Abstract:
:Rice tungro bacilliform virus (RTBV) replication intermediates have been studied in rice plants infected with the virus. Unencapsidated virus-specific molecules were identified which had open circular, linear, supercoiled (SC), strong-stop, single-stranded, linear double-stranded hairpin, and double-stranded with single-stranded extension DNA forms. The structures of these different DNA forms were consistent with the replication model of cauliflower mosaic virus and support other results that reverse transcription is involved in the replication of RTBV. The existence of nonspecific and defective (+)-strand priming is suggested. The relative amount of SC DNAs differs in various tissues of the same plant and in the same tissue at different ages. This indicates host regulation of the virus replication cycle and a feedback regulatory mechanism in controlling the SC DNA level. There are no obvious differences in the composition of the replication intermediates between insect-infected and agroinoculated rice plants.
journal_name
Virologyjournal_title
Virologyauthors
Bao Y,Hull Rdoi
10.1006/viro.1994.1577subject
Has Abstractpub_date
1994-11-01 00:00:00pages
626-33issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(84)71577-7journal_volume
204pub_type
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