Abstract:
:The nucleotide sequences of the genomes of two strains of canine adenovirus, type 1 (Glaxo and CLL) have been determined within the region spanning approximately 0.80-0.91 map unit. Homology comparisons with other adenoviral DNAs indicate that this region contains part of the hexon-associated protein (pVIII) precursor gene on the left, and a 57-kDa fiber-encoding gene on the right. In both viral strains, the sequences of these genes are identical. Accepting the generally highly conserved nature of the genetic organization of adenoviral genomes, the 1136-bp sequence between them would include the canine equivalent of the human adenoviral E3 region. In the Glaxo strain, which is nonattenuated, this sequence contains only two R-strand open reading frames capable of coding for polypeptides of 60 residues or more. One of these appears to code for a transmembrane glycoprotein of 194 amino acid residues. Neither ORF, however, shows significant homology with any other known adenoviral DNA or protein sequence. The corresponding region in the attenuated strain CLL is almost identical with that of the Glaxo, but with a 325-bp deletion resulting in a fusion of the two ORFs. It seems, therefore, that neither ORF is essential for viral replication in vitro, since the CLL strain replicates just as efficiently as the Glaxo. The suggestion is made that the attenuation of the CLL strain may be related to the nonfunctionality of its E3 gene product(s).
journal_name
Virologyjournal_title
Virologyauthors
Dragulev BP,Sira S,Abouhaidar MG,Campbell JBdoi
10.1016/0042-6822(91)90142-xsubject
Has Abstractpub_date
1991-07-01 00:00:00pages
298-305issue
1eissn
0042-6822issn
1096-0341journal_volume
183pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Studies were performed to induce cross-clade neutralizing antibodies (Abs) by testing various combinations of prime and boost constructs that focus the immune response on structurally-conserved epitopes in the V3 loop of HIV-1 gp120. Rabbits were immunized with gp120 DNA containing a V3 loop characterized by the GPGR ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.039
更新日期:2009-09-15 00:00:00
abstract::Genetic exchanges constitute a significant means by which bacteriophages acquire novel characteristics. Phages of Lactococcus lactis occupy a particular niche, the dairy factory environment, where their populations are subjected to constant changes. Little is known about the mechanisms of evolution that lead to the ge...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0226
更新日期:2000-04-25 00:00:00
abstract::Neutralizing monoclonal antibodies 2F1 and 1C10, which are specific for VP7 of serotype 2 rotaviruses (G2), were used to select neutralization escape variants of the human serotype 2 rotavirus, DS-1. Neutralization survival patterns generated by monoclonal antibodies 2F1, 1C10, and RV5:3 indicated that 2F1 and 1C10 di...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1601
更新日期:1993-11-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) exploits multiple host factors during its replication. The REV/RRE-dependent nuclear export of unspliced/partially spliced viral transcripts needs the assistance of host proteins. Recent studies have shown that MOV10 overexpression inhibited HIV-1 replication at various step...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.08.026
更新日期:2015-12-01 00:00:00
abstract::Gene transfer approaches using viruses such human adenovirus (HAdV) may provide an alternative treatment for diseases involving hematopoietic cells. Better understanding of the cellular mechanisms by which the HAdV introduces DNA into these cells should help in vector design. We examined HAdV intracellular delivery in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.09.043
更新日期:2004-03-01 00:00:00
abstract::Unlike cell lines transformed with Polyomaviruses, transformants derived by focus formation or colony formation in agarose medium after transfer into rat fibroblast cells (FR3T3 line) of Bovine Papilloma Type 1 (BPV1) DNA were consistently observed to grow poorly in suspension and to remain highly serum dependent for ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90196-x
更新日期:1984-06-01 00:00:00
abstract::The cellular protein Cyclophilin A (Cyp A) is packaged into human immunodeficiency virus type 1 (HIV-1) particles through a specific interaction with the capsid domain of the Gag polyprotein. Inhibition of Cyp A incorporation by mutagenesis or cyclosporin treatment severely affects infectivity of all HIV-1 M subtypes ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1347
更新日期:2002-03-15 00:00:00
abstract::MuSV349 is a TB-cell line which produces infectious MuSV with little or no MuLV detectable by the XC assay. The apparent restriction of MuLV replication in MuSV349 cells was investigated. A replication-competent helper virus, with protein composition nearly identical to that of Mo-MuLV was isolated from the viruses pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90043-6
更新日期:1984-01-30 00:00:00
abstract::In retrovirus-induced tumors, proviral DNA is commonly found next to or within a cellular proto-oncogene such that transcription of the gene is influenced by the viral promoter or enhancer. Extensive surveys of naturally occurring tumors reveal that proviruses integrated on the 3' side of the gene are usually in the s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1245
更新日期:1993-05-01 00:00:00
abstract::Cytotoxic T-lymphocyte (CTL) epitopes within the HIV genome are subject to negative and positive selective pressures, the balance of which influences CTL escape at a given epitope. We investigated whether viral fitness requirements dictate conservation of the HLA-A2 restricted immunodominant epitope SLYNTVATL (SL9). V...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.03.003
更新日期:2009-05-25 00:00:00
abstract::Using reverse genetics methods, we constructed three different transfectant influenza A viruses encoding an Ld-restricted, nine amino-acid-long fragment, corresponding to amino-acid residues 876-884, of beta-galactosidase (beta-gal). Sequences encoding this epitope were nested within the hemagglutinin (HA) or neuramin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9330
更新日期:1998-09-15 00:00:00
abstract::The main route of small ruminant lentivirus dissemination is the ingestion of infected cells present in colostrum and milk from infected animals. However, whether only macrophages or other cell subtypes are involved in this transmission is unknown. We derived epithelial cell cultures, 100% cytokeratin positive, from m...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9752
更新日期:1999-06-20 00:00:00
abstract::Two bipartite begomoviruses, Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV), have been isolated from mosaic-diseased cassava originating from central India and Sri Lanka, respectively. ICMV was transmitted with low efficiency from cassava to Nicotiana benthamiana by sap inoculation to g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1251
更新日期:2002-02-01 00:00:00
abstract::Interleukin (IL)-15, is a cytokine that is important for the maintenance of long-lasting, high-avidity T cell response to invading pathogens and has, therefore, been used in vaccine and therapeutic platforms as an adjuvant. In addition to pure protein delivery, plasmids encoding the IL-15 gene have been utilized. Howe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.017
更新日期:2009-10-10 00:00:00
abstract::The polycation DEAE-dextran increased the binding of VSV to BHK cells about fourfold over a wide range of VSV concentrations. The same proportion of bound virions was internalized by the cell in the presence or absence of DEAE-dextran. Viral primary RNA production was increased to the equivalent of a 4- to 4.5-fold in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90429-x
更新日期:1984-02-01 00:00:00
abstract::Human parvovirus B19 (B19) infects human erythroid lineage cells. Accumulating evidence also shows that B19 is detectable in nonerythroid lineage cells in vivo, but the mechanism of infection is still not clear. In this study, we explored the mode of B19 infection of human monocytic cell line U937. An in vitro infecti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.09.040
更新日期:2006-02-05 00:00:00
abstract::In HIV-1 infection, plasma viral load (VL) has dual implications for pathogenesis and public health. Based on well-known patterns of HIV-1 evolution and immune escape, we hypothesized that VL is an evolving quantitative trait that depends heavily on duration of infection (DOI), demographic features, human leukocyte an...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.11.024
更新日期:2014-01-20 00:00:00
abstract::The mechanism by which lentivirus envelope (Env) glycoproteins are packaged into budding virions is poorly understood. Simian immunodeficiency virus (SIV) contains an Env protein with an unusually long cytoplasmic tail. To investigate the role of this domain in the incorporation of the SIV Env into virions, we generat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0869
更新日期:2001-05-10 00:00:00
abstract::Bovine respiratory disease (BRD) is the costliest disease affecting the cattle industry globally. Orthomyxoviruses, influenza C virus (ICV) and influenza D virus (IDV) have recently been implicated to play a role in BRD. However, there are contradicting reports about the association of IDV and ICV to BRD. Using the la...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.08.014
更新日期:2020-12-01 00:00:00
abstract::All lepidopteran baculovirus genomes sequenced to date encode a viral fibroblast growth factor homolog (vfgf), suggesting that vfgf may play an important role in the infection cycle of lepidopteran baculoviruses. Here, we describe the characterization of a Bombyx mori nucleopolyhedrovirus (BmNPV) mutant lacking functi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.07.008
更新日期:2006-11-10 00:00:00
abstract::The mature flavivirus capsid protein (virion C) is commonly thought to be free in the cytoplasm of infected cells and to form a nucleocapsid-like complex with genomic RNA in mature virus particles. There is little sequence conservation among flavivirus virion C proteins, but they are similar in size (e.g., 99 amino ac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8608
更新日期:1997-06-23 00:00:00
abstract::Two small viral proteins (DGBp1 and DGBp2) have been proposed to act in a concerted manner to aid intra- and intercellular trafficking of carmoviruses though the distribution of functions and mode of action of each protein partner are not yet clear. Here we have confirmed the requirement of the DGBps of Pelargonium fl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.03.001
更新日期:2011-05-10 00:00:00
abstract::Transcription of the paramyxovirus RNA genome is thought to involve a sequential stop-start mechanism whereby monocistronic mRNAs are produced by polyadenylation and termination of 3' upstream gene followed by reinitiation at the downstream start site. For a number of paramyxoviruses, transcription across the M-F gene...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9266
更新日期:1998-08-01 00:00:00
abstract::Two minor proteins with molecular masses of 8.5 and 15.5 kDa were identified in feline calicivirus (FCV) virions. Direct sequence analysis showed that the N-terminal sequence of the 8.5-kDa protein was identical to that of the predicted protein encoded by open reading frame 3 (ORF3) of the FCV genome. The N-terminal s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0579
更新日期:2000-11-10 00:00:00
abstract::Cauliflower mosaic virus (CaMV) strains CM1841 and W260 produced markedly different symptoms when inoculated onto turnips (Brassica campestris L. 'Just Right'). The CM1841 strain induced a mild degree of stunting of infected plants while strain W260 caused moderate to severe stunting. Although CM1841 was significantly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90898-l
更新日期:1991-04-01 00:00:00
abstract::The nucleotide sequence of two variants of coconut tinangaja viroid (CTiV) were obtained. Both sequence variants are 254 nucleotide residues in size but differ in sequence at two positions. In comparisons with other viroids, the sequences and proposed secondary structure of CTiV show most homology with the 246 nucleot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90497-7
更新日期:1988-02-01 00:00:00
abstract::It has been shown that the genomes of episomally maintained DNA viruses are tethered to host cell chromosomes during cell division, facilitating maintenance in dividing cells. The papillomavirus E2 protein serves this mechanism of viral genome persistence by simultaneously associating with chromatin and the viral geno...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.03.015
更新日期:2011-05-25 00:00:00
abstract::The single-stranded DNA-binding protein LEF-3 of Autographa californica multinucleocapsid nucleopolyhedrovirus consists of 385 amino acid residues, forms oligomers, and promotes Mg2+-independent unwinding of DNA duplexes and annealing of complementary DNA strands. Partial proteolysis revealed that the DNA-binding doma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.027
更新日期:2006-03-15 00:00:00
abstract::Lymphocytic choriomeningitis virus (LCMV) Armstrong caused disordered growth and glucose metabolism secondary to growth hormone deficiency in infected C3H/St mice. In contrast, LCMV strain WE replicated in fewer growth hormone-producing cells, failed to disrupt growth hormone synthesis, and did not cause growth hormon...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90431-3
更新日期:1985-04-15 00:00:00
abstract::RNA-binding proteins of coronavirus MHV-A59 were identified using an RNA overlay-protein blot assay (ROPBA). The major viral RNA-binding protein in virions and infected cells was the phosphorylated nucleocapsid protein N (50K). A new 140K virus structural protein was identified as a minor RNA-binding protein both in v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90305-3
更新日期:1986-04-30 00:00:00