Influenza virus is not restricted by tetherin whereas influenza VLP production is restricted by tetherin.

Abstract:

:Tetherin (ST2/CD317) is a cellular protein that restricts the release from cells of some enveloped viruses including HIV-1. To examine if influenza virus is affected by tetherin, MDCK cells constitutively expressing human tetherin and control MDCK cells were infected with influenza virus. No difference was observed in infectious titers, at 24 h or 48 h post-infection. In contrast, tetherin expression inhibited influenza virus-like particle (VLP) release into the media. Expression of the HIV protein Vpu overcame the tetherin block of influenza virus VLPs. A human tetherin mutant that lacks a C-terminal GPI anchor attachment signal (tetherin-ΔGPI) was constructed to test if this mutant could be incorporated into the released virus or VLP particles. Whereas tetherin-ΔGPI was incorporated into influenza VLPs it was not incorporated into influenza virions. Taken together these data suggest that influenza virions may contain a tetherin antagonist.

journal_name

Virology

journal_title

Virology

authors

Watanabe R,Leser GP,Lamb RA

doi

10.1016/j.virol.2011.05.006

subject

Has Abstract

pub_date

2011-08-15 00:00:00

pages

50-6

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(11)00219-4

journal_volume

417

pub_type

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