Abstract:
:From previously constructed genomic libraries of a Taiwan WSSV isolate, a putative WSSV tk-tmk gene was identified. Uniquely, the open reading frame (ORF) of this gene was predicted to encode a novel chimeric protein of 388 amino acids with significant homology to two proteins: thymidine kinase (TK) and thymidylate kinase (TMK). Northern blot analysis with a WSSV tk-tmk-specific riboprobe detected a major transcript of 1.6 kb. When healthy adult Penaeus monodon shrimp were inoculated with WSSV, the tk-tmk gene transcript was first detected by RT-PCR analysis at 4 h postinfection and transcription levels continued to increase over the first 18 h. The gene's major in vitro transcription and translation product, equivalent to the predicted size (43 kDa), is a single chimeric protein that includes both the TK and TMK functional motifs. Evidence for phylogenetic analysis and sequence alignment suggested that the gene may have resulted from the fusion of a cellular-type TK gene and a cellular-type TMK gene. Its unique arrangement may also provide a valuable gene marker for WSSV.
journal_name
Virologyjournal_title
Virologyauthors
Tsai MF,Yu HT,Tzeng HF,Leu JH,Chou CM,Huang CJ,Wang CH,Lin JY,Kou GH,Lo CFdoi
10.1006/viro.2000.0597subject
Has Abstractpub_date
2000-11-10 00:00:00pages
100-10issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(00)90597-Xjournal_volume
277pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Equine influenza is caused by two serotypes of type A influenza virus, EIV-A1 and EIV-A2. The complete nucleotide sequence of the neuraminidase (NA) genes of both the A1 (N7 subtype) and A2 (N8 subtype) serotype has been determined following cloning of full-length viral NA cDNAs into pBR322. Analysis of the deduced am...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90207-2
更新日期:1986-12-01 00:00:00
abstract::A cDNA clone containing all of the 26 S mRNA coding region of the RNA genome of Venezuelan equine encephalitis (VEE) virus, virulent strain Trinidad donkey (TRD), has been constructed and sequenced. The nucleotide and deduced amino acid sequences of the 26 S RNA of VEE virus conform to the general organization of the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90142-x
更新日期:1986-07-30 00:00:00
abstract::The mechanism of intracellular retention for the large surface protein (L) of duck hepatitis B virus (DHBV) was analyzed by examination of the transmembrane topologies and secretory properties of a collection of DHBV L mutants and compared with that of human hepatitis B virus (HBV) L. Our results demonstrate that, in ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.9015
更新日期:1998-03-15 00:00:00
abstract::An infectious raccoon poxvirus (RCNV) was used to express the feline panleukopenia virus (FPV) open reading frame VP2. The recombinant, RCNV/FPV, was constructed by homologous recombination with a chimeric plasmid for inserting the expression cassette into the thymidine kinase (TK) locus of RCNV. Expression of the VP2...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0186
更新日期:1996-04-01 00:00:00
abstract::Several sulfated polysaccharides (dextran sulfate, pentosan polysulfate, heparin) and copolymers of acrylic acid with vinylalcohol sulfate have proved to be potent inhibitors of human cytomegalovirus (CMV) infectivity in vitro. Sulfated alpha-cyclodextrins are only weak inhibitors of CMV. A close correlation was found...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90680-n
更新日期:1992-07-01 00:00:00
abstract::Deletion of the nef gene results in viral attenuation and confers protection against challenge with wild-type simian immunodeficiency virus in macaques. Regarding HIV-1 infection, a few long-term nonprogressors (LTNP) with nef deletions have been described. In this study, the nef genes of a group of seven LTNP and eig...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9783
更新日期:1999-07-05 00:00:00
abstract::Studies using transformed human cell lines suggest that most SIV strains use CCR5 as co-receptor. Our analysis of primary rhesus macaque CD4(+) T-cell clones revealed marked differences in susceptibility to SIV(mac)239 infection. We investigated whether different levels of CCR5 expression account for clonal difference...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.10.005
更新日期:2011-01-05 00:00:00
abstract::We present a case of sexual transmission of HIV-1 predicted to have CXCR4-tropism during male-to-male sexual exposure. Phylogenetic analyses exclude cell-free virus in the seminal plasma of the source partner and possibly point to the seminal cells as the origin of the transmission event. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.09.010
更新日期:2012-12-05 00:00:00
abstract::Seven structural component proteins of a paramyxovirus simian virus 41 (SV41) were identified with the aid of monoclonal antibodies prepared against SV41 and human parainfluenza type 2 virus (PIV2). The nucleoprotein is antigenically very close to that of PIV2, while it is comparatively far from that of simian virus 5...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90141-d
更新日期:1990-12-01 00:00:00
abstract::A natural variation is observed at position 4 of the 3'-end of influenza A virus genomes, where U (U4) or C (C4) is present. The replicon activity of C4 was 28% of U4. We compared the transcription and replication activity of U4 [v84(U4)], C4 [v84(C4)] and the complimentary RNA (c84) using the purified influenza virus...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.09.022
更新日期:2010-12-20 00:00:00
abstract::West Nile virus is a mosquito-borne, neurotropic flavivirus that causes encephalitis in humans and animals. Since being introduced into the Western hemisphere in 1999, WNV has spread rapidly across North America, identifying this virus as an important emerging pathogen. In this study, we developed a DNA-launched infec...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.021
更新日期:2005-03-30 00:00:00
abstract::The target for neutralizing antibodies against human immunodeficiency virus (HIV) is the trimeric Env protein on the native virion. Conserved neutralizing epitopes of receptor binding sites are located in the recessed core of the Env protein, partially masked by glycosylations and variable loops. In this study, we hav...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.10.005
更新日期:2005-01-05 00:00:00
abstract::Efforts to generate a vaccine to prevent infection by human immunodeficiency virus (HIV) have focused on inducing neutralizing antibodies. However, cytotoxic T-lymphocyte (CTL) responses are a major immune defense mechanism required for recovery from many different virus infections. Since CTL epitopes can be defined b...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90504-i
更新日期:1992-06-01 00:00:00
abstract::Although dengue (DEN) virus is the etiologic agent of dengue fever, the most prevalent vector-borne viral disease in the world, precise information on the antigenic structure of the dengue virion is limited. We have prepared a set of murine monoclonal antibodies (MAbs) specific for the envelope (E) glycoprotein of DEN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9200
更新日期:1998-07-05 00:00:00
abstract::Aside from a common gene organization shared with other picornaviruses, hepatitis A virus (HAV) is characterized by its slow-growth phenotype, the inability to shut off host macromolecular synthesis, and, in general, lack of cytopathic (cp) effects in permissive cell cultures. Nevertheless, several cp HAV strains have...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0070
更新日期:2000-01-05 00:00:00
abstract::Hepatitis B virus (HBV) clone 4B replicated much more efficiently than clone 2A of the same genotype. Introduction of its T1753C, A1762T, G1764A, and C1766T core promoter mutations into the 2A genome greatly enhanced genome replication and suppressed HBeAg expression. Here we show that these effects are mediated by tr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.03.002
更新日期:2009-05-10 00:00:00
abstract::Many viruses, including human immunodeficiency virus type 1 (HIV-1), induce apoptosis and are affected by cellular expression of antiapoptotic genes. We sought to examine the effect of antiapoptotic gene expression on HIV replication by transfecting the promyelomonocytic cell line U937 with the bcl-xl gene to obtain c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9599
更新日期:1999-03-30 00:00:00
abstract::Despite recent progress in the development of direct-acting antivirals against hepatitis C virus (HCV), chronic HCV infection remains an important health burden worldwide. MicroRNA122 (miR122), a liver-specific microRNA (miRNA), positively regulates HCV replication, and systemic application of antisense oligonucleotid...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.05.024
更新日期:2014-08-01 00:00:00
abstract::Although it is known that Ad5-specific neutralizing antibodies (NAbs) against three major viral capsid components (hexon, penton and fiber) are generated, differences in the frequency and nature of these pre-existing NAbs remain unclear. The results emphasized the contribution of anti-fiber antibodies to Ad5 neutraliz...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.12.014
更新日期:2013-03-15 00:00:00
abstract::The complete genomes of 30 Soybean mosaic virus (SMV) isolates and strains were sequenced in this study. Together with fourteen previously reported sequences, we analyzed the genetic structure of the SMV population. Analyses of genetic diversity showed that different genomic regions of SMV are under different evolutio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.007
更新日期:2009-10-10 00:00:00
abstract::We previously provided evidence for a model of herpes simplex virus type 1 (HSV-1) entry by a cascade of interactions between components of the virion envelope and cellular plasma membrane (Fuller and Lee, 1992, J. Virol. 66, 5002-5012). In this report we have determined entry kinetics of wild-type HSV-1 into two high...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1081
更新日期:1994-02-01 00:00:00
abstract::Rift Valley fever virus-induced protein synthesis was examined by polyacrylamide gel electrophoresis and fluorography. Five virus-induced polypeptides were detected, the nucleocapsid protein N, the nucleus-associated nonstructural protein NS1, the glycoproteins G1 and G2, and a protein of molecular weight 80K. The N, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90277-0
更新日期:1984-04-15 00:00:00
abstract::Glycoprotein B (gB, gpUL55) is a major antigen for the induction of neutralizing antibodies against human cytomegalovirus, making it an attractive antigen for active and passive immunoprophylaxis. The immunodominant region on gB is the antigenic domain 1 (AD-1), a complex structure which requires a minimal linear amin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0040
更新日期:1996-02-01 00:00:00
abstract::Two mutant strains of cucumber mosaic virus (CMV) were investigated with respect to virion stability and molecular determinants of aphid vector transmission. The mutant 2A1-MT-60x, derived from the mechanically passaged wild type 2A1-AT, is poorly transmissible by the aphid Aphis gossypii and not transmissible by the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0569
更新日期:2000-10-25 00:00:00
abstract::Aster yellows virus (AYV) was recovered from hemolymph, alimentary canal, salivary glands and ovaries, but not from Malpighian tubules, mycetomes, fat body, testes, or brain of viruliferous leafhoppers, Macrosteles fascifrons (Stål). The presence of virus in the tissues was determined by injecting their extracts into ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(67)90070-0
更新日期:1967-12-01 00:00:00
abstract::It has been shown that the genomes of episomally maintained DNA viruses are tethered to host cell chromosomes during cell division, facilitating maintenance in dividing cells. The papillomavirus E2 protein serves this mechanism of viral genome persistence by simultaneously associating with chromatin and the viral geno...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.03.015
更新日期:2011-05-25 00:00:00
abstract::Langat (LGT) flavivirus, derived from infectious full-length cDNA clone 636, was investigated for its apoptotic activities in mouse neuroblastoma (Neuro-2a) and simian kidney (Vero and LLC-MK(2)) cells. The hallmark of apoptosis, cleavage of cellular DNA, was observed 48 h after infection of Vero, LLC-MK(2), and Neuro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0980
更新日期:2001-08-01 00:00:00
abstract::The physical structure of the genome of feline herpesvirus-1, a major upper respiratory tract pathogen of cats, was studied. Purified FHV-1 DNA was analyzed by restriction endonuclease and gamma 5' exonuclease digestion, blot hybridization, and electron microscopy. To facilitate further studies, nine bacteriophage clo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90439-3
更新日期:1986-10-15 00:00:00
abstract::The effect of measles virus (MV) infection on mRNA expression and protein synthesis of cytokines in human malignant glioma cell lines (D-54 and U-251) was investigated. Primary MV infections led in both cell lines to the induction of interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), interferon-beta (IFN-beta), and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1363
更新日期:1993-07-01 00:00:00
abstract::Recombination is a driving force for the emergence, evolution and virulence/epidemics of viruses, comprising the Enterovirus genus of the Picornaviridae family, important for human and animal health. By analyzing 2949 complete genomes/coding sequences, we provide a thorough and up-to-date overview of the genome-wide p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.10.006
更新日期:2019-01-02 00:00:00