An insertion mutation in the pol gene of Moloney murine leukemia virus results in temperature-sensitive pol maturation and viral replication.

abstract：:Analogous to transmission of human T-cell leukemia virus type 1 (HTLV-1) in vivo, an in vitro cell-to-cell infection model was established by coculturing MT-2 cells as virus donors and HUT78 cells as recipients. At a donor:recipient ratio of 1:2, cell fusion occurred and a new round of HTLV-1 genome replication was in...

journal_title：Virology

authors： Benovic S,Kok T,Stephenson A,McInnes J,Burrell C,Li P

更新日期：1998-05-10 00:00:00

abstract：:Cytotoxic T-cell clones raised against X-31 (H3N2) influenza virus in C57BL/6 mice can be directed against an influenza A virus subtype specific determinant (1). A representative T-cell clone (A3.1) has been used in combination with a set of genetically typed recombinant viruses, to show that the A/PR/8/34 nucleoprote...

journal_title：Virology

authors： Townsend AR,Skehel JJ,Taylor PM,Palese P

更新日期：1984-03-01 00:00:00

abstract：:Each copy of the inverted terminal repeat of vaccinia virus consists of 8 kb of DNA containing 9 ORFS flanked near the terminus of the genome by 4 kb of repetitive DNA which in turn contains blocks of tandem repeats. Using plasmids containing repetitive DNA as the external arm, we have generated deletions at both the ...

journal_title：Virology

authors： Perkus ME,Goebel SJ,Davis SW,Johnson GP,Norton EK,Paoletti E

更新日期：1991-01-01 00:00:00

abstract：:The mature flavivirus capsid protein (virion C) is commonly thought to be free in the cytoplasm of infected cells and to form a nucleocapsid-like complex with genomic RNA in mature virus particles. There is little sequence conservation among flavivirus virion C proteins, but they are similar in size (e.g., 99 amino ac...

journal_title：Virology

authors： Markoff L,Falgout B,Chang A

更新日期：1997-06-23 00:00:00

abstract：:We compared the outcome of different cellular and viral factors on the regulation of the HPV-16 early viral gene expression in trophoblastic and cervical cancer cells. A high variability of the long control (LCR) activity was observed, prompting us to evaluate the role of secreted factors in the control of the early g...

journal_title：Virology

authors： Weyn C,Vanderwinden JM,Rasschaert J,Englert Y,Fontaine V

更新日期：2011-03-30 00:00:00

abstract：:We examined the assembly processes of the capsid proteins of canine parvovirus (CPV) in mammalian and insect cells. In CPV-infected cells empty capsids assembled within 15 min, and then continued to form over the following 1 h, while full (DNA-containing) capsids were detected only after 60 min, and those accumulated ...

journal_title：Virology

authors： Yuan W,Parrish CR

更新日期：2001-01-20 00:00:00

abstract：:The yellow fever 17D virus is attenuated and used for human vaccination. Two of its substrains, 17D-204 and 17DD, are used for vaccine production. One of the remarkable properties of this vaccine is limited viral replication in the host but with significant dissemination of the viral mass, yielding a robust and long-l...

journal_title：Virology

authors： Marchevsky RS,Freire MS,Coutinho ES,Galler R

更新日期：2003-11-10 00:00:00

abstract：:The genes of the RF strain of bovine rotavirus have been cloned into pBR 322 following the synthesis and hybridization of cDNA transcribed from both strands of in vitro polyadenylated genomic RNA. Cloned rotavirus DNAs were assigned to most of the 11 genomic RNA segments by Northern blot hybridization. The complete se...

journal_title：Virology

authors： Cohen J,Lefevre F,Estes MK,Bremont M

更新日期：1984-10-15 00:00:00

abstract：:It was previously reported that integrins alpha2beta1, alpha4beta1, and alphaXbeta2 are involved in rotavirus cell infection. In this work we studied the role of integrin subunits alpha2, alpha4, and beta2 on the attachment of rotaviruses RRV and nar3 to MA104 cells. Integrin alpha2beta1 was found to serve as the bind...

journal_title：Virology

authors： Zárate S,Espinosa R,Romero P,Guerrero CA,Arias CF,López S

更新日期：2000-12-05 00:00:00

abstract：:To examine whether there are HBV variants not yet described which cannot express HBe due to a mutation in the pre-C region, and, if they exist, whether they cause a particular course of infection and disease, we analyzed the HBV genomes of a HBs/anti-HBe positive chronic carrier who had several episodes of acute reexa...

journal_title：Virology

authors： Raimondo G,Schneider R,Stemler M,Smedile V,Rodino G,Will H

更新日期：1990-11-01 00:00:00

abstract：:Hepatitis C virus (HCV) NS5A is a phosphoprotein that possesses a cryptic trans-activation activity. To investigate its potential role in viral replication, we searched for the cellular proteins interacting with NS5A protein by yeast two-hybrid screening of a human hepatocyte cDNA library. We identified a newly discov...

journal_title：Virology

authors： Tu H,Gao L,Shi ST,Taylor DR,Yang T,Mircheff AK,Wen Y,Gorbalenya AE,Hwang SB,Lai MM

更新日期：1999-10-10 00:00:00

abstract：:The influenza virus mRNAs are structurally similar to cellular mRNAs nevertheless; the virus promotes selective translation of viral mRNAs despite the inhibition of host cell protein synthesis. The infection proceeds normally upon functional impairment of eIF4E cap-binding protein, but requires functional eIF4A helica...

journal_title：Virology

authors： Yángüez E,Rodriguez P,Goodfellow I,Nieto A

更新日期：2012-01-20 00:00:00

abstract：:Ts1, a temperature-sensitive mutant of Moloney murine leukemia virus-TB (MoMuLV-TB), causes a progressive hindlimb paralytic disease in susceptible strains of mice. Previously, it has been shown that a single amino acid substitution, Val-25----Ile in gPr80env, is responsible for the temperature sensitivity, inefficien...

journal_title：Virology

authors： Kamps CA,Lin YC,Wong PK

更新日期：1991-10-01 00:00:00

abstract：:The complete nucleotide sequence of apple stem grooving virus (ASGV) genome has been determined. The genome is 6496 nucleotides in length excluding a 3'-terminal poly(A) tail and contains two overlapping open reading frames (ORFs). ORF1 begins at nucleotide position 37 and is terminated at position 6341, encoding a pr...

journal_title：Virology

authors： Yoshikawa N,Sasaki E,Kato M,Takahashi T

更新日期：1992-11-01 00:00:00

abstract：:Covalently closed circular dimers of phage lambda DNA have been found in Escherichia coli infected with lambda. These dimers can be formed by either the lambda Red or Int systems, by a nonrecombinational replicative mechanism requiring the activity of the lambda O and P genes or by joining of the cohesive ends. Dimers...

journal_title：Virology

authors： Freifelder D,Baran N,Folkmanis A,Freifelder DL

更新日期：1977-09-01 00:00:00

abstract：:We have characterized the fibergenes of adenovirus type 19p (Ad19p), Ad19a, and Ad37 by sequencing. The fiber genes of Ad19a and Ad37 are identical and only five amino acids differ comparing Ad19a/Ad37 with Ad19p. Based on the translated sequences we calculated the isoelectrical points (Ips) and found that the fiber k...

journal_title：Virology

authors： Arnberg N,Mei Yf,Wadell G

更新日期：1997-01-06 00:00:00

abstract：:The primary cell types that sustain polyomavirus (Py) replication following intranasal infection as well as the nature of the host cellular response to Py were unknown. As this is an essential and specific site for virus entry, it seems likely that viral gene function must be adapted to these mucosal tissues. Using im...

journal_title：Virology

authors： Gottlieb K,Villarreal LP

更新日期：2000-01-05 00:00:00

abstract：:A new type of retrovirus (Sm-MTV) released by cultured cells of a spontaneous mammary tumor from a house musk shrew, Suncus murinus, is described. The Sm-MTV is distinct morphologically from type C particles. In spite of certain morphological similarities to type B and type D retroviruses, the Sm-MTV is readily distin...

journal_title：Virology

authors： Tsutsui Y,Nagayoshi S,Saga S,Takahashi M,Aoyama A,Malavasi JY,Hayakawa S,Yokoi T,Oda S,Hoshino M

更新日期：1985-07-15 00:00:00

abstract：:A sensitive and efficient system for the functional assay of antisense oligonucleotides (oligos) was developed based on an in vitro viral assembly system. A 120-base RNA (pRNA), which indispensably participates in bacteriophage phi 29 DNA packaging, was the target for antisense action. Antisense oligos bound to pRNA, ...

journal_title：Virology

authors： Zhang C,Garver K,Guo P

更新日期：1995-08-20 00:00:00

abstract：:The presence of a genome-linked protein (VPg) at the RNA 5'-end of the genome is a characteristic of different groups of animal and plant positive-sense single-stranded RNA viruses. These viruses express their structural and functional proteins from polyproteins that are sequentially processed by at least one viral pr...

journal_title：Virology

authors： Margis R,Viry M,Pinck M,Bardonnet N,Pinck L

更新日期：1994-04-01 00:00:00

abstract：:The SARS-coronavirus (SARS-CoV) is the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV spike (S) glycoprotein mediates membrane fusion events during virus entry and virus-induced cell-to-cell fusion. To delineate functional domains of the SARS-CoV S glycoprotein, single point mutations, clu...

journal_title：Virology

authors： Petit CM,Melancon JM,Chouljenko VN,Colgrove R,Farzan M,Knipe DM,Kousoulas KG

更新日期：2005-10-25 00:00:00

abstract：:Hepatitis delta virus (HDV) encodes only one protein, the hepatitis delta antigen (HDAg). Two forms of HDAg, a large (27 kDa) and a small (24 kDa) one, participate in the various steps of HDV replication. To further understand the properties of HDAg, we have constructed recombinant baculoviruses and expressed both for...

journal_title：Virology

authors： Hwang SB,Lee CZ,Lai MM

更新日期：1992-09-01 00:00:00

abstract：:We established the transcription map of early region (E) 3 of bovine adenovirus 3 (BAV-3) by Northern blot, S1 nuclease protection assays, cDNA sequencing, and RT-PCR analysis. Five major classes of mRNAs were identified, which shared the 3' ends. Four classes of mRNAs transcribed from the E3 promoter also shared the ...

journal_title：Virology

authors： Idamakanti N,Reddy PS,Babiuk LA,Tikoo SK

更新日期：1999-04-10 00:00:00

abstract：:Efferocytosis, the phagocytic clearance of apoptotic cells, can provide host protection against certain types of viruses by mediating phagocytic clearance of infected cells undergoing apoptosis. It is known that HIV-1 induces apoptosis and HIV-1-infected cells are efferocytosed by macrophages, although its molecular m...

journal_title：Virology

authors： Chua BA,Ngo JA,Situ K,Ramirez CM,Nakano H,Morizono K

更新日期：2018-02-01 00:00:00

abstract：:The envelope glycoprotein (Env) of Moloney murine leukemia virus (MoMuLV) is proteolytically processed and transported to the cell surface where it can be incorporated into budding virions. Cell surface Env is frequently detected using an indirect immunofluorescence assay and fluorescence-activated cell sorting (FACS)...

journal_title：Virology

authors： Yu H,Empig C,Xia J,Anderson WF

更新日期：1998-04-10 00:00:00

abstract：:Human cytomegalovirus (HCMV) glycoprotein B (gB) promotes virion entry into cells by fusing the virion envelope with the cellular membrane. We recently reported that UB cells (U373 glioblastoma cells constructed to produce HCMV gB constitutively) form multinucleate syncytia that are dependent on the density of gB in t...

journal_title：Virology

authors： Tugizov S,Wang Y,Qadri I,Navarro D,Maidji E,Pereira L

更新日期：1995-06-01 00:00:00

abstract：:Infection with herpes simplex virus leads to amplification of SV40 DNA in various SV40-transformed cells. In earlier studies with the SV40-transformed hamster cell line Elona two different types of DNA amplification could be identified: (i) Bidirectional overreplication of chromosomally integrated SV40 DNA expanding i...

journal_title：Virology

authors： Gerspach R,Matz B

更新日期：1989-12-01 00:00:00

abstract：:Two small viral proteins (DGBp1 and DGBp2) have been proposed to act in a concerted manner to aid intra- and intercellular trafficking of carmoviruses though the distribution of functions and mode of action of each protein partner are not yet clear. Here we have confirmed the requirement of the DGBps of Pelargonium fl...

journal_title：Virology

authors： Martínez-Turiño S,Hernández C

更新日期：2011-05-10 00:00:00

abstract：:Infections of nonpermissive ( sup0 ) Escherichia coli by T1 phage with amber mutations in either gene 3.5 or gene 4 exhibit a variety of defective phenotypes, including premature arrest of T1 DNA synthesis, failure to make concatemeric DNA, formation of an abnormal DNA replication intermediate, failure to package phag...

journal_title：Virology

authors： Pugh JC,Ritchie DA

更新日期：1984-05-01 00:00:00

abstract：:Studies were performed to induce cross-clade neutralizing antibodies (Abs) by testing various combinations of prime and boost constructs that focus the immune response on structurally-conserved epitopes in the V3 loop of HIV-1 gp120. Rabbits were immunized with gp120 DNA containing a V3 loop characterized by the GPGR ...

journal_title：Virology

authors： Zolla-Pazner S,Cohen S,Pinter A,Krachmarov C,Wrin T,Wang S,Lu S

更新日期：2009-09-15 00:00:00