Quantitation of MoMuLV envelope protein on the cell surface.

abstract：:The OC43 strain of human coronavirus (HCoV-OC43) is an ubiquitous respiratory tract pathogen possessing neurotropic capacities. Coronavirus structural envelope (E) protein possesses specific motifs involved in protein-protein interaction or in homo-oligomeric ion channel formation, which are known to play various role...

journal_title：Virology

authors： Stodola JK,Dubois G,Le Coupanec A,Desforges M,Talbot PJ

更新日期：2018-02-01 00:00:00

abstract：:The differential symptom determinants of the Holmes' masked (M) and U1 strains of tobacco mosaic virus previously were mapped to the 5'-coterminal open reading frame (ORF) encoding the 126-kDa protein and the N-terminal two-thirds of the 183-kDa protein. Both proteins influence viral RNA accumulation, but the function...

journal_title：Virology

authors： Shintaku MH,Carter SA,Bao Y,Nelson RS

更新日期：1996-07-01 00:00:00

abstract：:Whole genome sequences for three Epstein-Barr virus strains (B95-8, GD1 and AG876) were aligned and compared. In addition to known variable loci (including type-specific alleles for the EBNA2, EBNA3A, EBNA3B and EBNA3C genes, plus the EBNA1 and LMP1 genes), seven large-scale regions of lower-level diversity were ident...

journal_title：Virology

authors： McGeoch DJ,Gatherer D

更新日期：2007-03-01 00:00:00

abstract：:We have quantitated replication of the RNA genomes of vesicular stomatitis virus (VSV) and its defective interfering (DI) particles in a BHK21 cell-free system into which nucleocapsids were introduced in varying amounts and ratios, with or without addition of purified virus polymerase components. The quantitative tran...

journal_title：Virology

authors： Giachetti C,Holland JJ

更新日期：1989-05-01 00:00:00

abstract：:To identify ranavirus virulence genes, we engineered Frog Virus 3 (FV3) knockout (KO) mutants defective for a putative viral caspase activation and recruitment domain-containing (CARD) protein (Δ64R-FV3) and a β-hydroxysteroid dehydrogenase homolog (Δ52L-FV3). Compared to wild type (WT) FV3, infection of Xenopus tadpo...

journal_title：Virology

authors： Andino Fde J,Grayfer L,Chen G,Chinchar VG,Edholm ES,Robert J

更新日期：2015-11-01 00:00:00

abstract：:Computational analysis of human adenovirus type 4 (HAdV-E4), a pathogen that is the only HAdV member of species E, provides insights into its zoonotic origin and molecular adaptation. Its genome encodes a domain of the major capsid protein, hexon, from HAdV-B16 recombined into the genome chassis of a simian adenovirus...

journal_title：Virology

authors： Dehghan S,Seto J,Liu EB,Walsh MP,Dyer DW,Chodosh J,Seto D

更新日期：2013-09-01 00:00:00

abstract：:The satellite RNA of Bamboo mosaic virus (satBaMV) contains on open reading frame for a 20-kDa protein that is flanked by a 5'-untranslated region (UTR) of 159 nucleotides (nt) and a 3'-UTR of 129 nt. A secondary structure was predicted for the 5'-UTR of satBaMV RNA, which folds into a large stem-loop (LSL) and a smal...

journal_title：Virology

authors： Annamalai P,Hsu YH,Liu YP,Tsai CH,Lin NS

更新日期：2003-06-20 00:00:00

abstract：:H7N9 influenza A first caused human infections in early 2013 in China. Virus genetics, histories of patient exposures to poultry, and previous experimental studies suggest the source of the virus is a domestic avian species, such as chickens. In order to better understand the ecology of this H7N9 in chickens, we evalu...

journal_title：Virology

authors： Spackman E,Pantin-Jackwood M,Swayne DE,Suarez DL,Kapczynski DR

更新日期：2015-03-01 00:00:00

abstract：:An in-frame gag gene mutant of human immunodeficiency virus type 1, which carries two amino acid substitutions in the center of the p24 coding region, was constructed in vitro, and its replication properties in several cell lines were examined. In CD4-negative SW480 cells transfected with the mutant clone, synthesis a...

journal_title：Virology

authors： Sakuragi J,Sakai H,Kawamura M,Tokunaga K,Ueda S,Adachi A

更新日期：1995-09-10 00:00:00

abstract：:Long-term persistent infections were established with the flavivirus, West Nile virus (WNV), strain E101, in embryofibroblast cultures derived from susceptible C3H/HE and congenic-resistant C3H/RV mice. Cultures were initially maintained by weekly subculture at 37 degrees, but at passage 6 sister cultures were shifted...

journal_title：Virology

authors： Brinton MA,Davis J,Schaefer D

更新日期：1985-01-15 00:00:00

abstract：:In an attempt to develop an in vitro human cytomegalovirus (HCMV) latency model system, the growth characteristics of HCMV in a human thyroid papillary carcinoma cell line (TPC-1) were examined. When TPC-1 cultures preheated at 40.5 degrees for 48 hr were infected with HCMV and incubated at a supraoptimal temperature ...

journal_title：Virology

authors： Tanaka J,Ogura T,Sato H,Hatano M

更新日期：1987-11-01 00:00:00

abstract：:Many tumors, including cervical carcinoma, show dysregulated expression of the microRNA processing machinery, specifically DROSHA and DICER. Some cervical cancers exhibit chromosome 5p amplifications and DROSHA is the most significantly upregulated transcript and is observed in all tumors with 5p gain. DROSHA and DICE...

journal_title：Virology

authors： Harden ME,Munger K

更新日期：2017-07-01 00:00:00

abstract：:A series of carboxy- and amino-terminal deletion mutations in the movement protein (MP) gene of tobacco mosaic virus (TMV) were ligated into a cloned TMV cDNA deleted for the endogenous MP gene. RNA transcripts were produced in vitro from clones carrying the various mutated MP genes. The effect of the deletion mutatio...

journal_title：Virology

authors： Gafny R,Lapidot M,Berna A,Holt CA,Deom CM,Beachy RN

更新日期：1992-04-01 00:00:00

abstract：:Circoviruses are relatively novel pathogens with increased importance in canids. In this study, we first screened the presence of dog circovirus (DogCV) by molecular methods from a total number of 389 internal organ samples originating from 277 individuals of domestic dogs and wild animals including wolves, foxes and ...

journal_title：Virology

authors： Zaccaria G,Malatesta D,Scipioni G,Di Felice E,Campolo M,Casaccia C,Savini G,Di Sabatino D,Lorusso A

更新日期：2016-03-01 00:00:00

abstract：:We test the hypothesis that the translation machinery in cells infected by influenza A virus efficiently translates only mRNAs that possess the influenza viral 5' untranslated region (5'-UTR) by introducing mRNAs directly into the cytoplasm of infected cells. This strategy avoids effects due to the inhibition of the n...

journal_title：Virology

authors： Cassetti MC,Noah DL,Montelione GT,Krug RM

更新日期：2001-10-25 00:00:00

abstract：:We recently developed an in vitro transcapsidation system in which infectivity of single-shelled (ss) rotavirus particles was successfully rescued (Chen and Ramig, Virology [1993]). Here, we report the rescue of infectivity of rotavirus core particles using virus strain B223 (G serotype 10) as the core donor and strai...

journal_title：Virology

authors： Chen D,Ramig RF

更新日期：1993-06-01 00:00:00

abstract：:Two tick-borne encephalitis (TBE) virus variants were studied: mouse brain-adapted strain EK-328 and its derivate adapted to Hyalomma marginatum ticks. The tick-adapted virus exhibited small-plaque phenotype and slower replication in PEK cells, higher yield in ticks, decreased neuroinvasiveness in mice, increased bind...

journal_title：Virology

authors： Romanova LIu,Gmyl AP,Dzhivanian TI,Bakhmutov DV,Lukashev AN,Gmyl LV,Rumyantsev AA,Burenkova LA,Lashkevich VA,Karganova GG

更新日期：2007-05-25 00:00:00

abstract：:Avarol is a sesquiterpenoid hydroquinone, which displays no inhibitory potencies on mammalian DNA polymerases alpha, beta, and gamma, on mammalian RNA polymerases I, II, and III, or on reverse transcriptases from Moloney murine leukemia virus (Mo-MuLV) and from HIV. For a further elucidation of the antiviral effect of...

journal_title：Virology

authors： Kuchino Y,Nishimura S,Schröder HC,Rottmann M,Müller WE

更新日期：1988-08-01 00:00:00

abstract：:The genome of the Bacillus subtilis phage SP01 contains a 12.6-kilobase-pair terminal redundancy. The transcription units in this region, which are utilized in vitro by B. subtilis RNA polymerase holoenzme, have been mapped. In vitro transcripts were separated and characterized by gel electrophoresis. Since there is s...

journal_title：Virology

authors： Brennan SM,Chelm BK,Romeo JM,Geiduschek EP

更新日期：1981-06-01 00:00:00

abstract：:The Fgf-3 protooncogene (previously called int-2) is a target of proviral insertion mutations in mammary tumors induced by the mouse mammary tumor virus (MMTV). These insertion mutations result in the transcriptional activation of Fgf-3, which is not normally expressed in the adult mammary gland. Previous mapping stud...

journal_title：Virology

authors： Morris DW,Dutra JC

更新日期：1997-11-10 00:00:00

abstract：:The major late promoter (MLP) of the subgroup C human adenoviruses is a preeminent model for the study of the mechanisms of basal and activated transcription, both in vivo and in vitro. However, while the structure and function of the human virus MLP has been the subject of extensive investigation, the conservation of...

journal_title：Virology

authors： Song B,Hu SL,Darai G,Spindler KR,Young CS

更新日期：1996-06-15 00:00:00

abstract：:Pre-exposure prophylaxis (PrEP) strategies utilizing anti-retroviral drugs show considerable promise for HIV prevention. However there is insufficient pharmacokinetic (PK) data on drug concentrations required for protection at the relevant mucosal tissues where the infection is initiated. Here we evaluated the utility...

journal_title：Virology

authors： Veselinovic M,Yang KH,LeCureux J,Sykes C,Remling-Mulder L,Kashuba ADM,Akkina R

更新日期：2014-09-01 00:00:00

abstract：:The requirement to replicate in both vertebrate and invertebrate hosts is thought to limit the introduction of genetic changes into the genome of arboviruses. Serial passage under laboratory conditions will overcome this limitation allowing for genetic changes to be introduced and affecting the virulence of the virus ...

journal_title：Virology

authors： McCurdy K,Joyce J,Hamilton S,Nevins C,Sosna W,Puricelli K,Rayner JO

更新日期：2011-06-20 00:00:00

abstract：:Tombusviruses, of which tomato bushy stunt virus (TBSV) is the type member, spontaneously generate defective interfering RNAs (DIs) that are known to interfere with viral accumulation and symptom development. We show that co-infection with TBSV and DIs causes a dramatic reduction in accumulation of TBSV subgenomic RNA...

journal_title：Virology

authors： Scholthof KB,Scholthof HB,Jackson AO

更新日期：1995-08-01 00:00:00

abstract：:Multiplication of frog virus 3 (FV 3) occurs in mammalian cells provided they are incubated at temperatures lower than 33 degrees. The expression of the viral genome at supraoptimal temperatures was followed by analyzing the polypeptides produced in CHO-infected cells and comparing with those obtained under restrictiv...

journal_title：Virology

authors： Cordier O,Tondre L,Aubertin AM,Kirn A

更新日期：1986-07-30 00:00:00

abstract：:The major outer capsid protein, VP5, of five US bluetongue viruses, was found to be glycosylated. Enzymatic removal of the carbohydrate moiety did not affect the electrophoretic mobility of VP5 in SDS-PAGE, indicating the presence of only short and possibly unbranched oligosaccharide chains. The surface accessibilitie...

journal_title：Virology

authors： Yang YY,Li JK

更新日期：1993-05-01 00:00:00

abstract：:Structure-guided approaches to HIV-1 vaccine design depend on knowledge of the presentation of neutralizing epitopes on gp120, such as the epitope for the broadly neutralizing mAb b12. Here, we characterized predicted three-dimensional structures of functionally diverse gp120 proteins in their b12-bound conformation, ...

journal_title：Virology

authors： Sterjovski J,Churchill MJ,Ellett A,Wesselingh SL,Ramsland PA,Gorry PR

更新日期：2012-10-25 00:00:00

abstract：:We report the construction of herpes simplex virus 1 recombinants from which all or part of the coding sequences of four open reading frames have been deleted. In recombinants R7101 and R7108, the BamHI D' fragment containing the coding sequences of the dUTPase gene and the promoter-regulatory domain of a late gene wa...

journal_title：Virology

authors： Barker DE,Roizman B

更新日期：1990-08-01 00:00:00

abstract：:The utilization of transgenic plants expressing recombinant antigens to be used in the formulation of experimental immunogens has been recently communicated. We report here the development of transgenic plants of alfalfa expressing the structural protein VP1 of foot and mouth disease virus (FMDV). The presence of the ...

journal_title：Virology

authors： Wigdorovitz A,Carrillo C,Dus Santos MJ,Trono K,Peralta A,Gómez MC,Ríos RD,Franzone PM,Sadir AM,Escribano JM,Borca MV

更新日期：1999-03-15 00:00:00

abstract：:SV(PZF) is a mutant of Sindbis virus (SV) which we selected on the basis of its ability to replicate in mosquito cells treated with pyrazofurin (PZF), a drug which inhibits pyrimidine nucleotide biosynthesis (Lin et al., 2000, Virology 272, 61-71). Three mutations, A6627U, A7543U, and C7593A, were identified in the ns...

journal_title：Virology

authors： Lin YH,Simmonds HA,Stollar V

更新日期：2002-01-05 00:00:00