Abstract:
:Mouse mammary tumour virus (MMTV) encodes a viral superantigen (Sag) and a negative acting factor (Naf) which share parts of their coding sequence. Using 2-dimensional gel electrophoresis (2D-DIGE), we could show that at least 10 different cellular proteins were differentially expressed in Naf positive cells. Also, luciferase reporter expression was down-regulated in Naf expressing cells independent of the promoter used and further experiments suggested that this effect was due in part to a decrease in cellular growth rates. Although in Naf positive cells expression of the major sag containing transcript was strongly induced by the synthetic glucocorticoid dexamethasone, the hormone analogue neither influenced luciferase expression nor mRNA expression of selected cellular proteins identified by 2D-DIGE. Taken together, these data support the previous finding that Naf and Sag have separable activities and suggest that Naf may play a role in modulating host cell gene expression during MMTV infection.
journal_name
Virologyjournal_title
Virologyauthors
Metzner C,Salmons B,Gunzburg WH,Gemeiner M,Miller I,Gesslbauer B,Kungl A,Dangerfield JAdoi
10.1016/j.virol.2005.10.029subject
Has Abstractpub_date
2006-03-01 00:00:00pages
139-50issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(05)00724-5journal_volume
346pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::This report shows that (S)CARNA 5, the satellite of cucumber mosaic virus (CMV) strain S, directs the synthesis of two small proteins in a wheat germ cell-free translation system. The two proteins are produced by the translation of both (S)CARNA 5 purified from CMV virions and the (+) strand purified from double-stran...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90147-9
更新日期:1986-07-30 00:00:00
abstract::Sequence analysis of the mouse hepatitis virus, strain A59 (MHV-A59) genome predicts the presence of two papain-like proteinases encoded within the first open reading frame (ORF 1a) of the replicase gene. The more 5' of these domains, the leader papain-like proteinase, is responsible for the cleavage of the amino term...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1281
更新日期:1995-06-01 00:00:00
abstract::The NIa protein of potyviruses provides VPg and proteolytic functions during virus replication. It has also been shown to confer host genotype-specific movement functions in plants. Specifically, NIa from tobacco etch virus (TEV)-Oxnard, but not from most other strains, confers the ability to move long distances in Ni...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0416
更新日期:2000-08-01 00:00:00
abstract::The nucleotide sequence of the leader and the gene encoding the nucleocapsid protein (NP) of mumps virus Miyahara strain have been determined. The leader sequence is 55 nucleotides in length and the NP gene is 1845 nucleotides in length, exclusive of poly(A). The NP gene codes for a protein of 549 amino acids, with a ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90466-5
更新日期:1990-07-01 00:00:00
abstract::Mice are used as models for western equine encephalitis virus (WEEV) infection, but high mortality is generally only seen with intracranial or intranasal challenge, while peripheral inoculation results in approximately 50% mortality and is not dose-dependent. Hamsters were therefore studied as a model for WEEV infecti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.031
更新日期:2007-04-10 00:00:00
abstract::Human cytomegalovirus (HCMV) infects the chorioamnion, but whether these infections cause fetal membrane dysfunction remains poorly understood. We sought to assess whether guinea pig cytomegalovirus (GPCMV) infects amnion-derived cells in vitro, compare the inflammatory response of amnion cells to GPCMV and HCMV, and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.06.005
更新日期:2020-09-01 00:00:00
abstract::We analyzed various mutants of tomato leaf curl virus-India to investigate the role of ORFs AV3, AV2, and coat protein (CP) in viral replication, movement, and symptom development. The results of these studies indicate that ORF AV3 does not encode a protein. Plants inoculated with infectious DNA which contained deleti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0546
更新日期:1996-10-15 00:00:00
abstract::The complete nucleotide sequence of the middle component RNA (M RNA) of the comovirus bean pod mottle virus (BPMV) has been determined. The sequence consists of 3662 nucleotides and contains a single long open reading frame sufficient to code for a protein of 113,353 Da. The proteolytic processing sites within this pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90155-5
更新日期:1991-07-01 00:00:00
abstract::Infection with a broad diversity of viruses often activates host cell signaling pathways including the mitogen-activated protein kinase pathway. The present study established that dengue virus infection of human macrophages activates Jun NH(2)-terminal kinase (JNK) and the p38 MAPKs pathways. The activation was observ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.10.019
更新日期:2010-01-05 00:00:00
abstract::Few human endogenous retroviruses (HERVs) have been extensively studied in non-human primates. Such investigations have demonstrated that several element classes are primate unique, contain members with important biological function, are conserved in specific primate lineages, and have in some cases expanded in copy n...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.045
更新日期:2005-04-10 00:00:00
abstract::Beet necrotic yellow vein virus (BNYVV) has a quadripartite plus-strand RNA genome in which the two smallest genome components, RNA 3 and 4, are not necessary for virus multiplication in leaves. Infectious transcripts of BNYVV RNA 3 and 4 have already been described (V. Ziegler-Graff, S. Bouzoubaa, I. Jupin, H. Guille...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90131-1
更新日期:1989-09-01 00:00:00
abstract::Toscana (TOS) virus stocks strongly interfering with standard virus replication were obtained by sequential passages of virus in suckling mouse brain. Characterization of viral RNAs in these stocks showed the presence of a heterogeneous population of defective RNA molecules derived from the L genomic segment, in both ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9195
更新日期:1998-06-20 00:00:00
abstract::The behavior of two genetically different molecular clones of visna virus KV1772-kv72/67 and LV1-1KS1 was compared in vivo and in vitro. On intracerebral inoculation, clone KV1772-kv72/67 induced a similar response in five sheep as has already been reported with neurovirulent derivates of visna virus. Virus was freque...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8428
更新日期:1997-03-17 00:00:00
abstract::Therapeutic immunization of HIV-1-infected individuals may induce and/or enhance HIV-1-specific immune responses and decrease the dependency on antiretroviral drug treatment. However, repeated immunizations with live-recombinant vectors may induce vector-specific immune responses that interfere with the elicitation of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1722
更新日期:2002-12-05 00:00:00
abstract::Nipah virus (NiV) causes severe encephalitis in humans, with high mortality. NiV nonstructural C protein (NiV-C) is essential for its pathogenicity, but its functions are unclear. In this study, we focused on NiV-C trafficking in cells and found that it localizes predominantly in the cytoplasm but partly in the nucleu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.07.013
更新日期:2016-10-01 00:00:00
abstract::Insertion of a functional splicing cassette into a construct containing the HIV-1 poly(A) site followed by the adenovirus L3 poly(A) site results in both specific stimulation of 3' end processing at the HIV-1 site and an increase in the steady-state levels of RNA processed at both sites. To further evaluate this influ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0556
更新日期:1996-10-15 00:00:00
abstract::We have previously adapted an experimental system which supports autonomous replication of human papillomavirus-11 (HPV-11) genomic DNA in a human squamous carcinoma cell line (SCC-4) following electroporation of linearized viral DNA. This system allows evaluation of HPV-11 transcription in the context of replicating ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0298
更新日期:1996-06-01 00:00:00
abstract::To better understand the evolution of the viral envelope glycoproteins (Env) in HIV-1 infected individuals who progress to AIDS maintaining an exclusive CCR5-using (R5) virus population, we cloned and sequenced the env gene of longitudinally obtained primary isolates. A shift in the electrostatic potential towards an ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.014
更新日期:2008-09-15 00:00:00
abstract::Seven envelope regions from two series of patient isolates have been molecularly cloned and analyzed for replication phenotypes and immunogenicity. Growth potential was analyzed for env sequences substituted into an HIV-1-NL4-3 backbone (NL4-3/env recombinants). Immunogenicity studies were conducted on secreted monome...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8445
更新日期:1997-03-03 00:00:00
abstract::We have used a microinjection approach to identify a domain of the simian virus 40 (SV40) structural proteins Vp2 and Vp3(Vp2/3) responsible for their nuclear transport. By using both synthetic peptides, containing small regions of Vp2/3 conjugated to bovine serum albumin (BSA), and beta-galactosidase-Vp3 fusion prote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90472-n
更新日期:1991-03-01 00:00:00
abstract::An immunodominant region of VP3, one of the two structural proteins of infectious bursal disease virus (IBDV strain 002-73), has been mapped by restriction site-specific deletion analysis and subcloning in Escherichia coli, followed by immunoblot analysis of the synthesized products. The epitope located within 58 amin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90457-m
更新日期:1991-10-01 00:00:00
abstract::The P150 and P90 replicase proteins of rubella virus (RUBV), a plus-strand RNA Togavirus, produce a unique cytoplasmic fiber network resembling microtubules. Pharmacological and mutagenic approaches were used to determine if these fibers functioned in virus replication. The pharmacological approach revealed that micro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.07.025
更新日期:2010-10-25 00:00:00
abstract::Mouse mammary tumor virus (MMTV) is a pH-dependent virus that uses mouse transferrin receptor 1 (TfR1) for entry into cells. Previous studies demonstrated that MMTV could induce pH 5-dependent fusion-from-with of mouse cells. Here we show that the MMTV envelope-mediated cell-cell fusion requires both the entry recepto...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.013
更新日期:2008-11-25 00:00:00
abstract::Hepatitis delta virus (HDV) encodes only one protein, the hepatitis delta antigen (HDAg). Two forms of HDAg, a large (27 kDa) and a small (24 kDa) one, participate in the various steps of HDV replication. To further understand the properties of HDAg, we have constructed recombinant baculoviruses and expressed both for...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)91227-l
更新日期:1992-09-01 00:00:00
abstract::In vivo infection with human immunodeficiency virus type 1 (HIV-1) leads to gradual depletion of CD4+ T lymphocytes from the peripheral blood and later from the lymphoid organs. The mechanism of CD4 cell depletion is not known. HIV can only replicate in dividing lymphocytes, but greater than 98% of the lymphocytes in ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8397
更新日期:1997-02-17 00:00:00
abstract::The V3 region of the HIV-1 envelope (Env) glycoprotein gp120 is a key functional domain yet it exhibits distinct mutational patterns across subtypes. Here an invariant residue (Ile 309) was replaced with Leu in 7 subtype C patient-derived Envs from recent infection and 4 related neutralizing antibody escape variants t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.04.010
更新日期:2010-08-15 00:00:00
abstract::Without a virus culture system, genetic analysis becomes the principal method to classify norovirus (NoV) strains. Currently, classification of NoV strains beneath the species level has been based on sequences from different regions of the viral genome. As a result, the phylogenetic insights of some virus were not app...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.015
更新日期:2006-03-15 00:00:00
abstract::Human papillomavirus type 16 (HPV 16) is often found in cervical carcinomas, while HPV 6 is frequently associated with benign genital lesions. We have compared the abilities of the E7 transforming proteins of HPV 6 and 16 to transform various established and primary rodent cells by using the same heterologous promoter...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90014-g
更新日期:1992-02-01 00:00:00
abstract::Adenovirus 12 (Ad12), but not adenovirus 2 or 5, induces metaphase chromosome fragility at four specific loci in humans: RNU1, RNU2, PSU1, and RN5S. As each of these sites corresponds to a tandemly repeated multigene family encoding a small, abundant structural RNA, we proposed that Ad12 hinders metaphase chromatin co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9512
更新日期:1999-02-01 00:00:00
abstract::Lipid binding properties which are dependent on exposure to acid pH are an important mechanism for the cellular entry pathway for some enveloped viruses and for other macromolecules. Cell-to-cell fusion can be used to demonstrate this function. La Crosse virus, a member of the family Bunyaviridae, fused BHK-21 cells f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90107-7
更新日期:1984-01-15 00:00:00