Abstract:
:Norwalk virus (NV) is the prototype strain of a group of noncultivatable caliciviruses that infect humans and cause outbreaks of epidemic acute nonbacterial gastroenteritis. The NV virion is composed of 180 copies of a single structural protein that, when expressed in insect cells infected with a recombinant baculovirus, assembles into empty recombinant Norwalk virus-like particles (rNV VLPs) which are morphologically and antigenically similar to native NV. We have begun to dissect the antigenic structure of the rNV particles using monoclonal antibodies made to the rNV VLPs. Ten MAbs made to rNV particles were characterized for their reactivity as detector antibodies by ELISA, as capture antibodies in an ELISA to detect NV in stools, by Western blot, and by immunoprecipitation. Seven of the MAbs recognize discontinuous epitopes, requiring the rNV capsid protein to remain at least partially folded, while the other three recognize continuous epitopes. Eight of the MAbs map to the C-terminal half of the capsid protein as they react by Western blot and by immunoprecipitation with a 32K trypsin cleavage product of the full-length 58K capsid protein, suggesting that the C-terminal half of the capsid protein may contain the immunodominant epitopes. The three MAbs that recognize continuous epitopes map to the extreme C terminus of the capsid protein, between amino acids 457 and 530, in a region that is relatively conserved among different human calicivirus capsid proteins. These MAbs which were assigned into three antigenic groups will be useful as tools to further dissect the structural and antigenic topography of the NV virion, and as unlimited reagents to detect NV in diagnostic assays.
journal_name
Virologyjournal_title
Virologyauthors
Hardy ME,Tanaka TN,Kitamoto N,White LJ,Ball JM,Jiang X,Estes MKdoi
10.1006/viro.1996.0112subject
Has Abstractpub_date
1996-03-01 00:00:00pages
252-61issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(96)90112-9journal_volume
217pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Phage-display methodology has been used to select variant CD4 proteins exhibiting increased binding to the surface envelope glycoprotein, gp120, of Human Immunodeficiency Virus Type-1. To facilitate the selection, a library of mutant CD4 proteins was constructed by cloning a PCR-generated error prone population of the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8651
更新日期:1997-08-04 00:00:00
abstract::Murine monoclonal antibodies were developed against the protein products produced by murine C127 cells which had been transfected with a recombinant plasmid clone containing the human T-cell leukemia (lymphotropic) virus type I (HTLV-I) proviral DNA coding regions for part of env, px, and the 3' LTR. Four antibodies w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90270-9
更新日期:1986-04-15 00:00:00
abstract::Progressive reorganization and depolymerization of microtubules corresponded with virus-induced rounding of Autographa californica M nuclear polyhedrosis virus (AcMNPV)-infected Spodoptera frugiperda IPLB-Sf-21 cells, suggesting that microtubules were instrumental in maintaining the normal shape of these cells. Depoly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90211-9
更新日期:1990-03-01 00:00:00
abstract::To better understand the origin of human T-cell leukemia virus type l (HTLV-l) in South America, we conducted a phylogenetic study on 27 new HTLV-ls in Brazil. These were obtained from Brazilians of various ethnic origins, such as Japanese immigrants, whites, blacks and mulattos. We amplified and sequenced proviral DN...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1999-08-15 00:00:00
abstract::Here, we evaluated the role of two host proteins, a Bromo domain containing RNA binding protein (BRP1) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), in the replication of Cucumber mosaic virus (CMV). LC-MS/MS analysis of host/viral proteins pull down against BRP1 from CMV-infected plants co-infiltrated with BR...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.04.001
更新日期:2016-07-01 00:00:00
abstract::Segment 7 (M) of the cold-adapted live influenza A virus vaccine plays a primary role in the ability of this virus to interfere with the replication of wild-type influenza A viruses. This conclusion is based on several lines of evidence. Single gene reassortant viruses derived by crossing influenza A/Ann Arbor/6/60 (H...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90011-y
更新日期:1991-01-01 00:00:00
abstract::Visna is a slow virus infection of sheep in which the characteristic pathological change is demyelination in foci of inflammation. The latter is thought to be the result of an immunopathological process directed against cellular and antigenic targets that have been difficult to define because of restricted viral gene ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90264-8
更新日期:1985-03-01 00:00:00
abstract::The lentivirus caprine arthritis-encephalitis virus (CAEV) is closely related by nucleotide sequence homology to visna virus and other sheep lentiviruses and shows less similarity to the other animal and human lentiviruses. The genomic organization of CAEV is very similar to that of visna virus and the South African o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90303-9
更新日期:1990-11-01 00:00:00
abstract::Previous work (C.F. Spiropoulou and S.T. Nichol, 1993, J. Virol. 67, 3103-3110) has demonstrated the existence in cells infected with the New Jersey serotype of vesicular stomatitis virus (VSV) of two small carboxy-coterminal proteins encoded by the P mRNA. These proteins have been named C' and C. We are interested in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0202
更新日期:1996-04-15 00:00:00
abstract::The adenovirus (Ad) single-stranded DNA binding protein (DBP) is a multifunctional protein. It is thought to consist of two domains, the amino-terminal domain involved in host-range determination and the carboxyl-terminal domain functioning in DNA replication and DNA binding. We have determined the nucleotide sequence...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90227-9
更新日期:1988-03-01 00:00:00
abstract::Hepatitis delta virus (HDV) encodes two proteins, the small hepatitis delta antigen (SHDAg) and large hepatitis delta antigen (LHDAg). Both proteins are identical except for the presence of additional 19 amino acids at the C terminus of LHDAg. While SHDAg is required for HDV RNA replication, LHDAg inhibits replication...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1109
更新日期:1994-02-15 00:00:00
abstract::The tegument protein pp28 of human cytomegalovirus (HCMV) is essential for the assembly of infectious HCMV virions, but how it functions during the process of HCMV tegumentation and envelopment remains unclear. By using live cell fluorescence resonance energy transfer (FRET) microscopy and yeast two-hybrid assays, we ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.03.007
更新日期:2009-05-25 00:00:00
abstract::During replication, the lengthy genome of dsDNA viruses is translocated with remarkable velocity into the limited space within the preformed procapsid. We previously found that a viral-encoded RNA (pRNA) played a key role in bacterial virus phi29 DNA translocation. Design of mutant pRNA sets containing two and three i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0771
更新日期:2001-03-15 00:00:00
abstract::Respiratory syncytial virus (RSV) induces the production of a number of cytokines and chemokines by activation of nuclear factor kappa B (NF-kappaB). The activation of NF-kappaB has been shown to depend on viral replication in the infected cells. In this study, we demonstrate that expression of RSV M2-1 protein, a tra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.10.031
更新日期:2005-01-20 00:00:00
abstract::Rabbit and human papillomaviruses are strictly epitheliotropic and their DNA replicates extrachromosomally in benign lesions (warts) of their natural host. Their tissue and host specificity is thought to be genetically controlled and may account for the inefficiency in transforming heterologous cells. In our hands, CR...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90149-8
更新日期:1987-12-01 00:00:00
abstract::Encephalomyocarditis virus (EMCV) is the prototype member of the cardiovirus genus of picornaviruses. For cardioviruses and the related aphthoviruses, the first protein segment translated from the plus-strand RNA genome is the Leader protein. The aphthovirus Leader (173-201 amino acids) is an autocatalytic papain-like...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1193
更新日期:2001-11-25 00:00:00
abstract::In the absence of an effective vaccine against HIV, it is urgent to develop an effective alternative such as a microbicide. Single and repeated applications of MC1220 microbicide were evaluated in macaques. First, animals were given a single application of 0.5% or 1.5% MC1220-containing liposomal gel. A second group w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.06.008
更新日期:2010-09-15 00:00:00
abstract::We examined the molecular epidemiology and evolution of Ross River (RR) virus in Australia and the Pacific Islands. Nucleotide sequences of the E2 and E3 genes of five RR virus strains revealed remarkable conservation between 1959 and 1989 with a maximum divergence of only 3.3%. Sequence data from a 505-base pair frag...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1449
更新日期:1995-09-10 00:00:00
abstract::The lung-derived dendritic cell (LDC) recruitment following intranasal (i.n.) vaccination of different poxviral vector-based vaccines/adjuvants were evaluated to decipher how these factors influenced CD8(+) T cell avidity. Compared to the standard i.n. recombinant fowlpox virus (FPV)-HIV vaccination, the FPV-HIV IL-13...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.09.004
更新日期:2014-11-01 00:00:00
abstract::Antisera with Fiji disease virus (FDV) protein-specific and double-stranded (ds) RNA-specific antibodies were prepared by injecting rabbits with highly purified preparations of FDV cores. Electron microscopic examination of negatively stained core preparations disclosed the presence of icosahedral particles, about 54 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90495-1
更新日期:1979-06-01 00:00:00
abstract::Nucleos(t)ide analogues that inhibit hepatitis B virus (HBV) DNA replication are typically used as monotherapy for chronically infected patients. Treatment with a nucleos(t)ide analogue eliminates most HBV DNA replication intermediates and produces a gradual decline in levels of covalently closed circular DNA (cccDNA)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.08.014
更新日期:2013-11-01 00:00:00
abstract::BS-1, a stroma cell line derived from normal human bone marrow, can support the growth of murine erythroid (BFU-E), granulocyte-macrophage (CFU-GM), and megakaryocyte (CFU-Meg) progenitor cells in a short-term in vitro coculture system. Exposure of BS-1 cell to the human immunodeficiency virus (HIV) prior to coculture...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1159
更新日期:1993-03-01 00:00:00
abstract::The oncogenic potential of human papillomavirus type 18 which is found in a significant number of cervical and penile cancer biopsies was tested in primary human keratinocytes derived from neonatal foreskin. Viral DNA and a gene for resistance to neomycin were introduced into these cells by calcium phosphate transfect...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90247-x
更新日期:1989-11-01 00:00:00
abstract::Essential host cofactors in retrovirus replication bind cis-acting sequences in the 5'untranslated region (UTR). Although host RBPs are crucial to all aspects of virus biology, elucidating their roles in replication remains a challenge to the field. Here RNA affinity-coupled-proteomics generated a comprehensive, unbia...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.06.001
更新日期:2015-12-01 00:00:00
abstract::HIV-1 entry into cells requires the interaction of both HIV-1 envelope proteins and membrane lipids. We investigated the mechanism of neutralization of HIV-1 infection of primary monocyte-derived macrophages (MDM) by a murine monoclonal antibody (mAb) WR321. WR321 specifically binds phosphatidylinositol-4-phosphate an...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.04.017
更新日期:2012-09-01 00:00:00
abstract::It is well established that +ssRNA viruses manipulate cellular lipid homoeostasis and distribution to facilitate efficient replication. Here, we show that the cellular lipid ceramide is redistributed to the West Nile virus strain Kunjin virus (WNVKUN) replication complex (RC) but not to the dengue virus serotype 2 str...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.06.015
更新日期:2015-10-01 00:00:00
abstract::The PstI-N genomic fragment of Autographa californica nuclear polyhedrosis virus (AcNPV) encodes an immediate-early protein, IE2, that functions as a promiscuous transactivator of other early viral promoters and heterologous promoters both in vivo and in vitro. IE2 contains several sequence motifs that are common to t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1332
更新日期:1994-07-01 00:00:00
abstract::The members of the c-myb proto-oncogene family encode sequence-specific transcriptional activators. In T cells, expression of c-myb and the related B-myb gene is induced following mitogenic stimulation. Using a purified recombinant protein, we report here that the human T cell lymphotropic virus type 1 (HTLV-1) LTR c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90044-p
更新日期:1992-02-01 00:00:00
abstract::The CD4+ T-cell-restricted recognition of the structural proteins in the JHM strain of MHV (JHMV) was compared between two mouse strains. Following immunization with inactivated JHMV, all proteins elicited an immune response in both C57BL/6 and BALB/c mouse strains, except the nucleocapsid (N) protein, which was not i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0608
更新日期:1996-11-15 00:00:00
abstract::Nectin-1 is a receptor for herpes simplex virus (HSV), a member of the immunoglobulin superfamily, and a cellular adhesion molecule. To study domains of nectin-1alpha involved in cell fusion, we measured the ability of nectin-1alpha/nectin-2alpha chimeras, nectin-1alpha/CD4 chimeras, and transmembrane domain and cytop...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.05.031
更新日期:2005-09-01 00:00:00