Molecular characterization of a new porcine rotavirus P genotype found in an asymptomatic pig in Slovenia.

abstract：:The minute virus of mice, prototype strain (MVMp), is a non-enveloped, single-stranded DNA virus of the family Parvoviridae. Unlike other parvoviruses, the mechanism of cellular uptake of MVMp has not been studied in detail. We analyzed MVMp endocytosis in mouse LA9 fibroblasts and a tumor cell line derived from epith...

journal_title：Virology

authors： Garcin PO,Panté N

更新日期：2015-08-01 00:00:00

abstract：:Identification of regulators of p53 expression is a crucial step in understanding the diverse functions of p53 and its role in cellular homeostasis and responsiveness to insult. Several viral proteins inactivate p53 as a modulator of cell cycle progression and apoptosis. Here, we report that a unique leporipoxviral tr...

journal_title：Virology

authors： Wali A,Strayer DS

更新日期：1996-10-01 00:00:00

abstract：:Murine gammaherpesvirus 68 (MHV-68) replicates robustly in cell culture, providing a model for studying viral genome replication during de novo infection of tumor-associated herpesviruses. We have previously identified a 1.25-kb origin of lytic replication (oriLyt) for MHV-68. To further investigate the molecular mech...

journal_title：Virology

authors： Gong D,Qi J,Arumugaswami V,Sun R,Deng H

更新日期：2009-05-10 00:00:00

abstract：:nsP3 is one of four viral nonstructural proteins required for RNA replication of Sindbis virus. In this report, post-translational modifications of nsP3 which occur in both vertebrate and mosquito cell cultures have been examined. In pulse-chase experiments, analyzed by immunoprecipitation and sodium dodecyl sulfate-p...

journal_title：Virology

authors： Li GP,La Starza MW,Hardy WR,Strauss JH,Rice CM

更新日期：1990-11-01 00:00:00

abstract：:The N-terminal portion of the reovirus cell attachment protein sigma 1 has recently been shown to possess intrinsic oligomerization and virion-anchoring functions. Sequence analysis of the sigma 1 proteins of the three reovirus serotypes has revealed the presence of distinct structural domains within this region: a te...

journal_title：Virology

authors： Leone G,Mah DC,Lee PW

更新日期：1991-05-01 00:00:00

abstract：:The single-stranded DNA-binding protein LEF-3 of Autographa californica multinucleocapsid nucleopolyhedrovirus consists of 385 amino acid residues, forms oligomers, and promotes Mg2+-independent unwinding of DNA duplexes and annealing of complementary DNA strands. Partial proteolysis revealed that the DNA-binding doma...

journal_title：Virology

authors： Mikhailov VS,Okano K,Rohrmann GF

更新日期：2006-03-15 00:00:00

abstract：:The orthopoxvirus serpin SPI-3 is N-glycosylated and suppresses fusion between infected cells. Although SPI-3 contains motifs conserved in inhibitory serpins, no proteinase inhibition by SPI-3 has been demonstrated, and mutations within the serpin reactive center loop (RCL) do not affect the ability to regulate cell f...

journal_title：Virology

authors： Turner PC,Baquero MT,Yuan S,Thoennes SR,Moyer RW

更新日期：2000-07-05 00:00:00

abstract：:As porcine endogenous retroviruses (PERV) productively infect human cells in vitro, they pose a serious risk in xenotransplantation and xenogeneic cell therapies. We have analyzed the prevalence of six well-characterized full-length PERV, five of them being replication-competent and four of them being chromosomally as...

journal_title：Virology

authors： Niebert M,Tönjes RR

更新日期：2003-09-01 00:00:00

abstract：:Puumala (PUUV) and Hantaan (HTNV) viruses are hantaviruses within the family Bunyaviridae and associated with Hemorrhagic Fever with Renal Syndrome (HFRS) in humans. Little is known about how these viruses interact with host cells, though pathogenic hantaviruses interact with α(v)β(3) integrin. To study host cell inte...

journal_title：Virology

authors： Higa MM,Petersen J,Hooper J,Doms RW

更新日期：2012-02-20 00:00:00

abstract：:Human papillomavirus type 16 (HPV-16) is the most common papillomavirus genome found in human cervical cancers and its DNA is capable of immortalizing primary keratinocytes in vitro. When expressed by their native promoter, two separate HPV-16 oncogenes, E6 and E7, cooperate to immortalize primary human keratinocytes....

journal_title：Virology

authors： Stöppler MC,Straight SW,Tsao G,Schlegel R,McCance DJ

更新日期：1996-09-01 00:00:00

abstract：:Transcriptionally targeted MLV-based ProCon vectors allow expression of the transduced gene in a promoter-specific manner by replacement of the viral U3 region with a heterologous promoter. In order to evaluate the effects of sequence elements present in ProCon vectors on transgene expression (enhanced green fluoresce...

journal_title：Virology

authors： Hlavaty J,Portsmouth D,Stracke A,Salmons B,Günzburg WH,Renner M

更新日期：2004-12-05 00:00:00

abstract：:The proteins encoded by open reading frame 3 (ORF3) of the umbraviruses pea enation mosaic virus-2 and tobacco mottle virus, like that of groundnut rosette virus, mediated the movement of viral RNA through the phloem of infected Nicotiana benthamiana or N. clevelandii plants when they were expressed from chimeric toba...

journal_title：Virology

authors： Ryabov EV,Robinson DJ,Taliansky M

更新日期：2001-09-30 00:00:00

abstract：:Lysolecithin permeabilization of vaccinia virus-infected cells was employed to prepare extracts that support faithful transcription initiation in vitro on plasmids possessing early, intermediate, and late viral gene promoters. Conditions which optimize transcription from each promoter were defined. The in vitro system...

journal_title：Virology

authors： Condit RC,Lewis JI,Quinn M,Christen LM,Niles EG

更新日期：1996-04-01 00:00:00

abstract：:The physical stability of virions of Cucumber mosaic virus (CMV) mutants was investigated to determine if relative stability correlated with efficiency of aphid transmission. Virion stability was evaluated by a urea disruption assay and by testing the infectivity of virus following purifications. All viruses were infe...

journal_title：Virology

authors： Ng JC,Josefsson C,Clark AJ,Franz AW,Perry KL

更新日期：2005-02-05 00:00:00

abstract：:We determined the complete nucleotide sequence of the New World simian foamy virus (FV) from spider monkey (SFVspm). Starting from a conserved region in the integrase (IN) domain of the pol gene we cloned fragments of the genome up to the 5' end of the long terminal repeat (LTR) into plasmid vectors and elucidated the...

journal_title：Virology

authors： Thümer L,Rethwilm A,Holmes EC,Bodem J

更新日期：2007-12-05 00:00:00

abstract：:The rate of synthesis in vivo and the steady-state level of mRNA of four "model" herpes simplex virus type 1 (HSV-1) genes were measured as a function of high levels of alpha-gene products. The genes studied were ICP4 (alpha), deoxy-UTPase (beta), VP5 (beta gamma), and glycoprotein C (gC, gamma). Accumulation of high ...

journal_title：Virology

authors： Zhang YF,Devi-Rao GB,Rice M,Sandri-Goldin RM,Wagner EK

更新日期：1987-03-01 00:00:00

abstract：:To identify ranavirus virulence genes, we engineered Frog Virus 3 (FV3) knockout (KO) mutants defective for a putative viral caspase activation and recruitment domain-containing (CARD) protein (Δ64R-FV3) and a β-hydroxysteroid dehydrogenase homolog (Δ52L-FV3). Compared to wild type (WT) FV3, infection of Xenopus tadpo...

journal_title：Virology

authors： Andino Fde J,Grayfer L,Chen G,Chinchar VG,Edholm ES,Robert J

更新日期：2015-11-01 00:00:00

abstract：:The location of nucleotide sequences important in determining the extent of cowpea mosaic virus M-RNA accumulation in cowpea protoplasts has been analyzed by deletion mutagenesis of full-length cDNA clones from which infectious transcripts can be produced in vitro. The results suggest that cis-acting sequences which d...

journal_title：Virology

authors： Rohll JB,Holness CL,Lomonossoff GP,Maule AJ

更新日期：1993-04-01 00:00:00

abstract：:HIV-1 transcytosis has been proposed as a potential mechanism allowing the virus to cross the epithelium during mucosal transmission. Epitopes of the HIV-1 envelope involved in this process have not been identified yet. Here, we assessed a large panel of HIV neutralizing antibodies recognizing well-characterized epito...

journal_title：Virology

authors： Chomont N,Hocini H,Gody JC,Bouhlal H,Becquart P,Krief-Bouillet C,Kazatchkine M,Bélec L

更新日期：2008-01-20 00:00:00

abstract：:Tetherin (ST2/CD317) is a cellular protein that restricts the release from cells of some enveloped viruses including HIV-1. To examine if influenza virus is affected by tetherin, MDCK cells constitutively expressing human tetherin and control MDCK cells were infected with influenza virus. No difference was observed in...

journal_title：Virology

authors： Watanabe R,Leser GP,Lamb RA

更新日期：2011-08-15 00:00:00

abstract：:Turnip crinkle carmovirus (TCV) has a uniquely high recombination frequency and nonrandom cross-over site distribution among the recombining TCV-associated satellite RNAs. An in vitro system has been developed that includes a partially purified TCV replicase preparation (RdRp) and chimeric RNAs that resemble the putat...

journal_title：Virology

authors： Nagy PD,Simon AE

更新日期：1998-09-30 00:00:00

abstract：:The enteric adenovirus type 40 strain Dugan (Ad40) cannot be passaged in HeLa cells, but will grow in 293 cells, which express Ad5 E1 functions. To determine the reason for this limited host range, KB cell lines expressing Ad2 E1A, E1B, or E1A + E1B (L. E. Babiss, C. S. H. Young, P. B. Fisher, and H. S. Ginsberg, 1983...

journal_title：Virology

authors： Mautner V,Mackay N,Steinthorsdottir V

更新日期：1989-08-01 00:00:00

abstract：:The tobamoviruses, tobacco mosaic virus (TMV) and Odontoglossum ringspot virus (ORSV), differ in the range of plant species that each can systemically infect. Both viruses systemically infect Nicotiana benthamiana similarly, but differ in the ability to systemically infect tobacco (N. tabacum). ORSV is confined to the...

journal_title：Virology

authors： Hilf ME,Dawson WO

更新日期：1993-03-01 00:00:00

abstract：:Human adenovirus type 3 (HAdV-B3) has an apparently stable genome yet remains a major circulating and problematic respiratory pathogen. Comparisons of the prototype genome to genomes from three current field strains, including two isolated from epidemics, and a laboratory strain, yielded small-scale nucleotide variati...

journal_title：Virology

authors： Mahadevan P,Seto J,Tibbetts C,Seto D

更新日期：2010-02-05 00:00:00

abstract：:Re-emergence of ZIKV has caused infections in more than 1.5 million people. The molecular mechanism and pathogenesis of ZIKV is not well explored due to unavailability of adequate model and lack of publically accessible resources to provide information of ZIKV-Human protein interactome map till today. This study made ...

journal_title：Virology

authors： Gurumayum S,Brahma R,Naorem LD,Muthaiyan M,Gopal J,Venkatesan A

更新日期：2018-01-15 00:00:00

abstract：:A nonhemagglutinating mutant of a 1978 isolate of canine parvovirus (CPV) was derived after repeated passages in the NLFK feline kidney cell line. The mutant CPV was antigenically indistinguishable from wild-type virus when tested with 82 monoclonal antibodies, and it replicated in cat and dog cell lines in culture. S...

journal_title：Virology

authors： Parrish CR,Burtonboy G,Carmichael LE

更新日期：1988-03-01 00:00:00

abstract：:SIV/Mne circle junctions were amplified by the polymerase chain reaction (PCR) and cloned in a bacterial plasmid. Sequence analysis of clones isolated from 11 independent PCRs reveals that the start site for plus DNA synthesis is 5' ACTG. . ., and thus an asymmetric cleavage must occur during viral integration. In add...

journal_title：Virology

authors： Randolph CA,Champoux JJ

更新日期：1993-06-01 00:00:00

abstract：:We have examined the synthesis of the T protein of the human polyomavirus, JCV, during productive infection in primary cultures of human fetal glial and kidney cells. Immunoprecipitation of protein extracts from virus infected cells revealed that the JCV large T protein from both the prototype Mad and HEK adapted stra...

journal_title：Virology

authors： Major EO,Traub RG

更新日期：1986-01-15 00:00:00

abstract：:Two small viral proteins (DGBp1 and DGBp2) have been proposed to act in a concerted manner to aid intra- and intercellular trafficking of carmoviruses though the distribution of functions and mode of action of each protein partner are not yet clear. Here we have confirmed the requirement of the DGBps of Pelargonium fl...

journal_title：Virology

authors： Martínez-Turiño S,Hernández C

更新日期：2011-05-10 00:00:00

abstract：:Methods currently used for HIV-1 viral load measurements are very sensitive, but cannot distinguish between infectious and noninfectious particles. Here we describe the development of a novel, sensitive, and highly reproducible method that allows rapid isolation and quantification of infectious particles from patient ...

journal_title：Virology

authors： Rusert P,Fischer M,Joos B,Leemann C,Kuster H,Flepp M,Bonhoeffer S,Günthard HF,Trkola A

更新日期：2004-08-15 00:00:00