当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > HIV-1 Env glycoproteins from two series of primary isolates: replication phenotype and immunogenicity.

HIV-1 Env glycoproteins from two series of primary isolates: replication phenotype and immunogenicity.

Abstract：

:Seven envelope regions from two series of patient isolates have been molecularly cloned and analyzed for replication phenotypes and immunogenicity. Growth potential was analyzed for env sequences substituted into an HIV-1-NL4-3 backbone (NL4-3/env recombinants). Immunogenicity studies were conducted on secreted monomeric (gp120) and oligomeric (gp140) forms of the Envs using Env-expressing plasmid DNAs for immunizations. The env regions of the patient isolates conferred a spectrum of replication kinetics and cytotropisms on the NL4-3/env recombinants. Both patient series included non-syncytium-inducing viruses with no ability to grow on T-cell lines, and highly syncytium inducing viruses which grew well on T-cell lines. These differences in growth potential did not correlate with the ability of the DNA-expressed Envs to raise antibody in rabbits. Rather, the relative immunogenicity of the Envs was patient and form specific. The Envs from patient 5 raised higher titers of antibody than the Envs from patient 6. For each primary Env, the gp120 form of the Env raised higher titers of antibody than the gp140 form. Thus, structural features of Env that affect replication do not necessarily affect the ability to raise antibody.

journal_name

Virology

journal_title

Virology

authors

Mustafa F,Richmond JF,Fernandez-Larsson R,Lu S,Fredriksson R,Fenyö EM,O'Connell M,Johnson E,Weng J,Santoro JC,Robinson HL

doi

10.1006/viro.1997.8445

subject

Has Abstract

pub_date

1997-03-03 00:00:00

pages

269-78

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(97)98445-2

journal_volume

229

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Cell entry by a novel European filovirus requires host endosomal cysteine proteases and Niemann-Pick C1.

    abstract：:Lloviu virus (LLOV), a phylogenetically divergent filovirus, is the proposed etiologic agent of die-offs of Schreibers's long-fingered bats (Miniopterus schreibersii) in western Europe. Studies of LLOV remain limited because the infectious agent has not yet been isolated. Here, we generated a recombinant vesicular sto...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.08.019

    authors： Ng M,Ndungo E,Jangra RK,Cai Y,Postnikova E,Radoshitzky SR,Dye JM,Ramírez de Arellano E,Negredo A,Palacios G,Kuhn JH,Chandran K

    更新日期：2014-11-01 00:00:00

  • Expression of SV40 receptors on apical surfaces of polarized epithelial cells.

    abstract：:We have investigated the interaction of SV40 virions with polarized monkey kidney epithelial cells. Virions were tagged with biotin to facilitate their detection and were found to retain full infectivity. When polarized Vero C1008 cells were incubated with biotinylated virions followed by a strepavidin-rhodamine conju...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)91225-j

    authors： Basak S,Turner H,Compans RW

    更新日期：1992-09-01 00:00:00

  • Rat monoclonal antibodies to nonoverlapping epitopes of human immunodeficiency virus type 1 gp120 block CD4 binding in vitro.

    abstract：:Monoclonal antibodies (MAbs) to a recombinant form of the envelope glycoprotein gp120 of human immunodeficiency virus type 1 (HIV-1 IIIB) were raised in rats and screened for their ability to block recombinant gp120 binding to recombinant, soluble CD4 (sCD4) in vitro. Four such MAbs were identified and characterised. ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90755-z

    authors： Cordell J,Moore JP,Dean CJ,Klasse PJ,Weiss RA,McKeating JA

    更新日期：1991-11-01 00:00:00

  • Human APOBEC3G incorporation into murine leukemia virus particles.

    abstract：:The human APOBEC3G protein exhibits broad antiretroviral activity against a variety of retroviruses. It is packaged into viral particles and executes its antiviral function in the target cell. The packaging of APOBEC3G into different viral particles requires a mechanism that confers this promiscuity. Here, APOBEC3G in...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.04.006

    authors： Kremer M,Bittner A,Schnierle BS

    更新日期：2005-06-20 00:00:00

  • Discovery of endogenous retroviruses with mammalian envelopes in avian genomes uncovers long-term bird-mammal interaction.

    abstract：:Endogenous retroviruses (ERVs) arise from the infection and integration of past retroviruses into animal hosts. We performed large-scale genomic mining of 101 avian genomes for discovery of ERVs having none-avian origin and investigated the cross-species transmission events. Phylogenetic analysis of the reverse transc...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.02.005

    authors： Chen M,Cui J

    更新日期：2019-04-01 00:00:00

  • Ultrastructural analysis of the interaction between F-actin and respiratory syncytial virus during virus assembly.

    abstract：:During respiratory syncytial virus (RSV) infection there is a close physical interaction between the filamentous actin (F-actin) and the virus, involving both inclusion bodies and the virus filaments. This interaction appears to occur relatively early in the replication cycle, and can be detected from 8 h post-infecti...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.08.007

    authors： Jeffree CE,Brown G,Aitken J,Su-Yin DY,Tan BH,Sugrue RJ

    更新日期：2007-12-20 00:00:00

  • Simvastatin and oseltamivir combination therapy does not improve the effectiveness of oseltamivir alone following highly pathogenic avian H5N1 influenza virus infection in mice.

    abstract：:Nonspecific anti-inflammatory drugs have been purported to reduce the burden of severe influenza disease. We demonstrate that, unlike oseltamivir administration, simvastatin administration did not reduce morbidity, mortality, or viral load of mice infected with H1N1 or H5N1 viruses. No added benefit to the efficacy of...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.01.017

    authors： Belser JA,Szretter KJ,Katz JM,Tumpey TM

    更新日期：2013-04-25 00:00:00

  • Adenovirus infection inactivates the translational inhibitors 4E-BP1 and 4E-BP2.

    abstract：:Infection with many viruses results in the selective shutoff of host protein synthesis. A common target for virus interference with host protein synthesis is the cap-binding protein complex, eIF4F. The large subunit of the complex, eIF4G, is cleaved upon picornavirus (except cardiovirus) infection. Infection with aden...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8757

    authors： Gingras AC,Sonenberg N

    更新日期：1997-10-13 00:00:00

  • Interferon-beta and interferon-gamma synergistically inhibit the replication of severe acute respiratory syndrome-associated coronavirus (SARS-CoV).

    abstract：:Recent studies have shown that interferon-gamma (IFN-gamma) synergizes with IFN-alpha/beta to inhibit the replication of both RNA and DNA viruses. We investigated the effects of IFNs on the replication of two strains of severe acute respiratory syndrome-associated coronavirus (SARS-CoV). While treatment of Vero E6 cel...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.08.011

    authors： Sainz B Jr,Mossel EC,Peters CJ,Garry RF

    更新日期：2004-11-10 00:00:00

  • Flanders virus replication and protein synthesis.

    abstract：:The prototype strain of Flanders virus (61-7484) has been adapted to grow in Vero cell cultures and a plaque assay has been developed. Electron micrographs of infected cells revealed rod-shaped virions budding in clusters from the plasma membrane, consistent with the tentative classification of Flanders virus as a mem...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90275-9

    authors： Boyd KR,Whitaker-Dowling P

    更新日期：1988-04-01 00:00:00

  • The SIVmac239 Pr55Gag isoform, SIV p43, suppresses proteolytic cleavage of Pr55Gag.

    abstract：:In human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) the gag gene encodes the precursor polyprotein Pr55Gag, which is cleaved by the viral protease to produce the major structural proteins. Recently, it has been shown that HIV and SIV gag RNAs contain internal ribosome entry sites (IR...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.10.003

    authors： Nicholson MG,Barber SA,Clements JE

    更新日期：2007-03-30 00:00:00

  • Biology of the temperate Streptococcus thermophilus bacteriophage TP-J34 and physical characterization of the phage genome.

    abstract：:The temperate Streptococcus thermophilus bacteriophage TP-J34 was identified in the lysogenic host strain J34. The majority of phage particles produced upon induction was defective and noninfectious, consisting of DNA-filled heads lacking tails. A physical map (45.6 kb) was established. Analysis of minor restriction b...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00516-6

    authors： Neve H,Freudenberg W,Diestel-Feddersen F,Ehlert R,Heller KJ

    更新日期：2003-10-10 00:00:00

  • CDK/ERK-mediated phosphorylation of the human influenza A virus NS1 protein at threonine-215.

    abstract：:Posttranslational modification of viral proteins by cellular enzymes is a feature of many virus replication strategies. Here, we report that during infection the multifunctional human influenza A virus NS1 protein is phosphorylated at threonine-215. Substitution of alanine for threonine at this position reduced early ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.10.002

    authors： Hale BG,Knebel A,Botting CH,Galloway CS,Precious BL,Jackson D,Elliott RM,Randall RE

    更新日期：2009-01-05 00:00:00

  • The distribution and kinetics of polyomavirus in lungs of intranasally infected newborn mice.

    abstract：:The primary cell types that sustain polyomavirus (Py) replication following intranasal infection as well as the nature of the host cellular response to Py were unknown. As this is an essential and specific site for virus entry, it seems likely that viral gene function must be adapted to these mucosal tissues. Using im...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.0030

    authors： Gottlieb K,Villarreal LP

    更新日期：2000-01-05 00:00:00

  • Deletion analysis of four of eighteen late gene expression factor gene homologues of the baculovirus, BmNPV.

    abstract：:Nucleotide sequence analysis of the genome of the baculovirus Bombyx mori nuclear polyhedrosis virus (BmNPV) identified 18 homologues of the Autographa californica NPV (AcNPV) lefs (late expression factor genes). These BmNPV lefs showed high (73-98%) amino acid sequence identities to AcNPV lefs and were localized to s...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8457

    authors： Gomi S,Zhou CE,Yih W,Majima K,Maeda S

    更新日期：1997-03-31 00:00:00

  • Evidence for replication of cauliflower mosaic virus DNA in plant nuclei.

    abstract：:Cellular organelles of cauliflower mosaic virus-infected turnip plants were isolated, incubated with 32P-labeled deoxyribonucleoside triphosphates and assayed for their ability to synthesize virus-specific DNA. Only nuclei were found to synthesize a labeled DNA product. This DNA migrated during agarose gel electrophor...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(82)90124-6

    authors： Ansa OA,Bowyer JW,Shepherd RJ

    更新日期：1982-08-01 00:00:00

  • Evidence that N-linked glycosylation is necessary for hepatitis B virus secretion.

    abstract：:Human hepatitis B virus (HBV) envelopes contain three distinct glycoproteins called L, M, and S HBsAg. Each is posttranslationally modified to contain N-linked oligosaccharides. N-linked oligosaccharides, after attachment to a polypeptide backbone, are processed by enzymes within the endoplasmic reticulum (ER). There ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.0038

    authors： Lu X,Mehta A,Dwek R,Butters T,Block T

    更新日期：1995-11-10 00:00:00

  • The SARS-CoV ferret model in an infection-challenge study.

    abstract：:Phase I human clinical studies involving therapeutics for emerging and biodefense pathogens with low incidence, such as the severe acute respiratory syndrome coronavirus (SARS-CoV), requires at a minimum preclinical evaluation of efficacy in two well-characterized and robust animal models. Thus, a ferret SARS-CoV mode...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.12.032

    authors： Chu YK,Ali GD,Jia F,Li Q,Kelvin D,Couch RC,Harrod KS,Hutt JA,Cameron C,Weiss SR,Jonsson CB

    更新日期：2008-04-25 00:00:00

  • Different HTLV-I neutralization patterns among sera of patients infected with cosmopolitan HTLV-I.

    abstract：:To determine if sequence variations observed in cosmopolitan HTLV-I interfered with viral recognition by neutralizing antibodies, we evaluated the neutralization potential of sera from persons infected by HTLV-I of this clade selected for amino acid changes in their eny glycoproteins. Each serum was used to neutralize...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9139

    authors： Blanchard S,Astier-Gin T,Moynet D,Edouard E,Guillemain B

    更新日期：1998-05-25 00:00:00

  • In vitro dissection of the membrane and RNP binding activities of influenza virus M1 protein.

    abstract：:Spontaneous proteolysis of influenza virus M1 protein during crystallisation has defined an N-terminal domain of amino acids 1--164. Full-length M1, the N-terminal domain, and the C-terminal part of M1 (residues 165--252) were produced in Escherichia coli. In vitro tests showed that only full-length M1 and its N-termi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0804

    authors： Baudin F,Petit I,Weissenhorn W,Ruigrok RW

    更新日期：2001-03-01 00:00:00

  • Identification of genetic determinants of a tick-borne flavivirus associated with host-specific adaptation and pathogenicity.

    abstract：:Tick-borne flaviviruses are maintained in nature in an enzootic cycle involving a tick vector and a vertebrate host. Thus, the virus replicates in two disparate hosts, each providing selective pressures that can influence virus replication and pathogenicity. To identify viral determinants associated with replication i...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.08.030

    authors： Mitzel DN,Best SM,Masnick MF,Porcella SF,Wolfinbarger JB,Bloom ME

    更新日期：2008-11-25 00:00:00

  • Reovirus type 3 synthesizes proteins in interferon-treated HeLa cells without reversing the antiviral state.

    abstract：:Treatment of HeLa cells with human lymphoblastoid interferon (IFN-alpha) does not inhibit reovirus type 3 protein synthesis during virus infection. In contrast, reovirus translation is blocked by treatment of L cells with mouse IFN-alpha. The (2'-5')A synthetase activity is induced in HeLa cells by IFN-alpha treatment...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90555-7

    authors： Feduchi E,Esteban M,Carrasco L

    更新日期：1988-06-01 00:00:00

  • Immunization with dissociated neuraminidase, matrix, and nucleoproteins from influenza A virus eliminates cognate help and antigenic competition.

    abstract：:When hemagglutinin (HA) and neuraminidase (NA) are presented together on an intact influenza virus particle, the antigens are competitive, with HA dominant over NA in T- and B-cell priming. Immunization with mixtures of purified HA and NA eliminates antigenic competition between HA and NA, as well as between N1-N2 NA ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0581

    authors： Johansson BE,Kilbourne ED

    更新日期：1996-11-01 00:00:00

  • Recent influenza A (H1N1) infections of pigs and turkeys in northern Europe.

    abstract：:The most recent introduction of an avian influenza A virus without reassortment into mammals occurred in 1979 when H1N1 strains could be isolated from diseased pigs in northern Europe. This newly introduced avian virus formed a stable lineage in pigs and, in the meantime, spread all over Europe. In 1991 highly pathoge...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1344

    authors： Ludwig S,Haustein A,Kaleta EF,Scholtissek C

    更新日期：1994-07-01 00:00:00

  • Domain- and nucleotide-specific Rev response element regulation of feline immunodeficiency virus production.

    abstract：:Computational analysis of feline immunodeficiency virus (FIV) RNA sequences indicated that common FIV strains contain a rev response element (RRE) defined by a long unbranched hairpin with 6 stem-loop sub-domains, termed stem-loop A (SLA). To examine the role of the RNA secondary structure of the RRE, mutational analy...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.04.009

    authors： Na H,Huisman W,Ellestad KK,Phillips TR,Power C

    更新日期：2010-09-01 00:00:00

  • Molecular dissection of Flock house virus protein B2 reveals that electrostatic interactions between N-terminal domains of B2 monomers are critical for dimerization.

    abstract：:Flock house virus (FHV) encodes a suppressor protein B2 to overcome antiviral RNA silencing during infection. Biochemical analyses have shown that a homodimer of B2 binds to double-stranded RNA to inhibit dicer-mediated cleavage of dsRNA and incorporation of small interfering RNAs into the RNA-induced silencing comple...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.05.023

    authors： Seo JK,Kwon SJ,Rao AL

    更新日期：2012-10-25 00:00:00

  • Interleukin 2-inducible T cell kinase (ITK) facilitates efficient egress of HIV-1 by coordinating Gag distribution and actin organization.

    abstract：:Interleukin 2-inducible T cell kinase (ITK) influences T cell signaling by coordinating actin polymerization and polarization as well as recruitment of kinases and adapter proteins. ITK regulates multiple steps of HIV-1 replication, including virion assembly and release. Fluorescent microscopy was used to examine the ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.11.015

    authors： Schiralli Lester GM,Akiyama H,Evans E,Singh J,Gummuluru S,Henderson AJ

    更新日期：2013-02-05 00:00:00

  • Reduced antigenicity of the hepatitis B virus HBsAg protein arising as a consequence of sequence changes in the overlapping polymerase gene that are selected by lamivudine therapy.

    abstract：:The prevalence of hepatitis B virus vaccine escape mutants has increased as a consequence of the introduction of global vaccination programs. Furthermore and as a consequence of the organization of the genome of hepatitis B virus (HBV) into overlapping reading frames, the selection of polymerase mutants during long-te...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1246

    authors： Torresi J,Earnest-Silveira L,Deliyannis G,Edgtton K,Zhuang H,Locarnini SA,Fyfe J,Sozzi T,Jackson DC

    更新日期：2002-02-15 00:00:00

  • Double-stranded ribonucleic acid from carnation cryptic virus.

    abstract：:The nucleic acid of carnation cryptic virus has been identified as double-stranded RNA on the basis of (i) susceptibility to RNase in 0.1 x SSC (SSC = 0.15 M NaCl-0.015 M sodium citrate buffer, pH 7.0) and resistance to RNase in 2 x SSC and to DNase, (ii) reaction with an antiserum containing antibodies to dsRNA, and ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(81)90124-0

    authors： Lisa V,Boccardo G,Milne RG

    更新日期：1981-12-01 00:00:00

  • Reovirus exists in the form of 13 particle species that differ in their content of protein sigma 1.

    abstract：:When electrophoresed in 0.7% agarose gels, populations of reovirus particles can be resolved into 13 well-defined bands that possess from 0 to 12 projection/spike-associated trimers of protein sigma 1. This state of affairs is not an artifact of purification, of the techniques used to demonstrate it, of aggregation, o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1295

    authors： Larson SM,Antczak JB,Joklik WK

    更新日期：1994-06-01 00:00:00