Characterization of the leader papain-like proteinase of MHV-A59: identification of a new in vitro cleavage site.

Abstract:

:Sequence analysis of the mouse hepatitis virus, strain A59 (MHV-A59) genome predicts the presence of two papain-like proteinases encoded within the first open reading frame (ORF 1a) of the replicase gene. The more 5' of these domains, the leader papain-like proteinase, is responsible for the cleavage of the amino terminal protein, p28. The core of this proteinase domain was defined to between amino acids 1084 and 1316 from the beginning of ORF 1a. Through the use of deletion analysis coupled with in vitro expression, we studied the role of the coding region between p28 and the leader papain-like proteinase on the cleavage of p28 itself. Expression of a series of deletion mutants showed processing of p28, albeit at lower levels. Reduced p28 production resulting from a 0.4-kb deletion positioned between p28 and the proteinase domain suggests an involvement of this region in catalytic processing. Some mutants displayed cleavage patterns indicative of a second cleavage site. Interestingly, this new cleavage site identified in vitro maps to a position similar to the expected cleavage site of a p65 polypeptide detected in MHV-A59-infected cells. Mutagenesis of the catalytic His1272 residue demonstrates that both cleavages observed are mediated by the leader papain-like proteinase encoded in ORF 1a.

journal_name

Virology

journal_title

Virology

authors

Bonilla PJ,Hughes SA,Piñón JD,Weiss SR

doi

10.1006/viro.1995.1281

subject

Has Abstract

pub_date

1995-06-01 00:00:00

pages

489-97

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(85)71281-0

journal_volume

209

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Protein composition of the vaccinia virus mature virion.

    abstract::The protein content of vaccinia virus mature virions, purified by rate zonal and isopycnic centrifugations and solubilized by SDS or a solution of urea and thiourea, was determined by the accurate mass and time tag technology which uses both tandem mass spectrometry and Fourier transform-ion cyclotron resonance mass s...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2006.08.025

    authors: Resch W,Hixson KK,Moore RJ,Lipton MS,Moss B

    更新日期:2007-02-05 00:00:00

  • Determination of the position of the boundaries of the terminal repetitive sequences within the genome of molluscum contagiosum virus type 1 by DNA nucleotide sequence analysis.

    abstract::The repetitive DNA sequences of the genome of Molluscum contagiosum virus type 1 (MCV-1) have been localized within the terminal regions of the viral genome corresponding to the BamHI MCV-1 DNA fragments B (18 kbp; 0 to 0.095 map units (m.u.)) and E (10.5 kbp; 0.944 to 1 m.u.). The fine mapping of these particular reg...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1993.1050

    authors: Bugert JJ,Raab K,Rösen-Wolff A,Janssen W,Darai G

    更新日期:1993-01-01 00:00:00

  • Delineation of a 16 amino acid sequence that forms a core DNA recognition motif in the Epstein-Barr virus EBNA-1 protein.

    abstract::EBNA-1 is the sole virally specified protein required for replication of the Epstein-Barr virus latency origin and also modulates its own expression. Both of these functions are mediated through specific DNA binding. We created a series of amino acid substitutions across the previously identified DNA-binding domain of...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1669

    authors: Chen MR,Zong J,Hayward SD

    更新日期:1994-12-01 00:00:00

  • Nucleotide sequences within the U5 region of the viral RNA genome are the major determinants for an human immunodeficiency virus type 1 to maintain a primer binding site complementary to tRNA(His).

    abstract::The initiation of reverse transcription of the human immunodeficiency virus type 1 (HIV-1) genome requires cellular tRNA(Lys,3) as a primer and occurs at a site in the viral RNA genome, designated as the primer binding site (PBS), which is complementary to the 3'-terminal 18 nucleotides of tRNA(Lys,3). We previously d...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0658

    authors: Zhang Z,Kang SM,LeBlanc A,Hajduk SL,Morrow CD

    更新日期:1996-12-15 00:00:00

  • Lipid selection in the assembly of the phospholipid bilayer membrane of the lipid-containing bacteriophage PR4.

    abstract::Phage PR4 contains a lipid bilayer within the phage protein capsid. The phospholipids of the bilayer are derived from those of the host. We report that phage morphogenesis selects against the unusually bulky phospholipids synthesized by Escherichia coli grown in the presence of various sugar alcohols. These data indic...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1004

    authors: Myung H,Cronan JE Jr

    更新日期:1994-01-01 00:00:00

  • The HBV-producing cell line HepG2-4A5: a new in vitro system for studying the regulation of HBV replication and for screening anti-hepatitis B virus drugs.

    abstract::All tissue culture systems for propagating HBV employed so far make use of tandemly arranged HBV genomes usually under the control of strong foreign promoters. Thus these systems are helpful for virus production but are of limited value in the investigation of the regulation of HBV replication or of the extent to whic...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0049

    authors: Weiss L,Kekulè AS,Jakubowski U,Bürgelt E,Hofschneider PH

    更新日期:1996-02-01 00:00:00

  • The nucleotide sequence of the polyhedrin gene region from the multicapsid baculovirus of Orgyia pseudotsugata.

    abstract::The nucleotide sequence was determined of a 1.6-kb restriction fragment containing the polyhedrin gene of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata (OpMNPV). In addition, both the 5' and 3' termini of the polyhedrin mRNA were located in the flanking sequences using S1 mapping procedures. 5' Le...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(86)90031-0

    authors: Leisy D,Rohrmann G,Beaudreau G

    更新日期:1986-09-01 00:00:00

  • Loss of the N-linked glycosylation site at position 386 in the HIV envelope V4 region enhances macrophage tropism and is associated with dementia.

    abstract::HIV infects macrophages and microglia in the central nervous system (CNS). Mechanisms that enhance HIV macrophage/microglial tropism are not well understood. Here, we identify an HIV Env variant in the V4 region of gp120, Asp 386 (D386), that eliminates an N-linked glycosylation site at position 386, enhances viral re...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2007.05.029

    authors: Dunfee RL,Thomas ER,Wang J,Kunstman K,Wolinsky SM,Gabuzda D

    更新日期:2007-10-10 00:00:00

  • Begomovirus genetic diversity in the native plant reservoir Solanum nigrum: Evidence for the presence of a new virus species of recombinant nature.

    abstract::We examined the native plant host Solanum nigrum as reservoir of genetic diversity of begomoviruses that cause the tomato yellow leaf curl disease (TYLCD) emerging in southern Spain. Presence of isolates of all the species and strains found associated with TYLCD in this area was demonstrated. Mixed infections were com...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2006.02.028

    authors: García-Andrés S,Monci F,Navas-Castillo J,Moriones E

    更新日期:2006-07-05 00:00:00

  • Hexagonal organization of Moloney murine leukemia virus capsid proteins.

    abstract::To help elucidate the mechanisms by which retrovirus structural proteins associate to form virus particles, we have examined membrane-bound assemblies of Moloney murine leukemia virus (M-MuLV) capsid (CA) proteins. Electron microscopy and image reconstruction techniques showed that CA dimers appear to function as orga...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.2002.1452

    authors: Mayo K,McDermott J,Barklis E

    更新日期:2002-06-20 00:00:00

  • Protein S and Gas6 induce efferocytosis of HIV-1-infected cells.

    abstract::Efferocytosis, the phagocytic clearance of apoptotic cells, can provide host protection against certain types of viruses by mediating phagocytic clearance of infected cells undergoing apoptosis. It is known that HIV-1 induces apoptosis and HIV-1-infected cells are efferocytosed by macrophages, although its molecular m...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2017.12.025

    authors: Chua BA,Ngo JA,Situ K,Ramirez CM,Nakano H,Morizono K

    更新日期:2018-02-01 00:00:00

  • JNK phosphorylation, induced during dengue virus infection, is important for viral infection and requires the presence of cholesterol.

    abstract::Infection with a broad diversity of viruses often activates host cell signaling pathways including the mitogen-activated protein kinase pathway. The present study established that dengue virus infection of human macrophages activates Jun NH(2)-terminal kinase (JNK) and the p38 MAPKs pathways. The activation was observ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2009.10.019

    authors: Ceballos-Olvera I,Chávez-Salinas S,Medina F,Ludert JE,del Angel RM

    更新日期:2010-01-05 00:00:00

  • Virus like particle based strategy to elicit HIV-protective antibodies to the alpha-helic regions of gp41.

    abstract::Natural antibodies to gp41 inhibit HIV-1 replication through the recognition of two different regions, corresponding to the leucine zipper motif in the HR1 alpha-helix and to another motif within HR2 region, hosting 2F5 and 4E10 epitope. This study aimed at reproducing such protective responses through VLP vaccination...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2012.05.005

    authors: Pastori C,Tudor D,Diomede L,Drillet AS,Jegerlehner A,Röhn TA,Bomsel M,Lopalco L

    更新日期:2012-09-15 00:00:00

  • VPg unlinkase/TDP2 in cardiovirus infected cells: Re-localization and proteolytic cleavage.

    abstract::Cardioviruses cause diseases in many animals including, in rare cases, humans. Although they share common features with all picornaviruses, cardioviruses have unique properties that distinguish them from other family members, including enteroviruses. One feature shared by all picornaviruses is the covalent attachment ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2018.01.010

    authors: Maciejewski S,Ullmer W,Semler BL

    更新日期:2018-03-01 00:00:00

  • PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.

    abstract::Recently, we reported that PrP(Sc), a surrogate marker for prion disease, is associated with the cellular fraction of blood from scrapie-infected sheep using a ligand-based immunoassay. In the study reported here, we found that a subset of peripheral blood mononuclear cells is most likely to sequester PrP(Sc) during b...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2010.05.023

    authors: Edwards JC,Moore SJ,Hawthorn JA,Neale MH,Terry LA

    更新日期:2010-09-15 00:00:00

  • The release of bluetongue virus from infected cells and their superinfection by progeny virus.

    abstract::Immunoelectron microscopy using an anti-VP2 monoclonal antibody complexed to colloidal gold has been used to study the mechanism of bluetongue virus (BTV) release from infected cells. Examination of the BTV-infected cell surface revealed that viruses are released both as enveloped particles, by budding through the pla...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(89)90218-3

    authors: Hyatt AD,Eaton BT,Brookes SM

    更新日期:1989-11-01 00:00:00

  • Two adenovirus proteins with redundant activities in virus growth facilitates tripartite leader mRNA accumulation.

    abstract::Most adenovirus-specific mRNAs expressed late after infection originate from a single transcription unit, the so-called major late transcription unit. All mRNAs expressed from this unit have in common a 201 nucleotide-long spliced tripartite leader segment attached to their 5' ends. Human adenovirus mutants that carry...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1993.1234

    authors: Ohman K,Nordqvist K,Akusjärvi G

    更新日期:1993-05-01 00:00:00

  • Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo.

    abstract::Felis catus papillomavirus type 2 (FcaPV2) DNA is found in feline cutaneous squamous cell carcinomas (SCCs); however, its biological properties are still uncharacterized. In this study, we successfully expressed FcaPV2 E6 and E7 putative oncogenes in feline epithelial cells and demonstrated that FcaPV2 E6 binds to p53...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2016.05.017

    authors: Altamura G,Corteggio A,Pacini L,Conte A,Pierantoni GM,Tommasino M,Accardi R,Borzacchiello G

    更新日期:2016-09-01 00:00:00

  • Simvastatin and oseltamivir combination therapy does not improve the effectiveness of oseltamivir alone following highly pathogenic avian H5N1 influenza virus infection in mice.

    abstract::Nonspecific anti-inflammatory drugs have been purported to reduce the burden of severe influenza disease. We demonstrate that, unlike oseltamivir administration, simvastatin administration did not reduce morbidity, mortality, or viral load of mice infected with H1N1 or H5N1 viruses. No added benefit to the efficacy of...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2013.01.017

    authors: Belser JA,Szretter KJ,Katz JM,Tumpey TM

    更新日期:2013-04-25 00:00:00

  • Characterization of the integration site of the CMV mtr in a tumor cell line.

    abstract::Previous studies have shown that a 558-bp fragment of human cytomegalovirus (CMV) DNA contained within pCM4127 and designated CMV mtr can morphologically transform rodents cells in vitro. By cotransformation with pCM4127 and a plasmid conferring G418 resistance, pSV2neo, morphologically transformed NIH3T3 cell lines w...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(87)90437-5

    authors: Buonaguro FM,McDougall JK,Galloway DA

    更新日期:1987-01-01 00:00:00

  • Assessment and optimization of rolling circle amplification protocols for the detection and characterization of badnaviruses.

    abstract::The genus Badnavirus is characterized by members that are genetically and serologically heterogeneous which presents challenges for their detection and characterization. The presence of integrated badnavirus-like sequences in some host species further complicates detection using PCR-based protocols. To address these c...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2019.01.013

    authors: Sukal AC,Kidanemariam DB,Dale JL,Harding RM,James AP

    更新日期:2019-03-01 00:00:00

  • Loss of HIV-specific memory B-cells as a potential mechanism for the dysfunction of the humoral immune response against HIV.

    abstract::A central, yet unresolved issue in the pathogenesis of HIV disease is the mechanism of antibody perturbation. In this study, HIV-specific memory B-cells were quantified in groups of infected subjects and compared with memory responses to other antigens and antibody titers. HIV-specific memory B-cell responses were vig...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2009.11.003

    authors: Bussmann BM,Reiche S,Bieniek B,Krznaric I,Ackermann F,Jassoy C

    更新日期:2010-02-05 00:00:00

  • Select resistance-associated mutations in blood are associated with lower CSF viral loads and better neuropsychological performance.

    abstract:BACKGROUND:When antiretroviral therapy does not fully suppress HIV replication, suboptimal levels of antiretrovirals can select for antiretroviral resistant variants of HIV. These variants may exhibit reduced replication capacity and result in lower viral loads in blood. Our study evaluated whether antiretroviral resis...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2009.08.007

    authors: Hightower GK,Letendre SL,Cherner M,Gibson SA,Ellis RJ,Wolfson TJ,Gamst AC,Ignacio CC,Heaton RK,Grant I,Richman DD,Smith DM,HNRC Group.

    更新日期:2009-11-25 00:00:00

  • Structure of the M2 protein gene of sonchus yellow net virus.

    abstract::The nucleotide sequence of the gene immediately following the nucleocapsid protein gene of sonchus yellow net virus (SYNV), a plant rhabdovirus, is presented. Serological reactions of SYNV proteins with antibodies elicited by a fusion protein constructed from the sequenced gene indicate that this gene encodes an SYNV ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(87)90190-5

    authors: Heaton LA,Zuidema D,Jackson AO

    更新日期:1987-11-01 00:00:00

  • Increase in the relative fitness of a plant virus RNA associated with its recombinant nature.

    abstract::A recombination event at the 3' end of RNA 3 in a pseudorecombinant virus (C1C2T3) having RNAs 1 and 2 from TrK7-cucumber mosaic virus (CMV) and RNA 3 from P-tomato aspermy virus (TAV) was detected by ribonuclease protection assay (RPA). Sequence analysis of the 3' end of RNA 3 of C1C2T3 and of its parental CMV and TA...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1496

    authors: Fernández-Cuartero B,Burgyán J,Aranda MA,Salánki K,Moriones E,García-Arenal F

    更新日期:1994-09-01 00:00:00

  • Translational studies with turnip yellow mosaic virus RNAs isolated from major and minor virus particles.

    abstract::The major and minor nucleoprotein components in turnip yellow mosaic virus (TYMV) preparations have been fractionated by four cycles of CsCl gradient equilibrium centrifugations. RNAs isolated from the various fractions were translated in a rabbit reticulocyte cell-free system. Three classes of RNAs were studied: geno...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(79)90170-3

    authors: Mellema JR,Benicourt C,Haenni AL,Noort A,Pleij CW,Bosch L

    更新日期:1979-07-15 00:00:00

  • Impact of macrophage and dendritic cell subset elimination on antiviral immunity, viral clearance and production of type 1 interferon.

    abstract::We report herein that vesicular stomatitis virus (VSV) induced a concurrent primary Th1 (T helper 1) and Th2 cytokine response detectable ex vivo. Liposome-encapsulated clodronate-mediated elimination of CD8- marginal dendritic cells (DCs) and splenic macrophages (m Phi), but not CD8+ interdigitating DCs, prior to inf...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2005.07.031

    authors: Ciavarra RP,Taylor L,Greene AR,Yousefieh N,Horeth D,van Rooijen N,Steel C,Gregory B,Birkenbach M,Sekellick M

    更新日期:2005-11-25 00:00:00

  • Palmitoylation of the cysteine-rich endodomain of the SARS-coronavirus spike glycoprotein is important for spike-mediated cell fusion.

    abstract::The SARS-coronavirus (SARS-CoV) is the etiological agent of the severe acute respiratory syndrome (SARS). The SARS-CoV spike (S) glycoprotein mediates membrane fusion events during virus entry and virus-induced cell-to-cell fusion. The cytoplasmic portion of the S glycoprotein contains four cysteine-rich amino acid cl...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2006.10.034

    authors: Petit CM,Chouljenko VN,Iyer A,Colgrove R,Farzan M,Knipe DM,Kousoulas KG

    更新日期:2007-04-10 00:00:00

  • Enhancement of dengue virus translation: role of the 3' untranslated region and the terminal 3' stem-loop domain.

    abstract::An essential step for a productive infection by the dengue flavivirus (DEN) is translation of the m(7)G-capped, nonpolyadenylated positive-sense RNA genome. We have recently identified sequences within the DEN 3' untranslated region (UTR) that modulate viral translation. Here, we show that the DEN type 2 (DEN2) 3'UTR ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2004.08.004

    authors: Holden KL,Harris E

    更新日期:2004-11-10 00:00:00

  • Activities and substrate specificity of the evolutionarily conserved central domain of retroviral integrase.

    abstract::The retroviral integrase (IN) is a virus-encoded enzyme that is essential for insertion of viral DNA into the host chromosome. In order to map and define the properties of a minimal functional domain for this unique viral enzyme, a series of N- and C-terminal deletions of both Rous sarcoma virus (RSV) and human immuno...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/s0042-6822(95)80060-3

    authors: Kulkosky J,Katz RA,Merkel G,Skalka AM

    更新日期:1995-01-10 00:00:00