Abstract:
:The PstI-N genomic fragment of Autographa californica nuclear polyhedrosis virus (AcNPV) encodes an immediate-early protein, IE2, that functions as a promiscuous transactivator of other early viral promoters and heterologous promoters both in vivo and in vitro. IE2 contains several sequence motifs that are common to transcriptional activators. We have employed site-directed mutagenesis coupled with transient-expression assays to identify the amino acid sequences of IE2 that are essential for transactivation. Sequential deletion of amino-terminal sequences of IE2 gradually decreased the activity of the protein. Carboxy-terminal truncations of IE2 resulted in a dramatic loss of transactivation activities. Analysis of IE2 internal-deletion mutants demonstrated that the acidic amino acid-rich region between amino acids 198 and 206 possessed significant transactivation potential for all target promoters tested. In addition, squelching phenomena exhibited by wild-type IE2 and IE2 deletions containing the acidic amino acid-rich region indirectly demonstrated that this region may be involved in interactions with factors of the basic transcription machinery.
journal_name
Virologyjournal_title
Virologyauthors
Yoo S,Guarino LAdoi
10.1006/viro.1994.1332subject
Has Abstractpub_date
1994-07-01 00:00:00pages
164-72issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(84)71332-8journal_volume
202pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Virus infection may contribute to asthma pathogenesis. In turn, a Th2-polarized pulmonary environment may increase host susceptibility to infection. We used a cockroach antigen (CRA) model of allergic airway disease to test the hypothesis that Th2 cytokine overproduction increases susceptibility to mouse adenovirus ty...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.009
更新日期:2009-08-15 00:00:00
abstract::Transmissible gastroenteritis virus (TGEV) causes severe diarrhea and high mortality in newborn piglets. It is well established that porcine intestinal epithelium is the target of the TGEV infection, however the mechanism that TGEV invades the host epithelium remains largely unknown. Aminopeptidase N (APN) is a known ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.05.009
更新日期:2018-08-01 00:00:00
abstract::We have cloned an infectious genome of the Junonia coenia densonucleosis virus (JcDNV) into the bacterial plasmid pBR322. The viral genome could be rescued from the recombinant plasmid pBRJ by transfection of pBRJ DNA to sensitive Spodoptera littoralis larvae. pBRJ DNA produced a typical viral infection and a comparab...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90308-e
更新日期:1990-11-01 00:00:00
abstract::Dengue virus (DENV) and West Nile virus (WNV) are members of the Flavivirus genus of positive-strand RNA viruses. RNA sequences and structures, primarily in the untranslated regions, have been shown to modulate flaviviral gene expression and genome replication. Previously, we demonstrated that a structure in the DENV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.034
更新日期:2008-09-30 00:00:00
abstract::The measles virus (MV) phosphoprotein (P) and V proteins block the interferon (IFN) response by impeding phosphorylation of the signal transducer and activator of transcription 1 (STAT1) by the Janus kinase 1 (JAK1). We characterized how STAT1 mutants interact with P and JAK1 phosphorylation. Certain mutants of the li...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.06.019
更新日期:2013-09-01 00:00:00
abstract::Sequence heterogeneity of hepatitis B virus (HBV) is increasingly recognized to play a role in virus-host interaction. We have used a recently established method for HBV full-length genome amplification to search for novel types of HBV variants and to investigate further the sequence heterogeneity of HBV genome popula...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8898
更新日期:1997-12-22 00:00:00
abstract::Terminally redundant linear proviral DNA of approximately 9.5 kb was the major unintegrated species recovered in the Hirt supernatant fraction of caprine synovial membrane cells infected with strain 75-G63 caprine arthritis-encephalitis virus. A physical map based on the cleavage sites of 13 restriction endonucleases ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90318-0
更新日期:1984-04-30 00:00:00
abstract::RNA interference (RNAi) is the process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of messenger RNA in animal and plant cells. In mammalian cells, RNAi can be triggered by 21-23 nucleotide duplexes of small interfering RNA (siRNA). Strategies to inhibit RNA virus multiplication based on ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.051
更新日期:2005-05-25 00:00:00
abstract::Measles virus infection causes a profound immunosuppression. The basis for this immunosuppression is not known. This immunosuppression could be due to virus acting directly on lymphoid cells, the production of an immunosuppressive viral product, or a lymphoid product. We have developed an antigen-specific T cell syste...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9186
更新日期:1998-06-20 00:00:00
abstract::We have utilized glycan microarray technology to determine the receptor binding properties of early isolates from the recent 2009 H1N1 human pandemic (pdmH1N1), and compared them to North American swine influenza isolates from the same year, as well as past seasonal H1N1 human isolates. We showed that the pdmH1N1 stra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.01.027
更新日期:2011-05-10 00:00:00
abstract::Phage PR4 contains a lipid bilayer within the phage protein capsid. The phospholipids of the bilayer are derived from those of the host. We report that phage morphogenesis selects against the unusually bulky phospholipids synthesized by Escherichia coli grown in the presence of various sugar alcohols. These data indic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1004
更新日期:1994-01-01 00:00:00
abstract::In this study we report the development and optimization of two minigenome rescue systems for Nipah virus, a member of the Paramyxoviridae family. One is mediated by the T7 RNA polymerase supplied either by a constitutively expressing cell line or by transfection of expression plasmids and is thus independent from inf...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.008
更新日期:2008-01-05 00:00:00
abstract::The Psu (Polarity suppression) protein of satellite bacteriophage P4 was first characterized as an anti-terminator of transcription termination in Escherichia coli. Psu is also a structural component of mature P4 capsids, where it is present as a decoration protein. Psu is located externally on the capsid surface, and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1307
更新日期:1993-06-01 00:00:00
abstract::Temperate coliphage P2 integrates its genome into the host chromosome upon lysogenization via a site-specific recombination event mediated by an integrase belonging to the complex family of tyrosine recombinases. The host integration site attB (BOB') is localized in the end of the cyaR gene and shares 27 nucleotides w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.05.009
更新日期:2010-09-01 00:00:00
abstract::The noncapsid protein expressed from ORF-206 of turnip yellow mosaic virus (TYMV) is autocatalytically processed by a papain-like protease, producing N-terminal 150-kDa and C-terminal 70-kDa proteins. By introducing two methionine residues near the N-terminus of the 70-kDa protein, we have obtained N-terminal amino ac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0131
更新日期:1996-03-01 00:00:00
abstract::The expression of type I murine interferon (MuIFN) genes and several other cellular genes was examined in poly rI.rC induced and Newcastle disease virus (NDV) infected mouse cells. Northern analysis of RNA from induced L cells revealed that the MuIFN-alpha s are expressed efficiently in NDV infected cells but only at ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90140-0
更新日期:1985-12-01 00:00:00
abstract::Nucleotide sequence analysis has shown previously that the genomic-length mRNA (mRNA1) of the coronavirus infectious bronchitis virus (IBV) contains two large open reading frames (ORFs), 1a and 1b, with the potential to encode polyproteins of approximately 441 and 300 kDa, respectively. We have characterized the speci...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1128
更新日期:1995-04-01 00:00:00
abstract::Genetic variation in dengue 2 isolates from various geographic areas was examined by oligonucleotide fingerprinting of the 40 S genome RNA. Oligonucleotide maps of geographically isolated and epidemiologically unrelated viruses were very distinct. Direct comparison of the oligonucleotide map of the dengue 2 prototype ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90255-6
更新日期:1983-07-30 00:00:00
abstract::The anatomy of DNA synthesized by five HSV-1 mutants previously shown to accumulate predominantly empty capsids at the nonpermissive temperature (NPT) was analyzed with Bg/II restriction digestion. At the NPT, all five generated DNA lacking termini, indicating that in the absence of packaging, viral DNA is not process...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90214-5
更新日期:1987-06-01 00:00:00
abstract::In order to study the effect of nef gene expression on viral replication in monocytic cells, we established monocytic (U937 and THP-1) cell transfectants constitutively expressing the human immunodeficiency virus type 1 nef gene. We constructed a plasmid expressing the nef gene derived from an infectious clone, NL432,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90272-q
更新日期:1992-12-01 00:00:00
abstract::Attachment of human adenoviruses (Ads) to host cells is mediated by the interaction of the fiber protein of the capsid with specific cell-surface molecules. For one of the species B adenoviruses, Ad3, the mechanism of binding to cells remains to be defined. Several previous reports have proposed CD46, CD80 or CD86 as ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.010
更新日期:2009-09-30 00:00:00
abstract::Pandemic H1N1 influenza A (H1N1pdm) elicits stronger pulmonary inflammation than previously circulating seasonal H1N1 influenza A (sH1N1), yet mechanisms of inflammatory activation in respiratory epithelial cells during H1N1pdm infection are unclear. We investigated host responses to H1N1pdm/sH1N1 infection and virus ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.09.022
更新日期:2014-01-05 00:00:00
abstract::We analyzed various mutants of tomato leaf curl virus-India to investigate the role of ORFs AV3, AV2, and coat protein (CP) in viral replication, movement, and symptom development. The results of these studies indicate that ORF AV3 does not encode a protein. Plants inoculated with infectious DNA which contained deleti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0546
更新日期:1996-10-15 00:00:00
abstract::The replication of vaccinia virus was reduced by 3 logs in cells that had been treated before and during infection with a concentration of N-(phosphonoacetyl)-L-aspartate (PALA) which lowered the UTP and CTP to 5 and 20% of controls, respectively, without affecting cell viability. The antiviral activity of PALA was re...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8735
更新日期:1997-09-15 00:00:00
abstract::Immunoelectron microscopy using an anti-VP2 monoclonal antibody complexed to colloidal gold has been used to study the mechanism of bluetongue virus (BTV) release from infected cells. Examination of the BTV-infected cell surface revealed that viruses are released both as enveloped particles, by budding through the pla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90218-3
更新日期:1989-11-01 00:00:00
abstract::Retroperitoneal fibromatosis herpesvirus (RFHV), the macaque homolog of the human rhadinovirus, Kaposi's sarcoma-associated herpesvirus (KSHV), was first identified in retroperitoneal fibromatosis (RF) tumor lesions of macaques with simian AIDS. We cloned and sequenced the ORF73 latency-associated nuclear antigen (LAN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.06.022
更新日期:2006-10-10 00:00:00
abstract::Chikungunya virus (CHIKV) is a mosquito-borne RNA virus that causes Chikungunya fever in humans. In this study, we generated two DNA-based CHIKV infectious clones derived from an Indian Ocean Lineage SL11131 strain and a prototype Ross strain. When the replication capabilities of the infectious CHIKV in various cell l...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.09.006
更新日期:2021-01-02 00:00:00
abstract::Replication of Tomato bushy stunt virus (TBSV) RNA takes place on the cytosolic membrane surface of peroxisomes in plants and in yeast, a model host. To identify the host proteins involved in assisting the peroxisomal localization of the tombusvirus p33 replication protein, we tested if p33 could bind directly to yeas...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.044
更新日期:2008-09-30 00:00:00
abstract::Serotype 4 rotavirus strains have been classified into two antigenic "subtypes" by a solid phase immune electron microscopy technique in which cross-absorbed animal polyclonal immune sera are used as the source of antibodies. The sequences of the gene encoding the outer capsid glycoprotein VP7 from a single serotype 4...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90766-i
更新日期:1992-05-01 00:00:00
abstract::The nucleotide and deduced amino acid sequence of the gene 4 of bovine rotavirus strain B223 is described. The open reading frame is predicted to encode a VP4 of 772 amino acids, shorter than described for any other rotavirus strain sequenced to date. B223 VP4 shows 70 to 73% similarity to other rotavirus VP4 proteins...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90191-q
更新日期:1992-11-01 00:00:00