Abstract:
:The anatomy of DNA synthesized by five HSV-1 mutants previously shown to accumulate predominantly empty capsids at the nonpermissive temperature (NPT) was analyzed with Bg/II restriction digestion. At the NPT, all five generated DNA lacking termini, indicating that in the absence of packaging, viral DNA is not processed to unit length. One mutant, F18, was able to process DNA made at the NPT to unit length molecules during a 6-hr period after shift to the permissive temperature. The appearance of unit length molecules correlated with the appearance of staphylococcal nuclease-resistant F18 DNA.
journal_name
Virologyjournal_title
Virologyauthors
Sherman G,Bachenheimer SLdoi
10.1016/0042-6822(87)90214-5subject
Has Abstractpub_date
1987-06-01 00:00:00pages
427-30issue
2eissn
0042-6822issn
1096-0341journal_volume
158pub_type
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