Abstract:
:Human papillomavirus type 16 (HPV 16) is often found in cervical carcinomas, while HPV 6 is frequently associated with benign genital lesions. We have compared the abilities of the E7 transforming proteins of HPV 6 and 16 to transform various established and primary rodent cells by using the same heterologous promoter system. HPV 16 E7 efficiently induced anchorage-independent growth of all the rodent cell lines tested and immortalized or cooperated with ras in transforming primary rat cells. On the other hand, the transforming activity of HPV 6 E7 was lower and was restricted. By construction of chimeras of HPV 6 and 16 E7, we found that the difference in transforming activity between the two E7 proteins was mainly determined by the difference in their 30 N-terminal amino acid residues, although some activities seem to be slightly affected by differences in their residual C-terminal portions.
journal_name
Virologyjournal_title
Virologyauthors
Takami Y,Sasagawa T,Sudiro TM,Yutsudo M,Hakura Adoi
10.1016/0042-6822(92)90014-gsubject
Has Abstractpub_date
1992-02-01 00:00:00pages
489-95issue
2eissn
0042-6822issn
1096-0341journal_volume
186pub_type
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