Abstract:
:SIV/Mne circle junctions were amplified by the polymerase chain reaction (PCR) and cloned in a bacterial plasmid. Sequence analysis of clones isolated from 11 independent PCRs reveals that the start site for plus DNA synthesis is 5' ACTG. . ., and thus an asymmetric cleavage must occur during viral integration. In addition, most of the sequences found resulted from the ligation of aberrant proviral DNA ends that were apparently generated by priming errors, primer removal errors, or integrase processing errors. The results suggest that in this virus, as in Moloney murine leukemia virus, two good ends may be required for efficient integration.
journal_name
Virologyjournal_title
Virologyauthors
Randolph CA,Champoux JJdoi
10.1006/viro.1993.1329subject
Has Abstractpub_date
1993-06-01 00:00:00pages
851-4issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(83)71329-2journal_volume
194pub_type
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