Abstract:
:Mapping of the 5' ends of virion-derived hepatitis B virus DNA molecules was carried out after cloning and sequencing linearized genomes made fully double stranded. Of five clones obtained, three of four minus strand termini mapped to the G in position 1826 and one to the T in position 1827. Plus strand 5' ends were more heterogeneous with evidence from one clone for the presence of an RNA-translocated primer from the 5' end of pregenomic RNA as well as two other termini, mapped to positions 1599 and 1601, the second 3' nucleotide of DR2 and the first nucleotide 3' to DR2, respectively. These molecules were also shown to be functional templates for expression of HBcAg after tranfection or microinjection of Huh-7 human hepatoma cells.
journal_name
Virologyjournal_title
Virologyauthors
Saldanha JA,Qiu H,Thomas HC,Monjardino Jdoi
10.1016/0042-6822(92)90765-hsubject
Has Abstractpub_date
1992-05-01 00:00:00pages
358-61issue
1eissn
0042-6822issn
1096-0341journal_volume
188pub_type
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