Abstract:
:The genome of the single-stranded DNA phage f1 contains an intergenic region (IG), 508-nucleotides long, that does not code for any known protein. By use of a system of chimeric plasmids haboring different f1 fragments, we had previously shown that this region contains, in addition to the f1 'functional origin' of DNA replication, a signal of less than 300 nucleotides required for efficient morphogenesis to occur ('morphogenetic signal'). In the present study, we have localized this signal to within a sequence of less that 60 nucleotides of almost perfect palindromic symmetry at the genet IV/IG border. We also present data indicating that the morphogenetic signal is not necessary for the synthesis of single-stranded DNA, but is necessary only at some later step during virion maturation.
journal_name
Virologyjournal_title
Virologyauthors
Dotto GP,Zinder NDdoi
10.1016/0042-6822(83)90136-8subject
Has Abstractpub_date
1983-10-15 00:00:00pages
252-6issue
1eissn
0042-6822issn
1096-0341journal_volume
130pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Clones have been constructed containing full-length cDNA copies of PEBV RNA1 and RNA2, flanked by the CaMV 35 S RNA promoter and the nopaline synthase terminator. The clones are infectious when inoculated onto Nicotiana benthamiana plants. Both the viral RNAs and the virus particles were identified in infected plants....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90488-b
更新日期:1992-04-01 00:00:00
abstract::The complete RNA sequence of Sabin 3 (LED3) used in vaccine in the United States has been determined. The LED3 Sabin 3 sequence contains the attenuating mutations at bases 472 and 2034 but differs from that published by Stanway et al. (Nucleic Acids Res., 11, 5629-5643, 1983) at two other base positions, 2493 and 6061...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90576-w
更新日期:1991-12-01 00:00:00
abstract::Because phosphorylation of the infectious bronchitis virus (IBV) nucleocapsid protein (N) may regulate its multiple roles in viral replication, the dynamics of N phosphorylation were examined. 32P-orthophosphate labeling and Western blot analyses confirmed that N was the only viral protein that was phosphorylated. Pul...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.04.029
更新日期:2005-08-15 00:00:00
abstract::Human papillomavirus type 16 (HPV-16) infections can in rare cases persist and cause lesions that may progress to cervical cancer. Cells in the lesions are not permissive for virus production, nor are cervical cancer cells. The intracellular environment is such that it prevents production of the highly immunogenic, vi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.002
更新日期:2007-12-20 00:00:00
abstract::Here we address the role of RIG-I and TLR3 in differential cytokine responses against Simian Virus 5 (SV5) and two distinct cytokine inducing SV5 mutants. IFN-beta and IL-6 secretion was induced by infection with P/V-CPI-, an SV5 mutant with P/V substitutions, and were reduced by either siRNA-mediated knockdown of RIG...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.014
更新日期:2010-02-05 00:00:00
abstract::Viruses that establish latent infection must maintain their DNA in the host nucleus through many cellular generations. Here we identify a novel mechanism by which the gammaherpesvirus Kaposi's sarcoma-associated herpesvirus (KSHV) may achieve this persistence in latently infected body cavity-based lymphoma (BCBL) cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9999
更新日期:1999-11-25 00:00:00
abstract::The requirement for the herpes simplex virus type 1 (HSV-1) protein Vmw65 (VP16) for activation of immediate early (IE) gene expression was examined in synchronized HeLa cells. Analyses of IE RNA levels were conducted during infection with a viral Vmw65 mutant, in1814. The results revealed an increased requirement for...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90695-l
更新日期:1992-07-01 00:00:00
abstract::The Fgf-3 protooncogene (previously called int-2) is a target of proviral insertion mutations in mammary tumors induced by the mouse mammary tumor virus (MMTV). These insertion mutations result in the transcriptional activation of Fgf-3, which is not normally expressed in the adult mammary gland. Previous mapping stud...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8794
更新日期:1997-11-10 00:00:00
abstract::Programmed Death-1 (PD-1), a member of T cell costimulatory molecules is expressed in high levels on antigen specific T cells during chronic viral infection, whereas PD-1 expression in the context of HIV-1 infected CD4+ T cells is not known. Here we report that productively infected CD4+ T cells lose PD-1, whereas bys...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.015
更新日期:2008-06-20 00:00:00
abstract::The P670 promoter of HPV16 directs transcription of the virus late genes in the differentiating epithelium. We found that CCAAT/enhancer binding protein beta (C/EBPbeta), a key transcription factor that induces the terminal differentiation of keratinocytes, enhanced the P670-driven transcription in transient reporter ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.025
更新日期:2006-03-01 00:00:00
abstract::The HIV-1 Nef protein causes a decrease in major histocompatibility complex (MHC) class I and CD4 molecule expression on the cell surface. To determine if Nef can affect components of the innate immune response, we assessed the ability of Nef to alter the cell surface expression of human CD1d. In cells co-expressing C...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.04.020
更新日期:2005-07-05 00:00:00
abstract::Many virus-encoded RGD-containing proteins have been reported to play important roles in virus attachment and entry. Here we report the identification and functional characterization of a gene encoding an RGD-containing protein (037L) from large yellow croaker iridovirus (LYCIV), a causative agent of epizootics among ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.07.004
更新日期:2006-11-25 00:00:00
abstract::Human Cytomegalovirus (HCMV) infection is compromised in cells lacking p53, a transcription factor that mediates cellular stress responses. In this study we have investigated compromised functional virion production in cells with p53 knocked out (p53KOs). Infectious center assays found most p53KOs released functional ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.07.021
更新日期:2016-10-01 00:00:00
abstract::HTLV-I infection is characterized by low viremia and restricted viral expression. While the mechanisms regulating viral latency are poorly understood, it is believed that interactions between viral and host cellular factor(s) are involved. Several lines of evidence indicate that HTLV-I provirus may be methylated in pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90649-v
更新日期:1991-05-01 00:00:00
abstract::Productive infection by human immunodeficiency virus type I (HIV-1) in the central nervous system (CNS) involves mainly macrophages and microglial cells. A frequency of less than 10% of human astrocytes is estimated to be infectable with HIV-1. Nonetheless, this relatively low percentage of infected astrocytes, but as...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.03.017
更新日期:2005-06-05 00:00:00
abstract::Recent reports indicated that vaccinia virus late mRNAs contain a unique 5' poly(A) leader sequence and that the in vitro translation of these mRNAs may be relatively cap-independent. These observations led us to examine the possibility that the mechanism of inhibition of host protein synthesis by vaccinia virus, like...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90556-5
更新日期:1992-06-01 00:00:00
abstract::Our previous studies have demonstrated that virus-specific cell-mediated cytotoxicity of sensitized leukocytes can be induced using clonal ginbuna crucian carp and their syngeneic cell lines. In the present study, we attempt to determine if virus-specific cytotoxic cell populations of fish express CD8alpha and TCRbeta...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.01.019
更新日期:2006-05-10 00:00:00
abstract::Neutralizing monoclonal antibodies 2F1 and 1C10, which are specific for VP7 of serotype 2 rotaviruses (G2), were used to select neutralization escape variants of the human serotype 2 rotavirus, DS-1. Neutralization survival patterns generated by monoclonal antibodies 2F1, 1C10, and RV5:3 indicated that 2F1 and 1C10 di...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1601
更新日期:1993-11-01 00:00:00
abstract::The genome of the sigma rhabdovirus of Drosophila melanogaster consists of six genes in the order 3' N-2-3-4-G-L 5'. The nucleotide sequences of the N and of the fourth genes were determined from cDNA clones. Each gene contained a single long open reading frame encoding polypeptides with predicted MW of 50 and 25 kDa,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1177
更新日期:1994-04-01 00:00:00
abstract::The expression of cytokines may influence the development of lymphoma in retrovirally infected animals in at least two ways: (1) cytokines in the tumor environment may stimulate the proliferation of tumor cells and/or (2) cytokines in the tumor environment may diminish the cell-mediated antitumor immune response. To e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9846
更新日期:1999-09-01 00:00:00
abstract::The requirement to replicate in both vertebrate and invertebrate hosts is thought to limit the introduction of genetic changes into the genome of arboviruses. Serial passage under laboratory conditions will overcome this limitation allowing for genetic changes to be introduced and affecting the virulence of the virus ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.03.030
更新日期:2011-06-20 00:00:00
abstract::Section of the sciatic nerve during the period of herpes simplex virus (HSV) latent infection was performed to evaluate residual latency in mouse dorsal root ganglion. In control mice without sciatic neurectomy, latency was present in 90-100%, while in those which underwent a neurectomy procedure, latent infection was...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90085-2
更新日期:1988-11-01 00:00:00
abstract::Human cytomegalovirus (HCMV) was recently shown to encode a large number of spliced mRNAs. While the nuclear export of unspliced viral transcripts has been extensively studied, the role of host mRNA export factors in HCMV mRNA trafficking remains poorly defined. We found that the eIF4AIII RNA helicase, a component of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.12.009
更新日期:2016-02-01 00:00:00
abstract::To gain new insights regarding the role of Ku, the DNA-PK DNA-binding component, during lentiviral DNA integration, we have investigated the HIV-1 replication in Ku80-depleted human cells. CEM4fx cells underexpressing the Ku80 factor were selected after transduction by a retroviral vector expressing the Ku80 full-leng...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1515
更新日期:2002-08-15 00:00:00
abstract::Recently, we reported that in vitro and in vivo persistent infection of neurons by lymphocytic choriomeningitis virus (LCMV) downregulated GAP43 expression, a protein involved in neuronal plasticity associated with learning and memory. Here, we investigated the transcriptional and posttranscriptional events involved. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8458
更新日期:1997-04-14 00:00:00
abstract::Strong evidence indicates that virions of mammalian reoviruses undergo proteolytic processing by acid-dependent cellular proteinases as an essential step in productive infection. Proteolytic processing takes the form of a series of cleavages of outer-capsid proteins final sigma3 and mu1/mu1C. Previous studies showed a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9434
更新日期:1998-11-25 00:00:00
abstract::Infectious bronchitis virus (IBV) produces six subgenomic (sg) mRNAs, each containing a 64 nucleotide (nt) leader sequence, derived from the 5' end of the genome by a discontinuous process. Several putative functional domains such as a papain-like proteinase (PL(pro)), main protease (M(pro)), RNA-dependent RNA polymer...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.03.032
更新日期:2004-06-20 00:00:00
abstract::The nucleotide and deduced amino acid sequence of the gene 4 of bovine rotavirus strain B223 is described. The open reading frame is predicted to encode a VP4 of 772 amino acids, shorter than described for any other rotavirus strain sequenced to date. B223 VP4 shows 70 to 73% similarity to other rotavirus VP4 proteins...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90191-q
更新日期:1992-11-01 00:00:00
abstract::Poliovirus replicons were constructed which contain the internal ribosome entry site (IRES) of encephalomyocarditis virus (EMCV) substituted for the poliovirus IRES. To monitor gene expression and encapsidation, the gene encoding firefly luciferase was substituted for the P1 gene. Replicons can be encapsidated followi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0433
更新日期:2000-08-01 00:00:00
abstract::A stem-loop structure, formed by a pair of inverted repeats during DNA replication, is a conserved feature at the origin of DNA replication among plant and animal viruses, bacteriophages and plasmids that replicate their genomes via the rolling-circle replication (RCR) mechanism. In this work, a head-to-tail tandem co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.017
更新日期:2007-06-20 00:00:00