Abstract:
:Long-term persistent infections were established with the flavivirus, West Nile virus (WNV), strain E101, in embryofibroblast cultures derived from susceptible C3H/HE and congenic-resistant C3H/RV mice. Cultures were initially maintained by weekly subculture at 37 degrees, but at passage 6 sister cultures were shifted to 32 degrees. Virus progeny titers were observed to increase after the shift to 32 degrees indicating the possible presence of temperature-sensitive mutants. Temperature-sensitive mutants were found to arise in cultures of both susceptible and resistant cells. However, only in the resistant cultures did temperature-sensitive virus become the majority population. Temperature-sensitive mutants did not appear to be essential for either initiation or maintenance of WNV-persistant infections. The resistant cells appear to provide an environment which is advantageous for the amplification of temperature-sensitive mutants.
journal_name
Virologyjournal_title
Virologyauthors
Brinton MA,Davis J,Schaefer Ddoi
10.1016/0042-6822(85)90454-4subject
Has Abstractpub_date
1985-01-15 00:00:00pages
152-8issue
1eissn
0042-6822issn
1096-0341journal_volume
140pub_type
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