Retrovirus insertion into herpesvirus: characterization of a Marek's disease virus harboring a solo LTR.

abstract：:In order to study the effect of nef gene expression on viral replication in monocytic cells, we established monocytic (U937 and THP-1) cell transfectants constitutively expressing the human immunodeficiency virus type 1 nef gene. We constructed a plasmid expressing the nef gene derived from an infectious clone, NL432,...

journal_title：Virology

authors： Tsunetsugu-Yokota Y,Matsuda S,Maekawa M,Saito T,Takemori T,Takebe Y

更新日期：1992-12-01 00:00:00

abstract：:Lloviu virus (LLOV), a phylogenetically divergent filovirus, is the proposed etiologic agent of die-offs of Schreibers's long-fingered bats (Miniopterus schreibersii) in western Europe. Studies of LLOV remain limited because the infectious agent has not yet been isolated. Here, we generated a recombinant vesicular sto...

journal_title：Virology

authors： Ng M,Ndungo E,Jangra RK,Cai Y,Postnikova E,Radoshitzky SR,Dye JM,Ramírez de Arellano E,Negredo A,Palacios G,Kuhn JH,Chandran K

更新日期：2014-11-01 00:00:00

abstract：:Human immunodeficiency virus (HIV) Gag proteins are assembled into virus particles and then cleaved by the virion-associated HIV protease. Concomitant with Gag processing, doughnut-like HIV particles (the immature form) are converted to particles containing condensed cores (the mature form). Here we describe the in vi...

journal_title：Virology

authors： Morikawa Y,Shibuya M,Goto T,Sano K

更新日期：2000-07-05 00:00:00

abstract：:Our previous studies have demonstrated that virus-specific cell-mediated cytotoxicity of sensitized leukocytes can be induced using clonal ginbuna crucian carp and their syngeneic cell lines. In the present study, we attempt to determine if virus-specific cytotoxic cell populations of fish express CD8alpha and TCRbeta...

journal_title：Virology

authors： Somamoto T,Yoshiura Y,Sato A,Nakao M,Nakanishi T,Okamoto N,Ototake M

更新日期：2006-05-10 00:00:00

abstract：:To better understand the evolution of the viral envelope glycoproteins (Env) in HIV-1 infected individuals who progress to AIDS maintaining an exclusive CCR5-using (R5) virus population, we cloned and sequenced the env gene of longitudinally obtained primary isolates. A shift in the electrostatic potential towards an ...

journal_title：Virology

authors： Repits J,Sterjovski J,Badia-Martinez D,Mild M,Gray L,Churchill MJ,Purcell DF,Karlsson A,Albert J,Fenyö EM,Achour A,Gorry PR,Jansson M

更新日期：2008-09-15 00:00:00

abstract：:The repetitive DNA sequences of the genome of Molluscum contagiosum virus type 1 (MCV-1) have been localized within the terminal regions of the viral genome corresponding to the BamHI MCV-1 DNA fragments B (18 kbp; 0 to 0.095 map units (m.u.)) and E (10.5 kbp; 0.944 to 1 m.u.). The fine mapping of these particular reg...

journal_title：Virology

authors： Bugert JJ,Raab K,Rösen-Wolff A,Janssen W,Darai G

更新日期：1993-01-01 00:00:00

abstract：:Pandemic H1N1 influenza A (H1N1pdm) elicits stronger pulmonary inflammation than previously circulating seasonal H1N1 influenza A (sH1N1), yet mechanisms of inflammatory activation in respiratory epithelial cells during H1N1pdm infection are unclear. We investigated host responses to H1N1pdm/sH1N1 infection and virus ...

journal_title：Virology

authors： Paquette SG,Banner D,Chi le TB,Leόn AJ,Xu L,Ran L,Huang SS,Farooqui A,Kelvin DJ,Kelvin AA

更新日期：2014-01-05 00:00:00

abstract：:The replicative form (RF) of spiroplasma virus 4 (SpV4) has been purified from infected cells of Spiroplasma melliferum strain G1 by alkaline lysis followed by low melting point agarose gel electrophoresis. A partial restriction map has been established. The circular RF was linearized by cutting at the unique ClaI res...

journal_title：Virology

authors： Pascarel-Devilder MC,Renaudin J,Bove JM

更新日期：1986-06-01 00:00:00

abstract：:Monoclonal antibodies directed against glycoprotein D of herpes simplex virus completely inhibited fusion of Vero cells infected with type 1 virus. In contrast, several monoclonal antibodies directed against other viral glycoproteins, including B, were ineffective or were only minimally inhibitory at the highest conce...

journal_title：Virology

authors： Noble AG,Lee GT,Sprague R,Parish ML,Spear PG

更新日期：1983-08-01 00:00:00

abstract：:To investigate the mode of gene expression of red clover mottle virus (RCMV) middle component (M) RNA, we have synthesized an oligopeptide corresponding to the predicted carboxy-terminus of the RCMV counterparts of the cowpea mosaic virus (CPMV) 48K and 58K proteins. Using an antiserum raised against this synthetic ol...

journal_title：Virology

authors： Shanks M,Tomenius K,Clapham D,Huskisson NS,Barker PJ,Wilson IG,Maule AJ,Lomonossoff GP

更新日期：1989-12-01 00:00:00

abstract：:Most adenovirus transducing vectors have the cytomegalovirus major immediate-early (CMV) or the Rous sarcoma virus long terminal repeat (RSV) promoter driving expression of the transgene. Both of these promoters are highly active in transfection and transduction assays in 293 cells, in which transducing vectors are co...

journal_title：Virology

authors： Schaack J,Allen B,Orlicky DJ,Bennett ML,Maxwell IH,Smith RL

更新日期：2001-12-05 00:00:00

abstract：:We assess the genetic relationships between 49 HIV-1 group O strains from 24 and 25 patients living in Cameroon and France, respectively. Strains were sequenced in four genomic regions: gag (p24) and three env regions (C2-V3, gp41, and for 22 C2-gp41). In each of the genomic regions analyzed, the genetic diversity amo...

journal_title：Virology

authors： Roques P,Robertson DL,Souquière S,Damond F,Ayouba A,Farfara I,Depienne C,Nerrienet E,Dormont D,Brun-Vézinet F,Simon F,Mauclère P

更新日期：2002-10-25 00:00:00

abstract：:We report here the nonenzymatic self-ligation of transcripts corresponding to the peach latent mosaic viroid (PLMVd). This is the first description of this process with viroid sequences, although it has been reported to occur with human hepatitis delta virus RNA. Self-ligation occurs when the 5'-hydroxyl and the 2',3'...

journal_title：Virology

authors： Lafontaine D,Beaudry D,Marquis P,Perreault JP

更新日期：1995-10-01 00:00:00

abstract：:Using a defective interfering Sendai virus stock (DIH4) freed of nondefective helper virus, we found that the closely related parainfluenza viruses 1 and 3 could substitute for the Sendai virus helper in replicating DIH4, creating chimeric nucleocapsids. The morbillivirus measles and the rhabdovirus VSV could not subs...

journal_title：Virology

authors： Curran JA,Kolakofsky D

更新日期：1991-05-01 00:00:00

abstract：:The Nef protein of Simian immunodeficiency virus (SIV) associates with multiple T lymphocyte signaling proteins, including the T cell receptor (TCR) zeta chain. We demonstrate here that these interactions are conserved and highly specific. Nefs derived from genetically diverse strains of SIV (SIV(mac)239, SIV(smm)PBj,...

journal_title：Virology

authors： Schaefer TM,Bell I,Pfeifer ME,Ghosh M,Trible RP,Fuller CL,Ashman C,Reinhart TA

更新日期：2002-10-10 00:00:00

abstract：:Assembly of the viral genome into virions is a critical process of the virus life cycle often defining the ability of the virus to move within the plant and to be transmitted horizontally to other plants. Closteroviridae virions are polar helical rods assembled primarily by a major coat protein, but with a related min...

journal_title：Virology

authors： Satyanarayana T,Gowda S,Mawassi M,Albiach-Martí MR,Ayllón MA,Robertson C,Garnsey SM,Dawson WO

更新日期：2000-12-05 00:00:00

abstract：:The NIa protein of potyviruses provides VPg and proteolytic functions during virus replication. It has also been shown to confer host genotype-specific movement functions in plants. Specifically, NIa from tobacco etch virus (TEV)-Oxnard, but not from most other strains, confers the ability to move long distances in Ni...

journal_title：Virology

authors： Schaad MC,Anderberg RJ,Carrington JC

更新日期：2000-08-01 00:00:00

abstract：:Piglets infected in utero with porcine reproductive and respiratory syndrome virus (PRRSV) are born severely immunocompromised. In this article we more closely examine the effects of in utero PRRSV infection on circulating and thymic T cell populations. Numbers of CD4+, CD8+, and dual-positive lymphocytes were quantit...

journal_title：Virology

authors： Feng WH,Tompkins MB,Xu JS,Brown TT,Laster SM,Zhang HX,McCaw MB

更新日期：2002-10-25 00:00:00

abstract：:To determine whether infection with HIV-1 strains of different tropisms would influence expression of the mucosa-associated integrins alpha 4 beta 7 and alpha E beta 7 or the lymph node homing receptor L-selectin on peripheral T lymphocytes, cells were infected with the CXCR4-tropic (X4)/syncytium-inducing (SI) HIV-1(...

journal_title：Virology

authors： Cummins JE Jr,Bunn WJ,Hall SD,Donze HH,Mestecky J,Jackson S

更新日期：2001-02-15 00:00:00

abstract：:The roles of two porcine circovirus replication initiator proteins, Rep and Rep׳, in generating copy-release and rolling-circle DNA replication intermediates were determined. Rep uses the supercoiled closed-circular genome (ccc) to initiate leading-strand synthesis (identical to copy-release replication) and generates...

journal_title：Virology

authors： Cheung AK

更新日期：2015-07-01 00:00:00

abstract：:We describe the in vitro selection and characterisation of virus derived from B/Beijing/1/87 passaged in the presence of zanamivir. During zanamivir passage, the phenotype of virus isolates was either drug dependent or drug resistant in plaque reduction assays. The susceptibility of the neuraminidase of the drug-depen...

journal_title：Virology

authors： Barnett JM,Cadman A,Burrell FM,Madar SH,Lewis AP,Tisdale M,Bethell R

更新日期：1999-12-20 00:00:00

abstract：:Herpes simplex viruses encode several glycoproteins dispensable for infection and replication in cell culture. Evidence is presented that there exist at least two pathways for viral attachment to cells, i.e., one mediated by the dispensable glycoprotein C (gC) and one independent of that glycoprotein. Thus, whereas th...

journal_title：Virology

authors： Campadelli-Fiume G,Stirpe D,Boscaro A,Avitabile E,Foá-Tomasi L,Barker D,Roizman B

更新日期：1990-09-01 00:00:00

abstract：:The complete nucleotide sequence of the RSV cpts-248/404 live attenuated vaccine candidate was determined from cloned cDNA and was compared to that of the RSV A2/HEK7 wild-type, cold-passaged cp-RSV, and cpts-248 virus, which constitute the series of progenitor viruses. RSV cpts-248/404 is more attenuated and more tem...

journal_title：Virology

authors： Firestone CY,Whitehead SS,Collins PL,Murphy BR,Crowe JE Jr

更新日期：1996-11-15 00:00:00

abstract：:Herpes simplex virus mutant strains that fail to encode a functional ICP27 polypeptide show a reduction in their ability to replicate viral DNA compared to wild-type virus. To define the nature of this defect, we examined the formation of viral DNA replication structures in the nucleus of cells infected with these mut...

journal_title：Virology

authors： Curtin KD,Knipe DM

更新日期：1993-09-01 00:00:00

abstract：:We have previously described a selective increase in HIV-DNA content in CCR5-negative lymphocytes from late stage HIV-infected patients. Here, we show that this increase occurred even in the absence of viral phenotypic switching from CCR5- to CXCR4-tropic. This leads us to hypothesize that early and late CCR5-tropic v...

journal_title：Virology

authors： Pakarasang M,Wasi C,Suwanagool S,Chalermchockcharoenkit A,Auewarakul P

更新日期：2006-04-10 00:00:00

abstract：:Seven envelope regions from two series of patient isolates have been molecularly cloned and analyzed for replication phenotypes and immunogenicity. Growth potential was analyzed for env sequences substituted into an HIV-1-NL4-3 backbone (NL4-3/env recombinants). Immunogenicity studies were conducted on secreted monome...

journal_title：Virology

authors： Mustafa F,Richmond JF,Fernandez-Larsson R,Lu S,Fredriksson R,Fenyö EM,O'Connell M,Johnson E,Weng J,Santoro JC,Robinson HL

更新日期：1997-03-03 00:00:00

abstract：:The adenovirus (Ad) single-stranded DNA binding protein (DBP) is a multifunctional protein. It is thought to consist of two domains, the amino-terminal domain involved in host-range determination and the carboxyl-terminal domain functioning in DNA replication and DNA binding. We have determined the nucleotide sequence...

journal_title：Virology

authors： Vos HL,van der Lee FM,Reemst AM,van Loon AE,Sussenbach JS

更新日期：1988-03-01 00:00:00

abstract：:Thirteen temperature-sensitive (ts) mutants of human cytomegalovirus (HCMV) have been isolated after mutagenesis with nitrosoguanidine or ultraviolet light. Four mutants defective in viral DNA synthesis at nonpermissive temperature (DNA-) were classified into two separate complementation groups. The other nine HCMV ts...

journal_title：Virology

authors： Dion M,Hamelin C

更新日期：1987-05-01 00:00:00

abstract：:Rotaviral RNA was detected in the stool sample of an asymptomatic fattening pig at a Slovenian pig farm. To characterize the rotavirus, RT-PCR was used, employing primers specific for the VP7, VP4 and NSP4 genes. Specific products were purified and the sequencing reaction was performed for the molecular analysis of am...

journal_title：Virology

authors： Steyer A,Poljsak-Prijatelj M,Barlic-Maganja D,Jamnikar U,Mijovski JZ,Marin J

更新日期：2007-03-15 00:00:00

abstract：:Determinants of temperature sensitivity and/or attenuation in Sabin type 1 poliovirus reside in the 5' NTR and coding sequences of the capsid proteins and viral RNA polymerase, 3D(pol). Previous studies have implicated at least two mutations in 3D(pol) of Sabin 1 vaccine strain [PV1(S)], including a Y73H change, as co...

journal_title：Virology

authors： Paul AV,Mugavero J,Yin J,Hobson S,Schultz S,van Boom JH,Wimmer E

更新日期：2000-06-20 00:00:00