Abstract:
:The 94-kDa virion-associated RNA-dependent RNA polymerase (RdRp) is present in infectious pancreatic necrosis virus in two forms: (i) as a free polypeptide (VP1) and (ii) as a genome linked protein (VPg) (J. G. Calvert et al., 1991, J. Gen. Virol. 72, 2563-2567). VP1 was guanylylated in vitro by incubating purified virus in the presence of [alpha2P]GTP. During further incubation in an in vitro RNA polymerase reaction mixture (in the presence of unlabeled GTP), the radiolabeled VP1-pG complex was "chased" via nascent RNA strands and replicative intermediates to a VP1-dsRNA complex. Labeled VP1-pG was recovered from the intermediate as well as from the final reaction products by digestion with RNase A and RNase V1, a dsRNA-specific nuclease. Analysis of the reaction products indicated that only the plus strands of the two genome segments were being synthesized in vitro which remained base-paired to their templates. The results suggest that in vitro transcription by the virion RdRp is primed by VP1 and then proceeds via an asymmetric, semiconservative, strand-displacement mechanism.
journal_name
Virologyjournal_title
Virologyauthors
Dobos Pdoi
10.1006/viro.1995.1125subject
Has Abstractpub_date
1995-04-01 00:00:00pages
19-25issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(85)71125-7journal_volume
208pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::In a previous paper (G. Winkler, V. B. Randolph, G. R. Cleaves, T. E. Ryan, and V. Stollar, 1988, Virology 162, 187-196) we showed that the newly synthesized dengue-2 virus nonstructural protein, NS1, exists briefly as a monomer and then undergoes dimerization. We demonstrate here that the dimerization of NS1 is assoc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90544-8
更新日期:1989-07-01 00:00:00
abstract::Given dengue virus (DENV) genome austerity, it uses cellular molecules and structures for virion entry, translation and replication of the genome. NS1 is a multifunctional protein key to viral replication and pathogenesis. Identification of cellular proteins that interact with NS1 may help in further understanding the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.05.017
更新日期:2015-10-01 00:00:00
abstract::We mutagenized and characterized a 41-kilobase-pair subgenomic cloned fragment from the unique long (UL) region of pseudorabies virus (PRV). Forty mutant clones, each carrying a single inserted oligonucleotide, were used for cotransfection with overlapping cloned viral DNA fragments. More than half of these transfecti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1242
更新日期:1994-05-01 00:00:00
abstract::The structure and function of the equine herpesvirus type 1 (EHV-1) UL34 homologous protein were characterized. A UL34 protein-specific antiserum reacted with an M(r)28,000 protein that could not be detected in purified extracellular virions. Confocal laser scanning microscopy demonstrated that UL34 reactivity mainly ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1488
更新日期:2002-09-01 00:00:00
abstract::We previously demonstrated that Bombyx mori nucleopolyhedrovirus (BmNPV) multiplication is restricted in permissive BmN-4 cells upon coinfection with Hyphantria cunea NPV (HycuNPV). Here, we show that HycuNPV-encoded hycu-ep32 gene is responsible for the restricted BmNPV multiplication in HycuNPV-coinfected BmN-4 cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.049
更新日期:2010-03-15 00:00:00
abstract::Envelope viruses maturate by macromolecule assembly and budding. To investigate these steps, we generated virus-like particles (VLPs) by co-expression of structural proteins of Sendai virus (SeV), a prototype of the family Paramyxoviridae. Simultaneous expression of matrix (M), nucleo- (N), fusion (F), and hemagglutin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.04.019
更新日期:2004-07-20 00:00:00
abstract::The potential role(s) of cytokines in the reduction of infectious virus and persistent viral infection in the central nervous system was examined by determining the kinetics of cytokine mRNA expression following infection with the neurotropic JHM strain of mouse hepatitis virus. Mice were infected with an antibody esc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8613
更新日期:1997-07-07 00:00:00
abstract::HIV-1 transcytosis has been proposed as a potential mechanism allowing the virus to cross the epithelium during mucosal transmission. Epitopes of the HIV-1 envelope involved in this process have not been identified yet. Here, we assessed a large panel of HIV neutralizing antibodies recognizing well-characterized epito...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.09.006
更新日期:2008-01-20 00:00:00
abstract::The majority of HIV-1 infections occur via sexual intercourse. Women are the most affected by the epidemic, particularly in developing countries, due to their socio-economic dependence on men and the fact that they are often victims of gender based sexual violence. Despite significant efforts that resulted in the redu...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2016.07.004
更新日期:2016-10-01 00:00:00
abstract::While DNA vaccines have been shown to prime cellular immune responses, levels are often low in nonhuman primates or humans. Hence, efforts have been directed toward boosting responses by combining DNA with different vaccination modalities. To this end, a polyvalent DNA prime/protein boost vaccine, consisting of codon ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.038
更新日期:2006-03-01 00:00:00
abstract::The murine coronavirus polymerase gene is 22 kb in length with the potential to encode a polyprotein of approximately 750 kDa. The polyprotein has been proposed to encode three proteinase domains which are responsible for the processing of the polyprotein into mature proteins. The proteolytic activity of the first pro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1567
更新日期:1994-11-01 00:00:00
abstract::Feline immunodeficiency virus (FIV) is a lentivirus associated with an immunodeficiency syndrome of the domestic cat. A short open reading frame (ORF2), of unknown function, is present in all FIV isolates. We have investigated the role of ORF2 in determining the cell tropism of two infectious molecular clones of FIV. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0002
更新日期:1996-01-01 00:00:00
abstract::The RNA genomes of cytopathogenic bovine viral diarrhea virus (BVDV) isolates contain insertions highly homologous to cellular sequences. For two of them the insert was identified as ubiquitin coding sequence. The genome of BVDV Osloss contains exactly one ubiquitin gene monomer. In the case of BVDV CP1 the cellular i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90074-l
更新日期:1991-02-01 00:00:00
abstract::Long-term persistent infections were established with the flavivirus, West Nile virus (WNV), strain E101, in embryofibroblast cultures derived from susceptible C3H/HE and congenic-resistant C3H/RV mice. Cultures were initially maintained by weekly subculture at 37 degrees, but at passage 6 sister cultures were shifted...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90454-4
更新日期:1985-01-15 00:00:00
abstract::The role of bean common mosaic necrosis potyvirus (BCMNV) and lettuce mosaic potyvirus (LMV) proteins was investigated in terms of their capacity to function as viral movement proteins (MPs). Using Escherichia coli-expressed proteins and microinjection techniques, direct evidence was obtained that both the potyviral c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8777
更新日期:1997-10-27 00:00:00
abstract::The Psu (Polarity suppression) protein of satellite bacteriophage P4 was first characterized as an anti-terminator of transcription termination in Escherichia coli. Psu is also a structural component of mature P4 capsids, where it is present as a decoration protein. Psu is located externally on the capsid surface, and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1307
更新日期:1993-06-01 00:00:00
abstract::Our previous studies have demonstrated that virus-specific cell-mediated cytotoxicity of sensitized leukocytes can be induced using clonal ginbuna crucian carp and their syngeneic cell lines. In the present study, we attempt to determine if virus-specific cytotoxic cell populations of fish express CD8alpha and TCRbeta...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.01.019
更新日期:2006-05-10 00:00:00
abstract::Certain temperature-sensitive (ts) mutants of murine leukemia virus (MuLV) were observed to be defective in virus assembly. These mutants also accumulated intracellular core protein precursor, Pr65gag, at 39 degrees, the nonpermissive temperature. At 39 degrees, virions released from cells infected with the various ts...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90306-4
更新日期:1984-04-30 00:00:00
abstract::The efficacy of combining immunization with human immunodeficiency vitus type 1 (HIV-1) DNA and HIV-1 recombinant proteins to obtain protection from chimeric simian/human immunodeficiency virus (SHIV) was determined. Four cynomolgus monkeys received four gene-gun immunizations intraepidermally of plasmid DNA encoding ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9379
更新日期:1998-10-25 00:00:00
abstract::Hepatitis C virus (HCV) causes chronic hepatitis, which often results in the development of liver cirrhosis and hepatocellular carcinoma (HCC) worldwide. In this study, we demonstrated that the non-structural protein NS3 of HCV enhances cyclooxygenase-2 (COX-2) gene promoter activity, COX-2 mRNA expression, COX-2 prot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.09.025
更新日期:2008-02-05 00:00:00
abstract::The HIV-1 protein Vpr enhances macrophage infection, triggers G2 cell cycle arrest, and targets cells for NK-cell killing. Vpr acts through the CRL4(DCAF1) ubiquitin ligase complex to cause G2 arrest and trigger expression of NK ligands. Corresponding ubiquitination targets have not been identified. UNG2 and SMUG1 are...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.06.010
更新日期:2013-09-01 00:00:00
abstract::The function of a substantial percentage of the putative protein-coding open reading frames (ORFs) in viral genomes is unknown. As their sequence is not similar to that of proteins of known function, the function of these ORFs cannot be assigned on the basis of sequence similarity. Methods complement or in combination...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.10.020
更新日期:2005-01-05 00:00:00
abstract::The Autographa californica nuclear polyhedrosis virus da26 gene codes for an envelope protein of both budded virus (BV) and occlusion derived virus (ODV). Western blot and temporal analysis of infected cell extracts detected a protein of 26 kDa by 4 h postinfection (p.i.). The amount of protein increased by 16 h p.i. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8903
更新日期:1998-01-05 00:00:00
abstract::A range of deletion and other mutants in the coding region of the E1A gene of Ad5 has been assayed for transformation of baby rat kidney (BRK) cells in cooperation with ras, repression of the SV40 enhancer, and induction of proliferating cell nuclear antigen (PCNA). Transformation efficiency was drastically reduced by...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90518-7
更新日期:1989-07-01 00:00:00
abstract::Functions of the coat protein of white clover mosaic potexvirus (WCIMV) were investigated using C-terminal deletion mutants. Whereas plants inoculated with RNA transcripts of a full-length wild-type clone of WCIMV produced typical infections, plants inoculated with transcripts of each mutant did not produce symptoms, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90215-b
更新日期:1992-11-01 00:00:00
abstract::The tegument of herpes simplex virus contains proteins important for the efficient initiation of infection. L-particles are noninfectious virion-related particles that lack the nucleocapsid but do contain tegument and envelope. Their ability to deliver functional proteins into cells was compared to that of virions by ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90906-6
更新日期:1992-10-01 00:00:00
abstract::We demonstrate that the AL2 gene of Cabbage leaf curl virus (CaLCuV) activates the CP promoter in mesophyll and acts to derepress the promoter in vascular tissue, similar to that observed for Tomato golden mosaic virus (TGMV). Binding studies indicate that sequences mediating repression and activation of the TGMV and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.012
更新日期:2008-06-20 00:00:00
abstract::Hepatitis delta virus (HDV) encodes two proteins, the small hepatitis delta antigen (SHDAg) and large hepatitis delta antigen (LHDAg). Both proteins are identical except for the presence of additional 19 amino acids at the C terminus of LHDAg. While SHDAg is required for HDV RNA replication, LHDAg inhibits replication...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1109
更新日期:1994-02-15 00:00:00
abstract::The CD4+ T-cell-restricted recognition of the structural proteins in the JHM strain of MHV (JHMV) was compared between two mouse strains. Following immunization with inactivated JHMV, all proteins elicited an immune response in both C57BL/6 and BALB/c mouse strains, except the nucleocapsid (N) protein, which was not i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0608
更新日期:1996-11-15 00:00:00
abstract::Like human adenovirus type 5 (HAV5), bovine adenovirus type 3 (BAV3) early region 1 (E1) consists of E1A and E1B transcriptional units. In order to characterize BAV3 E1 proteins and to isolate a cell line of bovine origin that expresses BAV3 E1, polyclonal antibodies specific to E1A, E1B-157R, and E1B-420R were raised...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1389
更新日期:2002-03-30 00:00:00