Abstract:
:Pseudorabies virus (PRV) China reference strain Ea is genetically closely related to newly emerged variants; however, there is limited information about PRV Ea. Here, we compared PRV Ea with new variant strains by growth kinetics, genome sequencing, and protein expression analysis. Growth analysis showed that strain Ea forms smaller plaques than strain HNX. The full-length genome sequence of Ea revealed that it is clustered in the same subgroup as HNX. Ea and HNX strains exhibited similar extracellular virion protein polymorphisms, whereas strain Bartha expressed less VP26 and more GAPDH. In infected cells, strain Ea expressed high levels of IE180 protein, and Ea and HNX produced higher levels of UL21 protein than strain Bartha. These findings provide evidence that PRV China reference strain Ea is genetically closely related to the newly emerged variant strains, indicating that strain PRV China may have evolved independently leading to the emergence of a variant strain.
journal_name
Virologyjournal_title
Virologyauthors
Wang X,Wu CX,Song XR,Chen HC,Liu ZFdoi
10.1016/j.virol.2017.03.013subject
Has Abstractpub_date
2017-06-01 00:00:00pages
92-98eissn
0042-6822issn
1096-0341pii
S0042-6822(17)30095-8journal_volume
506pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Type III integral membrane proteins are oriented in the membrane with their C-terminus in the cytoplasm and their N-terminus extracytoplasmic. Such proteins are believed to have an internal hydrophobic sequence that functions both as an uncleaved signal for membrane insertion and also to anchor the protein in the memb...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1266
更新日期:1994-05-15 00:00:00
abstract::Electron microscopy and sequence analysis have suggested the presence of distinct morphological regions within the reovirus cell attachment protein sigma 1. Kinking of purified sigma 1 observed by electron microscopy and sequence-predicted flexibility profiles suggest the presence of potential flexible regions in the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1465
更新日期:1994-08-15 00:00:00
abstract::The M128L myxoma virus gene expresses a five-membrane spanning cell surface protein with significant amino acid homology to the cellular CD47 proteins. CD47, also called integrin-associated protein (IAP), is associated with the modulation of leukocyte adhesion, motility, activation, and phagocytosis. Creation of an M1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.03.037
更新日期:2005-06-20 00:00:00
abstract::Human rotaviruses (Wa, KUN, MO) showed hemagglutination (HA) only with fixed 1-day-old chicken erythrocytes, and their HA activities were completely destroyed by trypsin activation of virions. Simian SA-11 and bovine NCDV had HA activities not only against fixed erythrocytes but also against fresh erythrocytes from va...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90121-9
更新日期:1989-09-01 00:00:00
abstract::In this study, we investigated the autoimmune response in rabbit ovaries following infection with a recombinant myxoma virus expressing rabbit zona pellucida protein B (MV-ZPB). A specific IgG antibody response to ZPB was elicited in the serum of infected rabbits and the antibody strongly bound to the zona pellucida o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.10.021
更新日期:2004-01-20 00:00:00
abstract::Zika virus (ZIKV) nonstructural protein 5 (NS5) is a multifunctional protein possessing methyltransferase and RNA-dependent RNA polymerase activities. In the present study, we have carried out an extensive mutagenesis analysis to determine the importance of nuclear localization sequences (NLS) of NS5 in its nuclear ac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.10.010
更新日期:2020-02-01 00:00:00
abstract::The capsid protein sequences of 10 representative parvoviruses were aligned against the sequence and three-dimensional structure of canine parvovirus (CPV). The structure of CPV was then analyzed after mapping onto it position-dependent sequence similarity scores and the locations of residues that are phenotypically i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1288
更新日期:1993-06-01 00:00:00
abstract::By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and pa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)91205-9
更新日期:1992-09-01 00:00:00
abstract::Posttranslational modification of viral proteins by cellular enzymes is a feature of many virus replication strategies. Here, we report that during infection the multifunctional human influenza A virus NS1 protein is phosphorylated at threonine-215. Substitution of alanine for threonine at this position reduced early ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.10.002
更新日期:2009-01-05 00:00:00
abstract::Infection with bovine papillomavirus type 1 (BPV1) or BPV2 induces fibropapillomas in cows and skin sarcoids in horses. Prophylactic vaccination targeting BPV1 and BPV2 may reduce the incidence of these economically important diseases. The L1 major capsid proteins of BPV1 and BPV2 were expressed in Sf-9 insect cells a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.036
更新日期:2009-10-10 00:00:00
abstract::The DNAs of the Cas-Br-E MuLV-induced leukemias always contain somatically acquired mink cell focus-forming (MCF) recombinant proviruses. MCF recombinants could be involved during leukemogenesis at both preleukemic times and in late-stage tumors. Among the Cas-Br-E-induced non-T-, non-B-cell leukemias, viral integrati...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90241-g
更新日期:1992-12-01 00:00:00
abstract::HIV-1 genomic RNA (gRNA) dimerization is important for viral infectivity and is regulated by proteolytic processing of the Gag precursor protein (Pr55gag) under the direction of the viral protease. The processing occurs in successive steps and, to date, the step associated with formation of a wild-type (WT) level of g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.11.028
更新日期:2009-03-01 00:00:00
abstract::To better understand the role of specific residues within the duck hepatitis B virus (DHBV) pre-S protein in neutralization and infectivity, we have selected and identified pre-S variants which escape neutralization. A highly neutralizing monoclonal antibody (Mab 900) which recognizes an epitope 83IPQPQWTP90 localized...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8665
更新日期:1997-08-04 00:00:00
abstract::RSV is a major cause of severe lower respiratory infection in infants and young children. With no vaccine yet available, it is important to clarify mechanisms of disease pathogenesis. Since indoleamine-2,3-dioxygenase (IDO) is an immunomodulatory enzyme and is upregulated with RSV infection, we studied it in vivo duri...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.09.007
更新日期:2017-12-01 00:00:00
abstract::Equine influenza is caused by two serotypes of type A influenza virus, EIV-A1 and EIV-A2. The complete nucleotide sequence of the neuraminidase (NA) genes of both the A1 (N7 subtype) and A2 (N8 subtype) serotype has been determined following cloning of full-length viral NA cDNAs into pBR322. Analysis of the deduced am...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90207-2
更新日期:1986-12-01 00:00:00
abstract::The 5'-terminal noncoding region sequences were determined for the genome RNAs of seven strains of St. Louis encephalitis virus (SLEV) and one strain of West Nile virus (WNV) using a single synthetic cDNA primer complementary to the 5'-terminus of the coding region of a strain of WNV RNA. The 5'-terminal sequences obt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90468-0
更新日期:1988-02-01 00:00:00
abstract::In order to study the effect of nef gene expression on viral replication in monocytic cells, we established monocytic (U937 and THP-1) cell transfectants constitutively expressing the human immunodeficiency virus type 1 nef gene. We constructed a plasmid expressing the nef gene derived from an infectious clone, NL432,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90272-q
更新日期:1992-12-01 00:00:00
abstract::Deletion of the nef gene results in viral attenuation and confers protection against challenge with wild-type simian immunodeficiency virus in macaques. Regarding HIV-1 infection, a few long-term nonprogressors (LTNP) with nef deletions have been described. In this study, the nef genes of a group of seven LTNP and eig...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9783
更新日期:1999-07-05 00:00:00
abstract::The DNA sequence of the gag and pol regions of a provirus cloned from a bovine tumor is presented. In order to confirm these results the sequence of portions of a second clone, derived from a virus-producing cell line, was also determined. The gag gene was found to consist of 1179 nucleotides, which probably encode on...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90344-7
更新日期:1985-04-30 00:00:00
abstract::nsP3 is one of four viral nonstructural proteins required for RNA replication of Sindbis virus. In this report, post-translational modifications of nsP3 which occur in both vertebrate and mosquito cell cultures have been examined. In pulse-chase experiments, analyzed by immunoprecipitation and sodium dodecyl sulfate-p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90310-n
更新日期:1990-11-01 00:00:00
abstract::Turnip crinkle carmovirus (TCV) has a uniquely high recombination frequency and nonrandom cross-over site distribution among the recombining TCV-associated satellite RNAs. An in vitro system has been developed that includes a partially purified TCV replicase preparation (RdRp) and chimeric RNAs that resemble the putat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9342
更新日期:1998-09-30 00:00:00
abstract::Why some viruses establish chronic infections while others do not is poorly understood. One possibility is that the host's immune response is impaired during chronic infections and is unable to clear the virus from the host. In this report, we use a recently proposed framework to estimate the per capita killing effica...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.05.029
更新日期:2010-09-15 00:00:00
abstract::Retroviral Gag proteins function during early and late stages of the viral life cycle. To gain additional insight into the cellular requirements for viral replication, a two-hybrid screen was used to identify cellular proteins that interact with the Mason-Pfizer monkey virus Gag protein. One of the cellular proteins f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00348-9
更新日期:2003-09-15 00:00:00
abstract::The 20,349-bp sequence of the human cytomegalovirus (HCMV) HindIII F fragment has revealed eight open reading frames with homology to herpes simplex virus (HSV) and/or Epstein-Barr virus (EBV). With respect to EBV, these homologous genes can be divided into two blocks: one block contains three genes, including the DNA...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90282-0
更新日期:1987-04-01 00:00:00
abstract::Groundnut bud necrosis virus induces necrotic symptoms in different hosts. Previous studies showed reactive oxygen species-mediated programmed cell death (PCD) resulted in necrotic symptoms. Transgenic expression of viral protein NSs mimics viral symptoms. Here, we showed a role for NSs in influencing oxidative burst ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.05.003
更新日期:2017-08-01 00:00:00
abstract::Cellular activation plays an important role in retroviral replication. Previously, we have shown that CD4(+)CD25(+) T cells by the virtue of their partially activated phenotype represent ideal candidates for a productive feline immunodeficiency virus (FIV) infection. In the present study, we extended our previous obse...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.02.016
更新日期:2005-05-10 00:00:00
abstract::Commelina yellow mottle virus (CoYMV) is the type member of the badnaviruses, a genus of plant pararetroviruses. The N-terminus of the polyprotein encoded by ORF III has limited similarity to known cell-to-cell movement proteins. To test the hypothesis that the N-terminus is required for viral movement, the phenotypes...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8569
更新日期:1997-06-09 00:00:00
abstract::Several VV structural proteins are produced by the removal of amino-terminal peptides from their cognate precursors. In the experiments reported here, directed genetic approaches were used to investigate the possible role of these terminal peptides in protein processing. As a model system, the FLAG epitope-tagged P25K...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1069
更新日期:1995-02-20 00:00:00
abstract::Two sets of primers derived from the 5'-terminal region and the NS5 region of the hepatitis G virus (HGV) genome were used to amplify PCR fragments from serum specimens obtained from different parts of the world. All PCR fragments from the 5'-terminal region (5'-PCR, n = 56) and from the NS5 region (NS5-PCR, n = 85) w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9592
更新日期:1999-03-15 00:00:00
abstract::The glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV) serves as virus attachment protein to its receptor on host cells and is a key determinant for cell tropism, pathogenesis, and epidemiology of the virus. The GP of LCMV is posttranslationally cleaved by the subtilase SKI-1/S1P into two subunits, the per...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00421-5
更新日期:2003-09-15 00:00:00