Abstract:
:Transient expression studies using a Nicotiana benthamiana suspension cell-derived protoplast system have identified a minimal sequence that is necessary and sufficient for activation of the tomato golden mosaic virus coat protein (CP) promoter by the viral TrAP protein (also called AL2). The sequence has a bipartite arrangement in which elements located between -125 to -107 and -96 to -60 from the transcription start site are both required for TrAP-mediated activation. One of the sequences (-96 to -60) also appears to interact with a repressor, as its deletion increases basal promoter activity in the absence of TrAP. That competition experiments using the -107 to -60 sequence to titrate the repressor also resulted in increased basal transcription is consistent with this idea. Thus, in a protoplast system which models mesophyll, regulation of the minimal CP promoter involves both activation and derepression by TrAP.
journal_name
Virologyjournal_title
Virologyauthors
Sunter G,Bisaro DMdoi
10.1006/viro.2002.1757subject
Has Abstractpub_date
2003-01-20 00:00:00pages
452-62issue
2eissn
0042-6822issn
1096-0341pii
S0042682202917575journal_volume
305pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Little is known concerning the function of the C-terminal nonconserved region of Sindbis virus nsP3. In this report, we created a number of in-frame deletions and duplications (from 12 to 159 residues) in this region and examined their effects on Sindbis virus replication. Sindbis RNA transcripts containing these muta...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1338
更新日期:1994-07-01 00:00:00
abstract::Murine monoclonal antibodies were developed against the protein products produced by murine C127 cells which had been transfected with a recombinant plasmid clone containing the human T-cell leukemia (lymphotropic) virus type I (HTLV-I) proviral DNA coding regions for part of env, px, and the 3' LTR. Four antibodies w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90270-9
更新日期:1986-04-15 00:00:00
abstract::The elucidation of the antigenic structure of the S glycoprotein of murine coronaviruses will provide further understanding of the complex pathogenicity of these viruses. In order to identify linear antigenic determinants, the primary structure of the S glycoprotein of murine hepatitis virus strain A59 was analyzed wi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1376
更新日期:1994-08-01 00:00:00
abstract::Ts1, a temperature-sensitive mutant of Moloney murine leukemia virus-TB (MoMuLV-TB), causes a progressive hindlimb paralytic disease in susceptible strains of mice. Previously, it has been shown that a single amino acid substitution, Val-25----Ile in gPr80env, is responsible for the temperature sensitivity, inefficien...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90438-h
更新日期:1991-10-01 00:00:00
abstract::Primate lentiviruses are composed of several distinct lineages, including human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus SIVagm. HIV-1 and HIV-2 have significant differences in the mechanisms of viral RNA encapsidation. Therefore, the RNA packaging mechanisms of SIVagm cannot be ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.030
更新日期:2007-06-20 00:00:00
abstract::Granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis from the baculoviruses Autographa californica, Rachiplusia ou, Heliothis zea, Heliothis armigera, Trichoplusia ni, and Spodoptera frugiperda. Antisera were raised against Autographa californica...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90128-2
更新日期:1980-05-01 00:00:00
abstract::The E7 oncoprotein of high risk human papillomavirus type 16 (HPV16) binds and inactivates the retinoblastoma (RB) family of proteins. Our previous studies suggested that HPV16 E7 enters the nucleus via a novel Ran-dependent pathway independent of the nuclear import receptors (Angeline, M., Merle, E., and Moroianu, J....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.09.037
更新日期:2009-01-05 00:00:00
abstract::Mouse mammary tumour virus (MMTV) encodes a viral superantigen (Sag) and a negative acting factor (Naf) which share parts of their coding sequence. Using 2-dimensional gel electrophoresis (2D-DIGE), we could show that at least 10 different cellular proteins were differentially expressed in Naf positive cells. Also, lu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.029
更新日期:2006-03-01 00:00:00
abstract::The International Human Papillomavirus (HPV) Reference Center supports quality and order in HPV research and diagnostics. Notably, the center assigns HPV type numbers to novel HPV types, maintains a reference clone repository, and issues international proficiency panels for HPV genotyping. The established HPV types, c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.04.003
更新日期:2018-06-01 00:00:00
abstract::La Crosse (LAC) virions exposed to different pHs (7.3, 6.2, and 5.4) and temperatures (37 degrees, 20 degrees, and 4 degrees) were preserved in thin layers of vitreous ice and observed by electron cryomicroscopy. Our results indicate that, at lower pH, virus particles interact with each other to form aggregates. In so...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90869-d
更新日期:1991-09-01 00:00:00
abstract::Alphaviruses are mosquito-borne viruses that cause serious human and animal diseases. Previous studies demonstrated that a determinant within the nsP1/nsP2 cleavage domain of the virulent Sindbis AR86 virus played a key role in regulating adult mouse virulence without adversely affecting viral replication. Additional ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.12.031
更新日期:2010-03-30 00:00:00
abstract::We have cloned an infectious genome of the Junonia coenia densonucleosis virus (JcDNV) into the bacterial plasmid pBR322. The viral genome could be rescued from the recombinant plasmid pBRJ by transfection of pBRJ DNA to sensitive Spodoptera littoralis larvae. pBRJ DNA produced a typical viral infection and a comparab...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90308-e
更新日期:1990-11-01 00:00:00
abstract::A mouse model system for a lethal encephalitis due to influenza has been established by stereotaxic microinjection with the recombinant R404BP strain of influenza A virus into the olfactory bulb of C57BL/6 mice. The virus infection spread selectively to neurons in nuclei of the broad areas of the brain parenchyma that...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1601
更新日期:2002-11-25 00:00:00
abstract::In vitro packaging experiments with phage P22 using artificially ligated plasmid concatemers have shown that the pac site is not necessary for DNA packaging although in vivo this initiation signal is indispensable. This indicates that the phage-coded protein gp3 also executes other important functions during phage mat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90273-9
更新日期:1985-07-30 00:00:00
abstract::The genes encoding the nucleoprotein, PB1, PB2, and PA proteins of the influenza virus strain B/Panamá/45/90 have been cloned under control of the T7 RNA polymerase promoter of plasmid pGEM-3. Transfection of the recombinant plasmids obtained into mammalian cells, which had been infected with a vaccinia virus encoding...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8682
更新日期:1997-09-01 00:00:00
abstract::The sequence of the 1491 nucleotides found at the 3' end of the genome of the highly aphid-transmissible (HAT) isolate of tobacco etch virus (TEV) has been determined. The nucleotide sequence of the capsid protein gene has been identified and compared with the corresponding region of the not-aphid-transmissible (NAT) ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90133-3
更新日期:1985-12-01 00:00:00
abstract::In response to oncogenic insults, normal human cells execute a defense response that culminates in cellular suicide, apoptosis. Normal human diploid fibroblasts expressing the human papillomavirus type 16 (HPV-16) E7 oncoprotein are predisposed to apoptosis when they are deprived of growth factors. Even though a domin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.11.008
更新日期:2004-02-05 00:00:00
abstract::We recently reported the phenomenon of retroviral insertion into a herpesvirus (Isfort et al., (1992) Proc. Natl. Acad. Sci. USA 89, 991-995). We have now isolated Marek's disease virus (MDV) clones that carry retroviral inserts. Retroviral LTR insertion has the potential to activate or inactivate herpesvirus genes le...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1018
更新日期:1993-01-01 00:00:00
abstract::Hepatitis A virus infection and growth in cultured cells is protracted, cell-type restricted, and generally not accompanied by the appearance of a cytopathic effect, with the exception of some culture-adapted strains. We demonstrate that the non-cytopathic HAV strain HM175/clone 1 can be induced to exhibit a cytopathi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.04.010
更新日期:2012-08-15 00:00:00
abstract::In previous studies, a mutant (cp-RSV) of the RSV A2 strain derived from 52 serial cold passages in bovine embryonic tissue culture was highly attenuated in seropositive adults and children but caused upper respiratory tract disease in seronegative infants. We investigated the genetic basis for this attenuation phenot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1178
更新日期:1995-04-20 00:00:00
abstract::The enteric adenovirus type 40 strain Dugan (Ad40) cannot be passaged in HeLa cells, but will grow in 293 cells, which express Ad5 E1 functions. To determine the reason for this limited host range, KB cell lines expressing Ad2 E1A, E1B, or E1A + E1B (L. E. Babiss, C. S. H. Young, P. B. Fisher, and H. S. Ginsberg, 1983...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90634-x
更新日期:1989-08-01 00:00:00
abstract::Polyribosomal RNA from tobacco infected with sonchus yellow net virus (SYNV) contained sequences which hybridized to 125I-labeled SYNV RNA and which were complementary to 80 to 100% of the viral RNA genome. The poly(A)-containing RNA from polyribosomes was complementary to over 90% of the viral genome but the polyribo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90375-1
更新日期:1979-08-01 00:00:00
abstract::Commelina yellow mottle virus (CoYMV) is the type member of the badnaviruses, a genus of plant pararetroviruses. The N-terminus of the polyprotein encoded by ORF III has limited similarity to known cell-to-cell movement proteins. To test the hypothesis that the N-terminus is required for viral movement, the phenotypes...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8569
更新日期:1997-06-09 00:00:00
abstract::Following primary ocular infection, HSV-1 establishes a latent infection in the trigeminal ganglia with the complete absence of detectable infectious virus. Recently, we showed that vaccination of BALB/cJ with HSV-1 glycoprotein K (gK), but not other HSV-1 glycoproteins, significantly exacerbated the severity of HSV-1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0537
更新日期:1996-10-01 00:00:00
abstract::To elucidate the virulence factors of equine herpesvirus-1 (EHV-1), we compared the genomic architecture of the HH1 strain, a Japanese isolate of EHV-1, with that of its four descendant strains obtained by serial passage in bovine kidney (BK) cells, i.e. BK77, BK161, BK271, and BK343. In preliminary studies high-passa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1228
更新日期:1994-05-01 00:00:00
abstract::In this paper we present a technique developed to physically locate the HSV-1 gene(s) which restore neurovirulence to a non-neurovirulent HSV intertypic recombinant described in the preceding report. In brief, tissue culture cells are co-transfected with unit length RE6 DNA and restriction endonuclease fragmented HSV-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90544-5
更新日期:1983-11-01 00:00:00
abstract::Tobacco (Nicotiana edwardsonii and N. benthamiana) protoplasts infected with the plant rhabdovirus sonchus yellow net virus (SYNV) were found to be suitable for studies of replication. SYNV messenger RNAs could be detected within 2 hr postinoculation (PI), accumulated to a maximum within 24 hr, and subsequently declin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90149-l
更新日期:1990-12-01 00:00:00
abstract::Two mutant strains of cucumber mosaic virus (CMV) were investigated with respect to virion stability and molecular determinants of aphid vector transmission. The mutant 2A1-MT-60x, derived from the mechanically passaged wild type 2A1-AT, is poorly transmissible by the aphid Aphis gossypii and not transmissible by the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0569
更新日期:2000-10-25 00:00:00
abstract::High Plains wheat mosaic virus (HPWMoV, genus Emaravirus; family Fimoviridae), transmitted by the wheat curl mite (Aceria tosichella Keifer), harbors a monocistronic octapartite single-stranded negative-sense RNA genome. In this study, putative proteins encoded by HPWMoV genomic RNAs 2-8 were screened for potential RN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.02.013
更新日期:2018-05-01 00:00:00
abstract::Many viruses activate the phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway, thereby modulating diverse downstream signaling pathways associated with antiapoptosis, proliferation, cell cycling, protein synthesis and glucose metabolism, in order to augment their replication. To date, the role of the PI3K-Akt p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.007
更新日期:2009-08-15 00:00:00