Abstract:
:Red clover necrotic mosaic virus (RCNMV) encodes N-terminally overlapping proteins of 27 and 88 kDa (p27 and p88) known to be required for replication. Green fluorescent protein (GFP) fusions were used to visualize the location of p27 and p88 within Nicotiana benthamiana cells. GFP:p27 fusions localized to the endoplasmic reticulum (ER), co-localized with ER-targeted yellow fluorescent protein and caused membrane restructuring and proliferation. Cellular fractionation of virus-inoculated N. benthamiana leaves confirmed the association of p27 with ER membranes. GFP:p88 fusions also localized to the ER and co-localized with GFP:p27. Both fusion proteins co-localize to the cortical and cytoplasmic ER and were associated with invaginations of the nuclear envelope. Independent accumulation in, and perturbation of, the ER suggests that p27 and p88 function together in the replication complex. This is the first report of a member of the Tombusviridae replicating in association with the ER.
journal_name
Virologyjournal_title
Virologyauthors
Turner KA,Sit TL,Callaway AS,Allen NS,Lommel SAdoi
10.1016/j.virol.2003.12.006subject
Has Abstractpub_date
2004-03-15 00:00:00pages
276-90issue
2eissn
0042-6822issn
1096-0341pii
S0042682203009176journal_volume
320pub_type
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