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In vivo and in vitro analyses of a Bombyx mori nucleopolyhedrovirus mutant lacking functional vfgf.

Abstract：

:All lepidopteran baculovirus genomes sequenced to date encode a viral fibroblast growth factor homolog (vfgf), suggesting that vfgf may play an important role in the infection cycle of lepidopteran baculoviruses. Here, we describe the characterization of a Bombyx mori nucleopolyhedrovirus (BmNPV) mutant lacking functional vfgf. We constructed a vfgf deletion mutant (BmFGFD) and characterized it in BmN cells and B. mori larvae. We observed that budded virus (BV) production was reduced in BmFGFD-infected BmN cells and B. mori larvae. The larval bioassays also revealed that deletion of vfgf did not reduce the infectivity; however, the mutant virus did take 20 h longer to kill B. mori larvae than wild-type BmNPV, when tested either by BV injection or by polyhedrin-inclusion body ingestion. These results suggest that BmNPV vfgf is involved in efficient virus production in BmN cells and B. mori larvae.

journal_name

Virology

journal_title

Virology

authors

Katsuma S,Horie S,Daimon T,Iwanaga M,Shimada T

doi

10.1016/j.virol.2006.07.008

subject

Has Abstract

pub_date

2006-11-10 00:00:00

pages

62-70

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(06)00447-8

journal_volume

355

pub_type

杂志文章

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