long-l...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00583-x
更新日期:2003-11-10 00:00:00
abstract::Two temperature-sensitive mutants of cowpea mosaic virus have been isolated following nitrous acid mutagenesis. One mutant, 8-14, was shown to have a temperature-sensitive defect in the B component RNA. The location of the temperature-sensitive defect of the other mutant, 8-10, was not unambiguously determined. No vir...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90258-2
更新日期:1985-03-01 00:00:00
abstract::All primates studied to date produce retroviral-like particles in their placentae. We have purified these particles from two primate species, one Old World (human) and one New World (marmoset), and have identified the retroviral sequences which are packaged into these particles. Three families of sequences have been d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0443
更新日期:1996-08-15 00:00:00
abstract::Transient expression studies using a Nicotiana benthamiana suspension cell-derived protoplast system have identified a minimal sequence that is necessary and sufficient for activation of the tomato golden mosaic virus coat protein (CP) promoter by the viral TrAP protein (also called AL2). The sequence has a bipartite ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1757
更新日期:2003-01-20 00:00:00
abstract::Magnetic birefringence measurements are reported on solutions of two double-stranded DNA containing viruses: icosahedral cauliflower mosaic virus (CaMV) and SD, an isometric bacteriophage with a short tail. Assuming the signal comes predominantly from the DNA, constraints can be put on its packing geometry. The anisot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90232-1
更新日期:1986-12-01 00:00:00
abstract::RNA analysis by nondenaturing polyacrylamide gel electrophoresis and Northern blot hybridization of avocado chloroplasts purified from protoplasts of leaves infected by avocado sunblotch viroid (ASBVd) revealed the main ASBVd-specific bands found previously in preparations of total leaf RNA: the monomeric, dimeric and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9497
更新日期:1999-01-05 00:00:00
abstract::Monoclonal antibodies have been produced and used to map the functional topography of the surface proteins of rhesus rotavirus (RRV) that mediate viral neutralization. Ten monoclonal antibodies directed to VP7 were studied in neutralization assays and competitive binding studies. A large neutralization domain with sev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90205-9
更新日期:1986-12-01 00:00:00
abstract::Whole genome sequences for three Epstein-Barr virus strains (B95-8, GD1 and AG876) were aligned and compared. In addition to known variable loci (including type-specific alleles for the EBNA2, EBNA3A, EBNA3B and EBNA3C genes, plus the EBNA1 and LMP1 genes), seven large-scale regions of lower-level diversity were ident...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.009
更新日期:2007-03-01 00:00:00
abstract::Strong evidence indicates that virions of mammalian reoviruses undergo proteolytic processing by acid-dependent cellular proteinases as an essential step in productive infection. Proteolytic processing takes the form of a series of cleavages of outer-capsid proteins final sigma3 and mu1/mu1C. Previous studies showed a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9434
更新日期:1998-11-25 00:00:00
abstract::HIV-1 envelope glycoproteins (Env) and Env-based immunogens usually do not interact efficiently with the inferred germline precursors of known broadly neutralizing antibodies (bNAbs). This deficiency may be one reason why Env and Env-based immunogens are not efficient at inducing bNAbs. We evaluated the binding of 15 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.08.002
更新日期:2015-12-01 00:00:00
abstract::The Psu (Polarity suppression) protein of satellite bacteriophage P4 was first characterized as an anti-terminator of transcription termination in Escherichia coli. Psu is also a structural component of mature P4 capsids, where it is present as a decoration protein. Psu is located externally on the capsid surface, and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1307
更新日期:1993-06-01 00:00:00
abstract::The amino-terminal domain of influenza A virus matrix protein (residues 1-164) was crystallized at pH 7 into a new crystal form in space group P1. This packing of the protein implies that M1(1-164) was monomeric in solution when it crystallized. Otherwise, the structure of the M1 fragment in the pH 7 crystals was the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0727
更新日期:2001-01-20 00:00:00
abstract::Expression of alphavirus glycoproteins on the surface of infected cells leads to cell fusion after exposure to acidic pH. Two strains of Sindbis virus, AR339 (SV) and neuroadapted Sindbis virus (NSV), which differ in virulence for weanling mice, were found to differ in pH-dependent fusion. BHK-21 cells infected with S...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90178-5
更新日期:1989-04-01 00:00:00
abstract::Chlorella virus PBCV-1 encodes two putative chitinase genes, a181/182r and a260r, and one chitosanase gene, a292l. The three genes were cloned and expressed in Escherichia coli. The recombinant A181/182R protein has endochitinase activity, recombinant A260R has both endochitinase and exochitinase activities, and recom...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9958
更新日期:1999-10-25 00:00:00
abstract::The sequences of 189 residues from the 3' terminus of three RNAs of one strain and two RNAs of another strain of tomato aspermy virus have been determined; there was almost complete sequence homology between the RNAs. A base-paired transfer RNA-like structure is proposed for tomato aspermy virus RNAs which is similar ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90639-5
更新日期:1981-07-15 00:00:00
abstract::To be incorporated into progeny virions, the viral genome must be transported to the inner leaflet of the plasma membrane (PM) and accumulate there. Some viruses utilize lipid components to assemble at the PM. For example, simian virus 40 (SV40) targets the ganglioside GM1 and human immunodeficiency virus type 1 (HIV-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.05.018
更新日期:2016-09-01 00:00:00
abstract::We have previously shown that the late promoter of the human papovavirus BK (prototype) is contained within the three 68-bp repeats and a 66-bp region to the late side of the repeats which together constitute the early promoter enhancer. We have now carried out unidirectional deletion and linker scan analyses of these...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90053-6
更新日期:1989-03-01 00:00:00
abstract::A minor core protein, VP4, of bluetongue virus serotype 10 (BTV-10) has been synthesized in insect cells infected with a genetically manipulated recombinant baculovirus. When insect cells were coinfected by this recombinant virus and a recombinant baculovirus expressing the two major core proteins (VP3 and VP7) of the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90600-t
更新日期:1992-08-01 00:00:00
abstract::High-risk human papillomaviruses (HPVs) are etiologically implicated in human cervical cancer. Two viral genes, E6 and E7, are commonly found expressed in these cancer cells. We have previously shown that mice transgenic for the HPV-16 E6 gene or E7 gene, in which the E6 or E7 was expressed in the basal layer of epith...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0106
更新日期:2000-02-15 00:00:00
abstract::Replication of the satellite RNA of tobacco ringspot virus (sTobRV RNA) has been postulated to require rolling circle transcription. The expected product of rolling circle transcription, multimeric sTobRV RNA, is known to undergo self-cleavage in vitro to release unit-length sTobRV RNA. A spontaneous, efficient, not e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90005-v
更新日期:1991-01-01 00:00:00
abstract::The retroviral integrase (IN) is a virus-encoded enzyme that is essential for insertion of viral DNA into the host chromosome. In order to map and define the properties of a minimal functional domain for this unique viral enzyme, a series of N- and C-terminal deletions of both Rous sarcoma virus (RSV) and human immuno...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80060-3
更新日期:1995-01-10 00:00:00
abstract::We compared the outcome of different cellular and viral factors on the regulation of the HPV-16 early viral gene expression in trophoblastic and cervical cancer cells. A high variability of the long control (LCR) activity was observed, prompting us to evaluate the role of secreted factors in the control of the early g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.12.056
更新日期:2011-03-30 00:00:00
abstract::The nucleotide sequences of the genomes of two strains of canine adenovirus, type 1 (Glaxo and CLL) have been determined within the region spanning approximately 0.80-0.91 map unit. Homology comparisons with other adenoviral DNAs indicate that this region contains part of the hexon-associated protein (pVIII) precursor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90142-x
更新日期:1991-07-01 00:00:00
abstract::ARGONAUTEs (notably AGO1 and AGO2) are effectors of plant antiviral RNA silencing. AGO1 was shown to be required for the temperature-dependent symptom recovery of Nicotiana benthamiana plants infected with tomato ringspot virus (isolate ToRSV-Rasp1) at 27 °C. In this study, we show that symptom recovery from isolate T...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.08.016
更新日期:2018-11-01 00:00:00
abstract::We have characterized the fibergenes of adenovirus type 19p (Ad19p), Ad19a, and Ad37 by sequencing. The fiber genes of Ad19a and Ad37 are identical and only five amino acids differ comparing Ad19a/Ad37 with Ad19p. Based on the translated sequences we calculated the isoelectrical points (Ips) and found that the fiber k...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8269
更新日期:1997-01-06 00:00:00
abstract::West Nile virus (WNV) is a mosquito-borne flavivirus that causes febrile illness, encephalitis, and occasionally death in humans. The envelope protein is the main component of the WNV virion surface, and domain III of the envelope protein (EIII) is both a putative receptor binding domain and a target of highly specifi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.05.024
更新日期:2016-09-01 00:00:00
abstract::Seven envelope regions from two series of patient isolates have been molecularly cloned and analyzed for replication phenotypes and immunogenicity. Growth potential was analyzed for env sequences substituted into an HIV-1-NL4-3 backbone (NL4-3/env recombinants). Immunogenicity studies were conducted on secreted monome...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8445
更新日期:1997-03-03 00:00:00
abstract::To define the number of rhinovirus serotypes, cross neutralization tests and characterization studies were completed on 25 candidate prototype rhinoviruses submitted to a third phase of a collaborative program. Based on the results, 11 distinct prototype strains were designated and the numbering system was extended to...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90367-9
更新日期:1987-07-01 00:00:00
abstract::Infectious bronchitis has remained one of the most difficult to control diseases in poultry since it was first described in 1931. Previous studies demonstrated that primary CD8(+) T lymphocytes collected at 10 days post-infection (p.i.) are important in controlling acute infection. To further investigate the role of m...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00059-4
更新日期:2003-02-15 00:00:00
abstract::Assembly of the viral genome into virions is a critical process of the virus life cycle often defining the ability of the virus to move within the plant and to be transmitted horizontally to other plants. Closteroviridae virions are polar helical rods assembled primarily by a major coat protein, but with a related min...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0638
更新日期:2000-12-05 00:00:00