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Single-cell RNA sequencing reveals cell type-specific HPV expression in hyperplastic skin lesions.

Abstract：

:Human Papillomavirus infection is highly prevalent worldwide. While most types of HPV cause benign warts, some high-risk types are known to cause cervical cancer, as well as cancer of the oral cavity and head and neck. Persistent cutaneous HPV infection can be particularly problematic in patients with chronic immunosuppression, for example following organ transplantation. Due to unknown mechanisms, these patients may develop numerous warts, as well as present with a dramatically increased skin cancer prevalence. Despite an association between HPV persistence in the epidermis and excessive wart or squamous cancer development, the molecular mechanisms linking immunosuppression, HPV expression and excessive epidermal proliferation have not been determined, largely due to low-sensitivity methodology to capture rare viral transcription events. Here, we use single-cell RNA sequencing to profile HPV-positive skin lesions from an immunosuppressed patient that were found to express the alphapapillomavirus HPV78 in basal keratinocytes, suprabasal keratinocytes and hair follicle stem cells. This method can be applied to detect and investigate HPV transcripts in cutaneous lesions, allowing mechanistic links between immunosuppression-induced HPV life cycle and epidermal hyperproliferation to be uncovered.

journal_name

Virology

journal_title

Virology

authors

Devitt K,Hanson SJ,Tuong ZK,McMeniman E,Soyer HP,Frazer IH,Lukowski SW

doi

10.1016/j.virol.2019.08.007

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

14-19

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(19)30220-X

journal_volume

537

pub_type

杂志文章

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