当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > The three subunits of the polymerase and the nucleoprotein of influenza B virus are the minimum set of viral proteins required for expression of a model RNA template.

The three subunits of the polymerase and the nucleoprotein of influenza B virus are the minimum set of viral proteins required for expression of a model RNA template.

Abstract：

:The genes encoding the nucleoprotein, PB1, PB2, and PA proteins of the influenza virus strain B/Panamá/45/90 have been cloned under control of the T7 RNA polymerase promoter of plasmid pGEM-3. Transfection of the recombinant plasmids obtained into mammalian cells, which had been infected with a vaccinia virus encoding the T7 RNA polymerase, resulted in expression of the expected influenza B virus polypeptides. Moreover, it is shown that coexpression of the four recombinant core proteins in COS-1 cells reconstituted a functional polymerase capable of expressing a synthetic influenza B virus-like CAT RNA. By using the influenza B virus recombinant plasmids and a set of pGEM-derived plasmids encoding the homologous core proteins of the influenza A virus A/Victoria/3/75 (I. Mena et al. (1994). J. Gen. Virol. 75, 2109-2114), the capabilities of homo- and heterotypic mixtures of the four core proteins to express synthetic type A and B CAT RNAs were analyzed. Both the influenza A and B virus polymerases were active in expressing, albeit with reduced efficiencies, the heterotypic model CAT RNAs. However, none of all possible heterotypic mixtures of the core proteins reconstituted a functional polymerase. In order to fully characterize the recombinant plasmids obtained, the nucleotide sequences of the cloned genes were determined and compared to sequences of other type B virus isolates. The results obtained from these latter analyses are discussed in terms of the conservation and evolution of the influenza B virus core genes.

journal_name

Virology

journal_title

Virology

authors

Jambrina E,Bárcena J,Uez O,Portela A

doi

10.1006/viro.1997.8682

subject

Has Abstract

pub_date

1997-09-01 00:00:00

pages

209-17

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042682297986827

journal_volume

235

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Epidermal growth factor receptor is a co-factor for transmissible gastroenteritis virus entry.

    abstract：:Transmissible gastroenteritis virus (TGEV) causes severe diarrhea and high mortality in newborn piglets. It is well established that porcine intestinal epithelium is the target of the TGEV infection, however the mechanism that TGEV invades the host epithelium remains largely unknown. Aminopeptidase N (APN) is a known ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2018.05.009

    authors： Hu W,Zhang S,Shen Y,Yang Q

    更新日期：2018-08-01 00:00:00

  • A new hepatitis B virus variant in a chronic carrier with multiple episodes of viral reactivation and acute hepatitis.

    abstract：:To examine whether there are HBV variants not yet described which cannot express HBe due to a mutation in the pre-C region, and, if they exist, whether they cause a particular course of infection and disease, we analyzed the HBV genomes of a HBs/anti-HBe positive chronic carrier who had several episodes of acute reexa...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90274-u

    authors： Raimondo G,Schneider R,Stemler M,Smedile V,Rodino G,Will H

    更新日期：1990-11-01 00:00:00

  • Conserved amino acids of the human immunodeficiency virus type 2 Vpx nuclear localization signal are critical for nuclear targeting of the viral preintegration complex in non-dividing cells.

    abstract：:The HIV-2 viral accessory protein Vpx is related to, but distinct from the Vpr protein of HIV-1. Vpx is packaged into virions and as a component of the viral preintegration complex (PIC) is required for efficient virus replication in non-dividing cells. We have previously reported that the minimal transferable region ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.10.036

    authors： Belshan M,Mahnke LA,Ratner L

    更新日期：2006-03-01 00:00:00

  • Multiplicity-dependent induction of viral capsid antigen in Raji cells superinfected with Epstein-Barr virus.

    abstract：:A quantitative analysis of Epstein-Barr virus (EBV)-induced early antigen (EA) and viral capsid antigen (VCA) syntheses was carried out in Raji cells superinfected with purified, concentrated P3HR-1 EBV. When the cells were exposed to the virus and assessed by immunofluorescence and immunoprecipitation, EA induction o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90276-3

    authors： Fujiwara S,Takada K,Yano S,Osato T

    更新日期：1983-07-30 00:00:00

  • Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase.

    abstract：:The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant wer...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.04.007

    authors： Murata T,Isomura H,Yamashita Y,Toyama S,Sato Y,Nakayama S,Kudoh A,Iwahori S,Kanda T,Tsurumi T

    更新日期：2009-06-20 00:00:00

  • Differentiation of acid-pH-dependent and -nondependent entry pathways for mouse hepatitis virus.

    abstract：:Early events of infection of MHV were studied in comparison with those of VSV, which is known to enter cells by an endocytic pathway. Treatment of mouse L-2 fibroblasts with ammonium chloride, chloroquine, or dansylcadaverine inhibited infection of MHV to a much lesser degree than that of VSV, suggesting a relatively ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90014-3

    authors： Kooi C,Cervin M,Anderson R

    更新日期：1991-01-01 00:00:00

  • UL5, a protein required for HSV DNA synthesis: genetic analysis, overexpression in Escherichia coli, and generation of polyclonal antibodies.

    abstract：:The mutations in two DNA-negative ts mutants of herpes simplex virus type 1 (HSV-1), tsK13 and tsM19, have been previously mapped to a 2.0-kb fragment (coordinates 0.095-0.108) at the left end of the genome (S. Weller, D. Aschman, W. Sacks, D. Coen, and P. Schaffer, 1983, Virology 130, 290-305). Sequence analysis of t...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90507-7

    authors： Zhu L,Weller SK

    更新日期：1988-10-01 00:00:00

  • The flavivirus-conserved penta-nucleotide in the 3' stem-loop of the West Nile virus genome requires a specific sequence and structure for RNA synthesis, but not for viral translation.

    abstract：:A reporting replicon of West Nile virus (WN) was used to distinguish between the function of the 3' untranslated region (UTR) in viral translation and RNA replication. Deletions of various regions of the 3' UTR of the replicon did not significantly affect viral translation, but abolished RNA replication. A systematic ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.07.022

    authors： Tilgner M,Deas TS,Shi PY

    更新日期：2005-01-20 00:00:00

  • An improved reverse genetics system for mammalian orthoreoviruses.

    abstract：:Mammalian orthoreoviruses (reoviruses) are highly useful models for studies of double-stranded RNA virus replication and pathogenesis. We previously developed a strategy to recover prototype reovirus strain T3D from cloned cDNAs transfected into murine L929 fibroblast cells. Here, we report the development of a second...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.11.037

    authors： Kobayashi T,Ooms LS,Ikizler M,Chappell JD,Dermody TS

    更新日期：2010-03-15 00:00:00

  • Nucleotide sequence of the 26 S mRNA of the virulent Trinidad donkey strain of Venezuelan equine encephalitis virus and deduced sequence of the encoded structural proteins.

    abstract：:A cDNA clone containing all of the 26 S mRNA coding region of the RNA genome of Venezuelan equine encephalitis (VEE) virus, virulent strain Trinidad donkey (TRD), has been constructed and sequenced. The nucleotide and deduced amino acid sequences of the 26 S RNA of VEE virus conform to the general organization of the ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90142-x

    authors： Kinney RM,Johnson BJ,Brown VL,Trent DW

    更新日期：1986-07-30 00:00:00

  • Single-cell RNA sequencing reveals cell type-specific HPV expression in hyperplastic skin lesions.

    abstract：:Human Papillomavirus infection is highly prevalent worldwide. While most types of HPV cause benign warts, some high-risk types are known to cause cervical cancer, as well as cancer of the oral cavity and head and neck. Persistent cutaneous HPV infection can be particularly problematic in patients with chronic immunosu...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.08.007

    authors： Devitt K,Hanson SJ,Tuong ZK,McMeniman E,Soyer HP,Frazer IH,Lukowski SW

    更新日期：2019-11-01 00:00:00

  • A specific host cellular protein binding element near the 3' end of mouse hepatitis virus genomic RNA.

    abstract：:A distinct host cellular protein binding element was mapped within a 38-nucleotide (nt) sequence 166-129 nucleotides upstream of the 3' end of the MHV-JHM genome using a RNase T1 protection/gel mobility shift electrophoresis assay. The resultant RNA-protein complex contains six host cellular proteins, one protein of 1...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8553

    authors： Liu Q,Yu W,Leibowitz JL

    更新日期：1997-05-26 00:00:00

  • Subnuclear localization and phosphorylation of Epstein-Barr virus latent infection nuclear proteins.

    abstract：:Functions of the six Epstein-Barr virus latent infection nuclear proteins (EBNA-1, -2, -3A, -3B, -3C, or -LP) in maintaining latent infection or cell growth transformation are only partially understood. Using antibodies specific for each EBNA in immunofluorescence microscopy, EBNA-2, -3A, and -3C localized to subnucle...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90027-o

    authors： Petti L,Sample C,Kieff E

    更新日期：1990-06-01 00:00:00

  • Different evolution of simian immunodeficiency virus in a natural host and a new host.

    abstract：:To address the mechanisms of host-virus adaptation and pathogenesis of lentiviral infections, we compared the evolution of the same isolate of simian immunodeficiency virus (SIVsmm9) in two different situations: nonpathogenic infection of its natural host, the sooty mangabey, and AIDS-inducing infection of a new host,...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9217

    authors： Courgnaud V,Saurin W,Villinger F,Sonigo P

    更新日期：1998-07-20 00:00:00

  • The HIV-1 protein Vpr targets the endoribonuclease Dicer for proteasomal degradation to boost macrophage infection.

    abstract：:The HIV-1 protein Vpr enhances macrophage infection, triggers G2 cell cycle arrest, and targets cells for NK-cell killing. Vpr acts through the CRL4(DCAF1) ubiquitin ligase complex to cause G2 arrest and trigger expression of NK ligands. Corresponding ubiquitination targets have not been identified. UNG2 and SMUG1 are...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.06.010

    authors： Casey Klockow L,Sharifi HJ,Wen X,Flagg M,Furuya AK,Nekorchuk M,de Noronha CM

    更新日期：2013-09-01 00:00:00

  • Select resistance-associated mutations in blood are associated with lower CSF viral loads and better neuropsychological performance.

    abstract：BACKGROUND:When antiretroviral therapy does not fully suppress HIV replication, suboptimal levels of antiretrovirals can select for antiretroviral resistant variants of HIV. These variants may exhibit reduced replication capacity and result in lower viral loads in blood. Our study evaluated whether antiretroviral resis...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.08.007

    authors： Hightower GK,Letendre SL,Cherner M,Gibson SA,Ellis RJ,Wolfson TJ,Gamst AC,Ignacio CC,Heaton RK,Grant I,Richman DD,Smith DM,HNRC Group.

    更新日期：2009-11-25 00:00:00

  • Adenovirus infection inactivates the translational inhibitors 4E-BP1 and 4E-BP2.

    abstract：:Infection with many viruses results in the selective shutoff of host protein synthesis. A common target for virus interference with host protein synthesis is the cap-binding protein complex, eIF4F. The large subunit of the complex, eIF4G, is cleaved upon picornavirus (except cardiovirus) infection. Infection with aden...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8757

    authors： Gingras AC,Sonenberg N

    更新日期：1997-10-13 00:00:00

  • Inhibition of VSV genome RNA replication but not transcription by monoclonal antibodies specific for the viral P protein.

    abstract：:The growth of vesicular stomatitis virus requires two distinct RNA synthetic events: transcription of messenger RNA molecules and replication of the viral genome RNA. We report the use of a panel of monoclonal antibodies directed against the viral phosphoprotein P in an attempt to assess the role of this protein in RN...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0031

    authors： Richardson JC,Peluso RW

    更新日期：1996-02-01 00:00:00

  • Selective inhibition of protein synthesis by synthetic and vaccinia virus-core synthesized poly(riboadenylic acids).

    abstract：:Studies were undertaken to compare the effect of poly(A)s from various sources on selective inhibition of protein synthesis in the reticulocyte lysate system programmed with viral and cellular mRNAs. RNA synthesized in vitro by vaccinia virus cores in the presence of only ATP inhibited overall HeLa cell polypeptide sy...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90129-2

    authors： Bablanian R,Goswami SK,Esteban M,Banerjee AK

    更新日期：1987-12-01 00:00:00

  • Novel DNA virus isolated from samples showing endothelial cell necrosis in the Japanese eel, Anguilla japonica.

    abstract：:Economic loss due to viral endothelial cell necrosis of eel (VECNE) of Anguilla japonica is a serious problem for the cultured Japanese eel market. However, the viral genome responsible for VECNE is unknown. We recently developed a rapid determination system for viral nucleic acid sequences (RDV) to determine viral ge...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.12.057

    authors： Mizutani T,Sayama Y,Nakanishi A,Ochiai H,Sakai K,Wakabayashi K,Tanaka N,Miura E,Oba M,Kurane I,Saijo M,Morikawa S,Ono S

    更新日期：2011-03-30 00:00:00

  • Membrane topology of the vaccinia virus A17L envelope protein.

    abstract：:The formation of a lipoprotein membrane within specialized areas of the cytoplasm is the first visible step in poxvirus morphogenesis. The A17L viral protein, an essential nonglycosylated membrane component, was predicted to have four centrally located alpha-helical membrane-spanning domains. The gene was expressed as...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.9870

    authors： Betakova T,Wolffe EJ,Moss B

    更新日期：1999-09-01 00:00:00

  • Viral cytopathogenicity correlated with integration of ubiquitin-coding sequences.

    abstract：:The RNA genomes of cytopathogenic bovine viral diarrhea virus (BVDV) isolates contain insertions highly homologous to cellular sequences. For two of them the insert was identified as ubiquitin coding sequence. The genome of BVDV Osloss contains exactly one ubiquitin gene monomer. In the case of BVDV CP1 the cellular i...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90074-l

    authors： Meyers G,Tautz N,Dubovi EJ,Thiel HJ

    更新日期：1991-02-01 00:00:00

  • A novel type of VP4 carried by a porcine rotavirus strain.

    abstract：:The gene encoding the VP8* trypsin-cleavage product of the VP4 protein of porcine rotavirus strain A34 was sequenced, and the predicted amino acid (aa) sequence was compared to the homologous region of all known P genotypes. The aa sequence of the VP8* of strain A34 shared low identity, ranging from 39% (bovine strain...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00534-8

    authors： Liprandi F,Gerder M,Bastidas Z,López JA,Pujol FH,Ludert JE,Joelsson DB,Ciarlet M

    更新日期：2003-10-25 00:00:00

  • Complementation analysis of the dales collection of vaccinia virus temperature-sensitive mutants.

    abstract：:A collection of randomly generated temperature-sensitive (ts) vaccinia virus (strain IHD-W) mutants were reported by S. Dales et al., (1978, Virology, 84, 403-428) in 1978 and characterized by electron microscopy. We have performed further genetic analysis on the Dales collection of mutants to make the mutants more us...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2002.1745

    authors： Lackner CA,D'Costa SM,Buck C,Condit RC

    更新日期：2003-01-20 00:00:00

  • Antigenic analyses of influenza virus haemagglutinins with different receptor-binding specificities.

    abstract：:Monoclonal antibodies were used to compare the antigenicities of the haemagglutinins of two receptor binding mutants of X-31 (H3N2) influenza virus. The mutants which differed from each other in recognizing sialic acid in either alpha 2-6 linkage or alpha 2-3 linkage to galactose also differed exclusively at residue 2...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(84)90158-2

    authors： Daniels RS,Douglas AR,Skehel JJ,Wiley DC,Naeve CW,Webster RG,Rogers GN,Paulson JC

    更新日期：1984-10-15 00:00:00

  • Use of chimeric nectin-1(HveC)-related receptors to demonstrate that ability to bind alphaherpesvirus gD is not necessarily sufficient for viral entry.

    abstract：:Human nectin-1 (HveC, Prr1), a member of the immunoglobulin superfamily and a receptor for the entry of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1), binds to viral gD. For HSV-1, HSV-2, and PRV, the gD-binding region of nectin-1 has been localized to the N-...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.0989

    authors： Geraghty RJ,Fridberg A,Krummenacher C,Cohen GH,Eisenberg RJ,Spear PG

    更新日期：2001-07-05 00:00:00

  • Biology of the temperate Streptococcus thermophilus bacteriophage TP-J34 and physical characterization of the phage genome.

    abstract：:The temperate Streptococcus thermophilus bacteriophage TP-J34 was identified in the lysogenic host strain J34. The majority of phage particles produced upon induction was defective and noninfectious, consisting of DNA-filled heads lacking tails. A physical map (45.6 kb) was established. Analysis of minor restriction b...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00516-6

    authors： Neve H,Freudenberg W,Diestel-Feddersen F,Ehlert R,Heller KJ

    更新日期：2003-10-10 00:00:00

  • Differential effect of poly rI.rC and Newcastle disease virus on the expression of interferon and cellular genes in mouse cells.

    abstract：:The expression of type I murine interferon (MuIFN) genes and several other cellular genes was examined in poly rI.rC induced and Newcastle disease virus (NDV) infected mouse cells. Northern analysis of RNA from induced L cells revealed that the MuIFN-alpha s are expressed efficiently in NDV infected cells but only at ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90140-0

    authors： Kelley KA,Pitha PM

    更新日期：1985-12-01 00:00:00

  • In vitro excision and replication of 5' telomeres of minute virus of mice DNA from cloned palindromic concatemer junctions.

    abstract：:HeLa cell extracts containing wild-type copies of the minute virus of mice NS-1 polypeptide produced from a recombinant vaccinia virus vector could support the excision and replication of viral 5' telomeres from cloned concatemer junction fragments. Resolution did not occur if wild-type NS-1 was omitted from the extra...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)91223-h

    authors： Cotmore SF,Nuesch JP,Tattersall P

    更新日期：1992-09-01 00:00:00

  • Induction of apoptosis by the Vpr protein of human immunodeficiency virus type 1 occurs independently of G(2) arrest of the cell cycle.

    abstract：:The HIV-1 accessory gene product Vpr can inhibit cell proliferation via cell cycle arrest at the G(2) phase, and it can induce apoptosis after G(2) arrest. We found recently that C81, a carboxy-terminally truncated form of Vpr, induced apoptosis via G(1) arrest but did not induce G(2) arrest of the cell cycle. Thus, i...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0534

    authors： Nishizawa M,Kamata M,Mojin T,Nakai Y,Aida Y

    更新日期：2000-10-10 00:00:00