Abstract:
:In an attempt to develop an in vitro human cytomegalovirus (HCMV) latency model system, the growth characteristics of HCMV in a human thyroid papillary carcinoma cell line (TPC-1) were examined. When TPC-1 cultures preheated at 40.5 degrees for 48 hr were infected with HCMV and incubated at a supraoptimal temperature (40.5 degrees), the cultures could be maintained for at least 65 days without detection of infectious virus. In contrast, when the infected cultures were incubated at 37 degrees, HCMV persistently infected cultures were established. HCMV was reactivated from the latently infected cultures by decreasing the incubation temperature from 40.5 to 37 degrees, and the cultures subsequently entered into virus persistent infection. Although HCMV-specific polypeptides which comigrate with the immediate early virus polypeptides and nuclear antigens were continuously detectable in the majority (more than 95%) of the cells during the latent period, a detectable level of virus-specified DNA polymerase (one of the early virus proteins) was not induced, suggesting that the blockage of HCMV replication in the latently infected cultures occurs at the early stages of the HCMV replication cycle. Infectious center assay revealed that 0.002 to 0.2% of the cells contain an HCMV genome that can be activated during the latent period. The latently infected cells were susceptible to superinfection with homologous and heterologous strains of HCMV. In persistently infected cultures approximately 38% of the cells were lysed by reaction with HCMV immune serum and complement, whereas complement-mediated immune cytolysis could not be detected in the latently infected cultures. The data presented suggest that a temperature-sensitive cellular function(s) that controls the expression of the HCMV early functions plays an important role in maintenance of the HCMV genome in the latent state and reactivation of HCMV by decreasing the incubation temperature.
journal_name
Virologyjournal_title
Virologyauthors
Tanaka J,Ogura T,Sato H,Hatano Mdoi
10.1016/0042-6822(87)90171-1subject
Has Abstractpub_date
1987-11-01 00:00:00pages
62-72issue
1eissn
0042-6822issn
1096-0341journal_volume
161pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Human rotaviruses (Wa, KUN, MO) showed hemagglutination (HA) only with fixed 1-day-old chicken erythrocytes, and their HA activities were completely destroyed by trypsin activation of virions. Simian SA-11 and bovine NCDV had HA activities not only against fixed erythrocytes but also against fresh erythrocytes from va...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90121-9
更新日期:1989-09-01 00:00:00
abstract::A quantitative analysis of Epstein-Barr virus (EBV)-induced early antigen (EA) and viral capsid antigen (VCA) syntheses was carried out in Raji cells superinfected with purified, concentrated P3HR-1 EBV. When the cells were exposed to the virus and assessed by immunofluorescence and immunoprecipitation, EA induction o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90276-3
更新日期:1983-07-30 00:00:00
abstract::The polyomavirus hr-t class of mutants has served as a major prototype to study the function of middle T + small T in the virus lytic cycle, Biochem. Biophys. Acta 695 (2), 69-95). The properties of these middle T + small T defective mutants were defined by comparisons with "wild-type" strains reconstructed by marker ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00030-2
更新日期:2003-03-15 00:00:00
abstract::Simian-human immunodeficiency virus encoding both reverse transcriptase (RT) and envelope genes of HIV-1 (RT Env SHIV) is important for evaluating biomedical prevention modalities for HIV/AIDS. We describe virological characterization of a clade B RT Env SHIV following infection of macaques via multiple routes. In viv...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.05.034
更新日期:2012-10-10 00:00:00
abstract::Attempts to define the genetic determinants required for efficient growth of human immunodeficiency virus type 1 (HIV-1) in monocyte-macrophages were made by constructing chimeras between two infectious clones of HIV-1 (HXB2 and LW/C), which despite only minor differences in their DNA sequence have striking difference...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80082-4
更新日期:1995-01-10 00:00:00
abstract::An in-frame gag gene mutant of human immunodeficiency virus type 1, which carries two amino acid substitutions in the center of the p24 coding region, was constructed in vitro, and its replication properties in several cell lines were examined. In CD4-negative SW480 cells transfected with the mutant clone, synthesis a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1478
更新日期:1995-09-10 00:00:00
abstract::The Tat regulatory protein encoded by human immunodeficiency virus type 1 (HIV-1) induces high levels of transcription from the viral long terminal repeat (LTR) promoter element after interacting with a promoter proximal RNA target sequence. In the wild-type HIV-1 LTR, this activation is facilitated by the synergistic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1041
更新日期:1995-02-01 00:00:00
abstract::Ubiquitin C-terminal Hydrolase L1 (UCH-L1) has oncogenic properties and is highly expressed during malignancies. We recently documented that Epstein-Barr virus (EBV) infection induces uch-l1 expression. Here we show that Kaposi's Sarcoma-associated herpesvirus (KSHV) infection induced UCH-L1 expression, via cooperatio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.10.018
更新日期:2014-01-05 00:00:00
abstract::The spread of HIV-1 to the nervous system occurs early during infection in a large number of asymptomatic virus carriers. In order to address the question whether the brain is targeted by a group of HIV-1 variants with increased neurotropic properties we have obtained 20 paired isolates from the blood and cerebrospina...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1503
更新日期:1993-10-01 00:00:00
abstract::Mammalian type C retroviruses can be detected by their induction of foci of cell transformation in S+I - cells. We have noted that certain subtypes of the mouse xenotropic type C retrovirus (MuLV) inhibit this cell alteration. This inhibition, associated with intact virions, is irreversible and gives the infected cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90044-4
更新日期:1987-09-01 00:00:00
abstract::The simian hemorrhagic fever virus (SHFV) genome differs from those of other members of the family Arterivirus in encoding two adjacent sets of four minor structural protein open reading frames (ORFs). A stable, full-length, infectious SHFV-LVR cDNA clone was constructed. Virus produced from this clone had replication...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.06.001
更新日期:2014-08-01 00:00:00
abstract::Several purified species of human leukocyte interferon, including recombinant interferons, inhibit the multiplication of tobacco mosaic virus (TMV) in tobacco protoplasts derived from various cultivars. Viral RNA accumulation was determined by dot-blot hybridization to specific cDNA probes, and virus antigen was deter...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90456-8
更新日期:1985-01-15 00:00:00
abstract::HAV particles isolated from infected cells banded at buoyant densities of 1.42, 1.32, and 1.20 g/ml, and distinctive protein patterns were established by gel electrophoresis and reverse phase high performance liquid chromatography. The relatively higher amounts of p30 in particles with lower buoyant densities suggest ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90234-5
更新日期:1986-12-01 00:00:00
abstract::Rotaviral RNA was detected in the stool sample of an asymptomatic fattening pig at a Slovenian pig farm. To characterize the rotavirus, RT-PCR was used, employing primers specific for the VP7, VP4 and NSP4 genes. Specific products were purified and the sequencing reaction was performed for the molecular analysis of am...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.001
更新日期:2007-03-15 00:00:00
abstract::We report herein that vesicular stomatitis virus (VSV) induced a concurrent primary Th1 (T helper 1) and Th2 cytokine response detectable ex vivo. Liposome-encapsulated clodronate-mediated elimination of CD8- marginal dendritic cells (DCs) and splenic macrophages (m Phi), but not CD8+ interdigitating DCs, prior to inf...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.07.031
更新日期:2005-11-25 00:00:00
abstract::Gammaherpesviruses establish life-long infection in most adults and are associated with the development of B cell lymphomas. While the interaction between gammaherpesviruses and splenic B cells has been explored, very little is known about gammaherpesvirus infection of B-1 B cells, innate-like B cells that primarily r...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.02.022
更新日期:2016-05-01 00:00:00
abstract::Red sea bream iridovirus (RSIV) of the Iridoviridae family is a causative agent of lethal infections in many cultured marine fish species in southwestern Japan. RSIV-induced apoptosis was divided as follows: (1). cell shrinkage and rounding at the early apoptotic stage, (2). cell enlargement at the middle apoptotic st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.02.006
更新日期:2004-05-01 00:00:00
abstract::From previously constructed genomic libraries of a Taiwan WSSV isolate, a putative WSSV tk-tmk gene was identified. Uniquely, the open reading frame (ORF) of this gene was predicted to encode a novel chimeric protein of 388 amino acids with significant homology to two proteins: thymidine kinase (TK) and thymidylate ki...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0597
更新日期:2000-11-10 00:00:00
abstract::A central, yet unresolved issue in the pathogenesis of HIV disease is the mechanism of antibody perturbation. In this study, HIV-specific memory B-cells were quantified in groups of infected subjects and compared with memory responses to other antigens and antibody titers. HIV-specific memory B-cell responses were vig...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.003
更新日期:2010-02-05 00:00:00
abstract::A cDNA was constructed to encode an internally-truncated version of negative-sense genomic RNA (vRNA) of respiratory syncytial virus (RSV) containing (in 3' to 5' order) the 3' vRNA leader region, the complement of the open reading frame for bacterial chloramphenicol acetyl transferase (CAT) under the control of RSV g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1368
更新日期:1993-07-01 00:00:00
abstract::During head assembly of phage T3, DNA is packaged into the cavity of a preformed protein shell, called the prohead, with the aid of noncapsid, packaging proteins, the products of genes 18 and 19 (gp18 and gp19). gp18 and gp19 separately form complexes with DNA and proheads, respectively. These complexes associate to f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90550-u
更新日期:1991-12-01 00:00:00
abstract::Next generation sequence analyses were used to assess virus-derived small RNA (vsRNA) profiles for Homalodisca coagulata virus-1 (HoCV-1), family Dicistroviridae, and Homalodisca vitripennis reovirus (HoVRV), family Reoviridae, from virus-infected H. vitripennis, the glassy-winged sharpshooter. The vsRNA reads were ma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.04.005
更新日期:2013-07-20 00:00:00
abstract::Using a defective interfering Sendai virus stock (DIH4) freed of nondefective helper virus, we found that the closely related parainfluenza viruses 1 and 3 could substitute for the Sendai virus helper in replicating DIH4, creating chimeric nucleocapsids. The morbillivirus measles and the rhabdovirus VSV could not subs...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90660-4
更新日期:1991-05-01 00:00:00
abstract::The v-Rel oncoprotein of the Rev-T retrovirus interacts with a number of cellular proteins in transformed chicken spleen cells including p40/I kappa B alpha, p68c-Rel, hsc70, and the p124 and p115 precursors for the p50 and p52 subunits of NF kappa B. Here we report that v-Rel associates with at least three other cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1095
更新日期:1995-03-10 00:00:00
abstract::We have carried out an exhaustive search for amino acid sequence similarities between vesicular stomatitis virus (VSV) proteins and database entries. Unexpectedly, we found that the L polymerase protein contains two blocks of sequence (residues 725-1102 and 1291-1671) with distant but statistically significant similar...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90148-7
更新日期:1989-09-01 00:00:00
abstract::The expression of cytokines may influence the development of lymphoma in retrovirally infected animals in at least two ways: (1) cytokines in the tumor environment may stimulate the proliferation of tumor cells and/or (2) cytokines in the tumor environment may diminish the cell-mediated antitumor immune response. To e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9846
更新日期:1999-09-01 00:00:00
abstract::Cauliflower mosaic virus (CaMV) strains CM1841 and W260 produced markedly different symptoms when inoculated onto turnips (Brassica campestris L. 'Just Right'). The CM1841 strain induced a mild degree of stunting of infected plants while strain W260 caused moderate to severe stunting. Although CM1841 was significantly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90898-l
更新日期:1991-04-01 00:00:00
abstract::By employing two different buffer solutions for polyacrylamide gel electrophoresis, all genome segments of Fiji disease and maize rough dwarf virus were separated. Fiji disease virus contains ten genome segments with approximate genome molecular weights of 19.26 x 10(6) and 19.85 x 10(6), depending on the buffer emplo...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::Human keratinocytes (HKc) immortalized by human papillomavirus type 16 DNA (HKc/HPV16) progress toward malignancy through growth factor-independent (HKc/GFI) and differentiation-resistant stages (HKc/DR). This progression is associated with a loss of sensitivity to growth inhibition by both all-trans-retinoic acid (RA...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0283
更新日期:2000-05-10 00:00:00
abstract::The HIV-1 protein Vpr enhances macrophage infection, triggers G2 cell cycle arrest, and targets cells for NK-cell killing. Vpr acts through the CRL4(DCAF1) ubiquitin ligase complex to cause G2 arrest and trigger expression of NK ligands. Corresponding ubiquitination targets have not been identified. UNG2 and SMUG1 are...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.06.010
更新日期:2013-09-01 00:00:00