Abstract:
:The main route of small ruminant lentivirus dissemination is the ingestion of infected cells present in colostrum and milk from infected animals. However, whether only macrophages or other cell subtypes are involved in this transmission is unknown. We derived epithelial cell cultures, 100% cytokeratin positive, from milk of naturally infected and noninfected goats. One such culture, derived from a naturally infected goat, constitutively produced a high titer of virus in the absence of any cytopathic effect. The other cultures, negative for natural lentivirus infection, were tested for their susceptibility to infection with the CAEV-CO strain and a French field isolate CAEV-3112. We showed that milk epithelial cells are easily infected by either virus and produce viruses at titers as high as those obtained in permissive goat synovial membrane cells. The CAEV-CO strain replicated in milk epithelial cells in absence of any cytopathic effect, whereas the CAEV-3112 field isolate induced both cell fusion and cell lysis. Our results suggest that CAEV-infected milk epithelial cells of small ruminants may play an important role in virus transmission and pathogenesis.
journal_name
Virologyjournal_title
Virologyauthors
Mselli-Lakhal L,Guiguen F,Fornazero C,Du J,Favier C,Durand J,Grezel D,Balleydier S,Mornex JF,Chebloune Ydoi
10.1006/viro.1999.9752subject
Has Abstractpub_date
1999-06-20 00:00:00pages
67-73issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(99)99752-0journal_volume
259pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Monoclonal antibodies directed against glycoprotein D of herpes simplex virus completely inhibited fusion of Vero cells infected with type 1 virus. In contrast, several monoclonal antibodies directed against other viral glycoproteins, including B, were ineffective or were only minimally inhibitory at the highest conce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90409-9
更新日期:1983-08-01 00:00:00
abstract::The bovine papilloma virus (BPV) E1 protein essential to viral DNA replication has recently been shown to associate via direct protein-DNA interactions with the viral origin of replication and to be an ATP-dependent helicase. We show here that in accordance with the latter function, the E1 gene product has intrinsic A...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1544
更新日期:1994-10-01 00:00:00
abstract::To investigate the mode of gene expression of red clover mottle virus (RCMV) middle component (M) RNA, we have synthesized an oligopeptide corresponding to the predicted carboxy-terminus of the RCMV counterparts of the cowpea mosaic virus (CPMV) 48K and 58K proteins. Using an antiserum raised against this synthetic ol...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90552-7
更新日期:1989-12-01 00:00:00
abstract::Hepatitis C virus (HCV) encodes a polyprotein that is processed to produce the structural and nonstructural proteins of the virus. Nonstructural protein 3 (NS3) is a serine proteinase that cleaves the polyprotein to release the NS4A, NS4B, NS5A, and NS5B proteins. To characterize the substrate specificity of NS3, we s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1520
更新日期:1994-10-01 00:00:00
abstract::The results of a previous study suggested that the poly(A) sequence in barley stripe mosaic virus (BSMV) RNA is intercalated between a 3'-terminal tyrosine-accepting structure and the 5'-terminal coding part of the BSMV genome. Here we show that poly(A)+ and poly(A)- fractions of BSMV RNA can be cleaved into two fragm...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90064-2
更新日期:1982-05-01 00:00:00
abstract::At least four hantavirus species cause disease with prominent renal involvement-hemorrhagic fever with renal syndrome (HFRS); and several hantavirus strains cause disease with significant pulmonary involvement-hantavirus pulmonary syndrome (HPS). The most prevalent and lethal hantaviruses associated with HFRS and HPS ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.035
更新日期:2006-03-30 00:00:00
abstract::Activation of the human cytomegalovirus (HCMV) origin of replication (oriLyt) was previously demonstrated in transient transfection assays in permissive human fetal fibroblasts and nonpermissive Vero cells, and shown to require six viral proteins that function at the replication fork plus a number of HCMV products tha...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00005-9
更新日期:2003-04-10 00:00:00
abstract::The nucleotide sequences of the long terminal repeat (LTR) of simian retroviruses (STLV), which are closely related to human T-cell leukemia virus type I (HTLV-I) were found to be of at least two subgroups: an Asian subtype in macaques and an African subtype in African green monkeys and chimpanzee. The nucleotide sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90336-3
更新日期:1986-01-30 00:00:00
abstract::Genotype VI-paramyxovirus (GVI-PMV1) is a major cause of epidemic Newcastle-like disease in Columbiformes. This genotype of avian paramyxovirus type 1 has diversified rapidly since its introduction into the US in 1982 resulting in two extant lineages, which have different population growth properties. Although some GV...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.06.007
更新日期:2014-08-01 00:00:00
abstract::The International Human Papillomavirus (HPV) Reference Center supports quality and order in HPV research and diagnostics. Notably, the center assigns HPV type numbers to novel HPV types, maintains a reference clone repository, and issues international proficiency panels for HPV genotyping. The established HPV types, c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.04.003
更新日期:2018-06-01 00:00:00
abstract::A short upstream open reading frame (uORF2) in the human cytomegalovirus (CMV) gpUL4 (gp48) transcript leader is conserved among CMV strains and inhibits translation of a downstream cistron. Remarkably, this inhibitory effect depends on the amino acid coding information of uORF2, at least in transient transfection ass...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1630
更新日期:1994-11-15 00:00:00
abstract::We have examined FIV vif function in the context of molecular clone pF34 (GenBank Accession No. M25729). Rabbit antisera directed against the translation product of the vif gene identified a 29-kDa protein in tissue culture cells infected with FIV-pF34; this protein was not present in cultures of uninfected cells. Thu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1609
更新日期:1994-11-01 00:00:00
abstract::Viral vector particles derived from murine leukemia virus (MLV) mediate highly efficient stable gene transfer used in gene therapeutic approaches and in the generation of transgenic cell lines. However, the establishment of stable viral packaging cells (VPCs) is a time-consuming challenge. To overcome this limitation,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.02.014
更新日期:2019-05-01 00:00:00
abstract::Methods currently used for HIV-1 viral load measurements are very sensitive, but cannot distinguish between infectious and noninfectious particles. Here we describe the development of a novel, sensitive, and highly reproducible method that allows rapid isolation and quantification of infectious particles from patient ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.05.022
更新日期:2004-08-15 00:00:00
abstract::Two small viral proteins (DGBp1 and DGBp2) have been proposed to act in a concerted manner to aid intra- and intercellular trafficking of carmoviruses though the distribution of functions and mode of action of each protein partner are not yet clear. Here we have confirmed the requirement of the DGBps of Pelargonium fl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.03.001
更新日期:2011-05-10 00:00:00
abstract::Expression of Kaposi's sarcoma herpesvirus vFLIP, a potent activator of NFkB signaling, promotes latency. Inhibition of NFkB signaling promotes lytic reactivation. We previously reported that lytic inducer, RTA, inhibits vFLIP induced NFkB signaling by inducing the degradation of vFLIP via the proteasome. Here we repo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.11.016
更新日期:2017-01-15 00:00:00
abstract::Retroviruses can be grouped by viral interference measurements into classes which use common cell surface receptors. We previously tested a large number of isolates of mink cell focus-inducing (MCF) murine leukemia viruses (MuLVs), and reported that all of them share a distinct receptor on NIH/3T3 cells (A. Rein, Viro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90255-1
更新日期:1984-07-15 00:00:00
abstract::About 100 nucleotides of DNA sequence at the 5' noncoding region of the Choristoneura biennis entomopoxvirus spheroidin gene was chemically synthesized and inserted into a vaccinia expression vector, interrupting the vaccinia thymidine kinase gene. When the bacterial beta-galactosidase gene was introduced downstream o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90070-r
更新日期:1991-02-01 00:00:00
abstract::Transient expression studies using a Nicotiana benthamiana suspension cell-derived protoplast system have identified a minimal sequence that is necessary and sufficient for activation of the tomato golden mosaic virus coat protein (CP) promoter by the viral TrAP protein (also called AL2). The sequence has a bipartite ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1757
更新日期:2003-01-20 00:00:00
abstract::Human papillomavirus type 27 (HPV-27) and HPV-2c are isolates of the same virus as judged by genomic sequence identity. In a 400-bp stretch of the long control region and the E6 and L1 genes no changes were found between HPV-27 and HPV-2c whereas 55 changes were found between HPV-27 and HPV-2a. This justifies the elev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1307
更新日期:1994-06-01 00:00:00
abstract::Demyelination of the human peripheral nervous system (PNS) can be caused by diverse mechanisms including viral infection. Despite association of several viruses with the development of peripheral demyelination, animal models of the condition have been limited to disease that is either autoimmune or genetic in origin. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.09.009
更新日期:2007-03-01 00:00:00
abstract::New BVDV strains associated with very high mortalities, which killed about 25% of the veal calves in Quebec in 1993, have been isolated. In this study, characterization of the last two-thirds of the 5' untranslated region (5'UTR) of their genome and virus neutralization experiments with polyvalent antisera raised in d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1483
更新日期:1994-09-01 00:00:00
abstract::The immortalizing function of the human adenovirus 5 E1A oncoprotein requires efficient localization to the nucleus. In 1987, a consensus monopartite nuclear localization sequence (NLS) was identified at the C-terminus of E1A. Since that time, various experiments have suggested that other regions of E1A influence nucl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.08.014
更新日期:2014-11-01 00:00:00
abstract::Murine monoclonal antibodies were developed against the protein products produced by murine C127 cells which had been transfected with a recombinant plasmid clone containing the human T-cell leukemia (lymphotropic) virus type I (HTLV-I) proviral DNA coding regions for part of env, px, and the 3' LTR. Four antibodies w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90270-9
更新日期:1986-04-15 00:00:00
abstract::Disruption of the perforin gene results in primary immunodeficiency and an increased susceptibility to opportunistic pathogens. Perforin-deficient (PKO) mice fail to clear primary lymphocytic choriomeningitis virus (LCMV) Armstrong, resulting in persistent infection and functional exhaustion of virus-specific CD8+ T c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.038
更新日期:2008-01-20 00:00:00
abstract::The OC43 strain of human coronavirus (HCoV-OC43) is an ubiquitous respiratory tract pathogen possessing neurotropic capacities. Coronavirus structural envelope (E) protein possesses specific motifs involved in protein-protein interaction or in homo-oligomeric ion channel formation, which are known to play various role...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.12.023
更新日期:2018-02-01 00:00:00
abstract::The formation of a lipoprotein membrane within specialized areas of the cytoplasm is the first visible step in poxvirus morphogenesis. The A17L viral protein, an essential nonglycosylated membrane component, was predicted to have four centrally located alpha-helical membrane-spanning domains. The gene was expressed as...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9870
更新日期:1999-09-01 00:00:00
abstract::Measles virus transcription generates multiple P/C gene-specific mRNAs by a process which has been termed editing. In one of these mRNAs, the cotranscriptional addition of a single nontemplated G residue allows translational access to the V protein reading frame. The protein translated from this mRNA has been called V...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1050
更新日期:1994-01-01 00:00:00
abstract::Proviruses were cloned directly from a cat that developed neurological disorders approximately 28 months after inoculation with a molecularly cloned, minimally pathogenic subgroup A feline leukemia virus (FeLV-A). In addition to FeLV-A proviruses that were nearly identical to the inoculated virus, we detected a subgro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1597
更新日期:1994-11-01 00:00:00
abstract::Transmissible gastroenteritis virus (TGEV) causes severe diarrhea and high mortality in newborn piglets. It is well established that porcine intestinal epithelium is the target of the TGEV infection, however the mechanism that TGEV invades the host epithelium remains largely unknown. Aminopeptidase N (APN) is a known ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.05.009
更新日期:2018-08-01 00:00:00