Abstract:
:We compared the outcome of different cellular and viral factors on the regulation of the HPV-16 early viral gene expression in trophoblastic and cervical cancer cells. A high variability of the long control (LCR) activity was observed, prompting us to evaluate the role of secreted factors in the control of the early gene expression in trophoblastic cell lines. Endogenous progesterone and exogenous dexamethasone were found to activate LCR driven transcriptional activity. Since host cells express HPV early proteins to regulate LCR activity, we investigated the effect of the combined HPV-16 early proteins on the LCR driven transcription and the possible involvement of E2. A physiological level of HPV-16 early proteins expression strongly induced the LCR driven reporter activity. According to mutational analysis, E1 and E2 proteins, indispensable for viral replication, were not involved in LCR extrachromosomal transcriptional regulation. This suggests that E5 and/or E6 and/or E7, consequently, activated viral transcription.
journal_name
Virologyjournal_title
Virologyauthors
Weyn C,Vanderwinden JM,Rasschaert J,Englert Y,Fontaine Vdoi
10.1016/j.virol.2010.12.056subject
Has Abstractpub_date
2011-03-30 00:00:00pages
146-55issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(10)00824-Xjournal_volume
412pub_type
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