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Functional analysis of the C-terminal region of human adenovirus E1A reveals a misidentified nuclear localization signal.

Abstract：

:The immortalizing function of the human adenovirus 5 E1A oncoprotein requires efficient localization to the nucleus. In 1987, a consensus monopartite nuclear localization sequence (NLS) was identified at the C-terminus of E1A. Since that time, various experiments have suggested that other regions of E1A influence nuclear import. In addition, a novel bipartite NLS was recently predicted at the C-terminal region of E1A in silico. In this study, we used immunofluorescence microscopy and co-immunoprecipitation analysis with importin-α to verify that full nuclear localization of E1A requires the well characterized NLS spanning residues 285-289, as well as a second basic patch situated between residues 258 and 263 ((258)RVGGRRQAVECIEDLLNEPGQPLDLSCKRPRP(289)). Thus, the originally described NLS located at the C-terminus of E1A is actually a bipartite signal, which had been misidentified in the existing literature as a monopartite signal, altering our understanding of one of the oldest documented NLSs.

journal_name

Virology

journal_title

Virology

authors

Cohen MJ,King CR,Dikeakos JD,Mymryk JS

doi

10.1016/j.virol.2014.08.014

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

238-243

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(14)00385-7

journal_volume

468-470

pub_type

杂志文章

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