Sulfatide inhibits HIV-1 entry into CD4-/CXCR4+ cells.


:Sulfatide (3'sulfogalactosylceramide) is the natural sulfated derivative of galactosylceramide (GalCer), a glycosphingolipid receptor allowing HIV-1 infection of CD4-negative cells from neural and intestinal tissues. The incorporation of exogenous sulfatide into the plasma membrane of HT-29 (a CD4-/GalCer+/CXCR4+ human intestinal cell line) or RD (CD4-/GalCer-/ CXCR4+ human rhabdomyosarcoma) resulted in a dose-dependent inhibition of HIV-1 infection. Experiments with luciferase reporter viruses pseudotyped with HIV-1 or amphotropic murine leukemia virus envelopes demonstrated that sulfatide acts at the level of viral entry. Paradoxically, the transfer of sulfatide in the plasma membrane of various CD4- cells resulted in increased binding of HIV-1. Surface pressure measurements were conducted to study the interaction of gp120 with glycosphingolipid monolayers. The data showed that gp120 could penetrate into a monomolecular film of GalCer, confirming the role of this glycosphingolipid as a functional receptor for HIV-1. In contrast, the insertion of gp120 into a monolayer of sulfatide was very limited. Moreover, the incorporation of sulfatide in a monomolecular film of GalCer specifically inhibited the penetration of gp120. In conclusion, these data show that sulfatide mediates gp120 binding but, in marked contrast with GalCer, is not able to initiate the fusion event.






Fantini J,Hammache D,Delézay O,Piéroni G,Tamalet C,Yahi N




Has Abstract


1998-07-05 00:00:00
















  • Detection of visna virus antigens and RNA in glial cells in foci of demyelination.

    abstract::Visna is a slow virus infection of sheep in which the characteristic pathological change is demyelination in foci of inflammation. The latter is thought to be the result of an immunopathological process directed against cellular and antigenic targets that have been difficult to define because of restricted viral gene ...


    pub_type: 杂志文章


    authors: Stowring L,Haase AT,Petursson G,Georgsson G,Palsson P,Lutley R,Roos R,Szuchet S

    更新日期:1985-03-01 00:00:00

  • Substantial envelope-specific CD8 T-cell immunity fails to control SIV disease.

    abstract::It is unknown which HIV proteins to target by vaccination in order to generate the most effective CD8 T-cell immunity. We recently immunized SIV(mac251)-infected pigtail macaques with Gag peptides or a cocktail of peptides spanning all SIV proteins, including SIV Env. High-level SIV Env-specific CD8 T-cell responses w...


    pub_type: 杂志文章


    authors: Peut V,Kent SJ

    更新日期:2009-02-05 00:00:00

  • Deletion mutants of region E1a of AD12 E1 plasmids: effect on oncogenic transformation.

    abstract::Plasmids containing the E1 region of Ad12 DNA can transform certain rodent cells into oncogenic cells. To study the role of the E1a subregion in the process of oncogenic transformation, Ad12 region E1 mutants carrying deletions in the E1a region were constructed. Deletion mutants pR7 and pR8 affect only the 13 S mRNA ...


    pub_type: 杂志文章


    authors: Bos JL,Jochemsen AG,Bernards R,Schrier PI,van Ormondt H,van der Eb AJ

    更新日期:1983-09-01 00:00:00

  • Q-mediated late gene transcription of bacteriophage lambda: RNA start point and RNase III processing sites in vivo.

    abstract::The location of the RNA start point of in vivo Q-activated late gene RNA of bacteriophage lambda has been determined to be identical to the start point of in vitro 6 S RNA. The 6 S RNA is made early in infection and is efficiently antiterminated by Q. Two RNase III cut sites are located within Q-dependent RNA sequence...


    pub_type: 杂志文章


    authors: Daniels DL,Subbarao MN,Blattner FR,Lozeron HA

    更新日期:1988-12-01 00:00:00

  • Inhibition of vaccinia virus replication by N-(phosphonoacetyl)-L-aspartate: differential effects on viral gene expression result from a reduced pyrimidine nucleotide pool.

    abstract::The replication of vaccinia virus was reduced by 3 logs in cells that had been treated before and during infection with a concentration of N-(phosphonoacetyl)-L-aspartate (PALA) which lowered the UTP and CTP to 5 and 20% of controls, respectively, without affecting cell viability. The antiviral activity of PALA was re...


    pub_type: 杂志文章


    authors: Katsafanas GC,Grem JL,Blough HA,Moss B

    更新日期:1997-09-15 00:00:00

  • Reovirus exists in the form of 13 particle species that differ in their content of protein sigma 1.

    abstract::When electrophoresed in 0.7% agarose gels, populations of reovirus particles can be resolved into 13 well-defined bands that possess from 0 to 12 projection/spike-associated trimers of protein sigma 1. This state of affairs is not an artifact of purification, of the techniques used to demonstrate it, of aggregation, o...


    pub_type: 杂志文章


    authors: Larson SM,Antczak JB,Joklik WK

    更新日期:1994-06-01 00:00:00

  • Minimum requirements for specific binding of RNA and coat protein of alfalfa mosaic virus.

    abstract::Coat protein-protected fragments of alfalfa mosaic virus RNA (AlMV-RNA) and tobacco streak virus RNA (TSV-RNA), which were isolated as described [D. Zuidema, M. F. A. Bierhuizen, B. J. C. Cornelissen, J. F. Bol, and E. M. J. Jaspars (1983)Virology, 125, 361-369], were tested for their ability to rebind AlMV coat prote...


    pub_type: 杂志文章


    authors: Zuidema D,Cool RH,Jaspars EM

    更新日期:1984-07-30 00:00:00

  • Antigenic variation of SIV: mutations in V4 alter the neutralization profile.

    abstract::Antigenic variation is a characteristic feature of lentiviral infection. The SIV/macaque model of AIDS provides an ideal system in which to investigate the molecular basis of antigenic variation. The purpose of this study was to genetically map the nucleotide changes in env that alter the neutralization phenotype of S...


    pub_type: 杂志文章


    authors: Kinsey NE,Anderson MG,Unangst TJ,Joag SV,Narayan O,Zink MC,Clements JE

    更新日期:1996-07-01 00:00:00

  • The complete cDNA sequence of a type II Trichomonas vaginalis virus.

    abstract::Trichomonas vaginalis viruses (TVV), which may regulate P270 gene expression in the protozoan pathogen T. vaginalis, are a group of divergent double-stranded (ds) RNA viruses. In the present study, the complete 4674-bp cDNA sequence of a 4.6-kb ds RNA from a newly identified TVV2-1 isolate was determined. The sequence...


    pub_type: 杂志文章


    authors: Bessarab IN,Liu HW,Ip CF,Tai JH

    更新日期:2000-02-15 00:00:00

  • Immune responses but no protection against SHIV by gene-gun delivery of HIV-1 DNA followed by recombinant subunit protein boosts.

    abstract::The efficacy of combining immunization with human immunodeficiency vitus type 1 (HIV-1) DNA and HIV-1 recombinant proteins to obtain protection from chimeric simian/human immunodeficiency virus (SHIV) was determined. Four cynomolgus monkeys received four gene-gun immunizations intraepidermally of plasmid DNA encoding ...


    pub_type: 杂志文章


    authors: Putkonen P,Quesada-Rolander M,Leandersson AC,Schwartz S,Thorstensson R,Okuda K,Wahren B,Hinkula J

    更新日期:1998-10-25 00:00:00

  • The flavivirus-conserved penta-nucleotide in the 3' stem-loop of the West Nile virus genome requires a specific sequence and structure for RNA synthesis, but not for viral translation.

    abstract::A reporting replicon of West Nile virus (WN) was used to distinguish between the function of the 3' untranslated region (UTR) in viral translation and RNA replication. Deletions of various regions of the 3' UTR of the replicon did not significantly affect viral translation, but abolished RNA replication. A systematic ...


    pub_type: 杂志文章


    authors: Tilgner M,Deas TS,Shi PY

    更新日期:2005-01-20 00:00:00

  • Immune effector mechanisms required for protection to rabies virus.

    abstract::Genetically engineered mice with targeted mutations in genes encoding immunologically relevant molecules were used to elucidate the role of different immune effector mechanisms in protection against a rabies virus (RV) infection. In vaccinated animals challenged with a highly virulent strain of RV, antibodies were cru...


    pub_type: 杂志文章


    authors: Xiang ZQ,Knowles BB,McCarrick JW,Ertl HC

    更新日期:1995-12-20 00:00:00

  • Octapartite negative-sense RNA genome of High Plains wheat mosaic virus encodes two suppressors of RNA silencing.

    abstract::High Plains wheat mosaic virus (HPWMoV, genus Emaravirus; family Fimoviridae), transmitted by the wheat curl mite (Aceria tosichella Keifer), harbors a monocistronic octapartite single-stranded negative-sense RNA genome. In this study, putative proteins encoded by HPWMoV genomic RNAs 2-8 were screened for potential RN...


    pub_type: 杂志文章


    authors: Gupta AK,Hein GL,Graybosch RA,Tatineni S

    更新日期:2018-05-01 00:00:00

  • Epigenetics of human papillomaviruses.

    abstract::Human papilllomaviruses (HPVs) are common human pathogens that infect cutaneous or mucosal epithelia in which they cause warts, self-contained benign lesions that commonly regress. The HPV life cycle is intricately tied to the differentiation of the host epithelium it infects. Mucosotropic HPVs are the most common sex...


    pub_type: 杂志文章


    authors: Johannsen E,Lambert PF

    更新日期:2013-10-01 00:00:00

  • Characterization of a novel alphaherpesvirus associated with fatal infections of domestic rabbits.

    abstract::A virus was found to be associated with a severe disease affecting rabbits on a farm near Anchorage, Alaska. Extracts from the skin of infected rabbits produced syncytia and cell lysis in cultured rabbit skin, rabbit kidney, and Vero cells. Examination of the infectious agent by electron microscopy revealed an icosahe...


    pub_type: 杂志文章


    authors: Jin L,Löhr CV,Vanarsdall AL,Baker RJ,Moerdyk-Schauwecker M,Levine C,Gerlach RF,Cohen SA,Alvarado DE,Rohrmann GF

    更新日期:2008-08-15 00:00:00

  • In vitro fidelity of the prototype primate foamy virus (PFV) RT compared to HIV-1 RT.

    abstract::We compared the in vitro fidelity of wild-type human immunodeficiency virus type-1 (HIV-1) reverse transcriptase (RT) and the prototype foamy virus (PFV) RT. Both enzymes had similar error rates for single nucleotide substitutions; however, PFV RT did not appear to make errors at specific hotspots, like HIV-1 RT. In a...


    pub_type: 杂志文章


    authors: Boyer PL,Stenbak CR,Hoberman D,Linial ML,Hughes SH

    更新日期:2007-10-25 00:00:00

  • Paramyxovirus Sendai virus-like particle formation by expression of multiple viral proteins and acceleration of its release by C protein.

    abstract::Envelope viruses maturate by macromolecule assembly and budding. To investigate these steps, we generated virus-like particles (VLPs) by co-expression of structural proteins of Sendai virus (SeV), a prototype of the family Paramyxoviridae. Simultaneous expression of matrix (M), nucleo- (N), fusion (F), and hemagglutin...


    pub_type: 杂志文章


    authors: Sugahara F,Uchiyama T,Watanabe H,Shimazu Y,Kuwayama M,Fujii Y,Kiyotani K,Adachi A,Kohno N,Yoshida T,Sakaguchi T

    更新日期:2004-07-20 00:00:00

  • Proteolytic processing of Marburg virus glycoprotein.

    abstract::Processing of the transmembrane glycoprotein (GP) of Marburg virus involved the conversion of an endo H-sensitive, ER-specific form into an endo H-resistant, Golgi-specific precursor that was cleaved into GP(1) and GP(2). Cleavage was mediated by furin or another subtilisin-like endoprotease with similar substrate spe...


    pub_type: 杂志文章


    authors: Volchkov VE,Volchkova VA,Ströher U,Becker S,Dolnik O,Cieplik M,Garten W,Klenk HD,Feldmann H

    更新日期:2000-03-01 00:00:00

  • Analysis of the retrovirus capsid interdomain linker region.

    abstract::In structural studies, the retrovirus capsid interdomain linker region has been shown as a flexible connector between the CA N-terminal domain and its C-terminal domain. To analyze the function of the linker region, we have examined the effects of three Moloney murine leukemia virus (M-MuLV) capsid linker mutations/va...


    pub_type: 杂志文章


    authors: Arvidson B,Seeds J,Webb M,Finlay L,Barklis E

    更新日期:2003-03-30 00:00:00

  • In Nicotiana species, an artificial microRNA corresponding to the virulence modulating region of Potato spindle tuber viroid directs RNA silencing of a soluble inorganic pyrophosphatase gene and the development of abnormal phenotypes.

    abstract::Potato spindle tuber viroid (PSTVd) is a small non-protein-coding RNA pathogen that can induce disease symptoms in a variety of plant species. How PSTVd induces disease symptoms is a long standing question. It has been suggested that PSTVd-derived small RNAs (sRNAs) could direct RNA silencing of a targeted host gene(s...


    pub_type: 杂志文章


    authors: Eamens AL,Smith NA,Dennis ES,Wassenegger M,Wang MB

    更新日期:2014-02-01 00:00:00

  • Discovery, diversity and evolution of novel coronaviruses sampled from rodents in China.

    abstract::Although rodents are important reservoirs for RNA viruses, to date only one species of rodent coronavirus (CoV) has been identified. Herein, we describe a new CoV, denoted Lucheng Rn rat coronavirus (LRNV), and novel variants of two Betacoronavirus species termed Longquan Aa mouse coronavirus (LAMV) and Longquan Rl ra...


    pub_type: 杂志文章


    authors: Wang W,Lin XD,Guo WP,Zhou RH,Wang MR,Wang CQ,Ge S,Mei SH,Li MH,Shi M,Holmes EC,Zhang YZ

    更新日期:2015-01-01 00:00:00

  • Early activation of mitogen-activated protein kinase kinase, extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and c-Jun N-terminal kinase in response to binding of simian immunodeficiency virus to Jurkat T cells expressing CCR5

    abstract::We have shown that the binding of simian immunodeficiency virus (SIV) to Jurkat T cells expressing CD4 receptor strongly induces mitogen-activated protein (MAP) kinase kinase (MEK) and extracellular signal-regulated kinases 1 and 2 (ERK1/2) and only weakly induces p38 MAP kinase and c-Jun N-terminal kinase (JNK). Simi...


    pub_type: 杂志文章


    authors: Popik W,Pitha PM

    更新日期:1998-12-05 00:00:00

  • The effect of ribavirin and IMPDH inhibitors on hepatitis C virus subgenomic replicon RNA.

    abstract::The recent development of in vitro hepatitis C virus (HCV) RNA replication systems has provided useful tools for studying the intracellular anti-HCV activity of ribavirin. Ribavirin has been shown to: (1) induce "error catastrophe" in poliovirus, Proc. Natl. Acad. Sci. USA 98, 6895-6900), (2) be a pseudo-substrate of ...


    pub_type: 杂志文章


    authors: Zhou S,Liu R,Baroudy BM,Malcolm BA,Reyes GR

    更新日期:2003-06-05 00:00:00

  • Membranes of herpes simplex virus type-1-infected human corneal epithelial cells are not permeabilized to macromolecules and therefore do not release IL-1alpha.

    abstract::Nanogram amounts of the proinflammatory cytokine interleukin-1alpha (IL-1alpha) were detected in uninfected cultures of human corneal epithelial cells (HCEC). Although HSV-1 replicated >10(4)-fold in these cells and caused extensive cytopathic effects, virus infection was not accompanied by significant extracellular r...


    pub_type: 杂志文章


    authors: Tran MT,Dean DA,Lausch RN,Oakes JE

    更新日期:1998-04-25 00:00:00

  • Pneumotropic revertants derived from a pantropic mutant, F1-R, of Sendai virus.

    abstract::Revertants were isolated from the protease activation mutant of Sendai virus, F1-R, which causes a systemic infection in mice. The fusion (F) glycoprotein of F1-R is susceptible to activation cleavage by ubiquitous cellular proteases and is thus responsible for pantropism in mice (Tashiro et al., 1988. Virology 165, 5...


    pub_type: 杂志文章


    authors: Tashiro M,James I,Karri S,Wahn K,Tobita K,Klenk HD,Rott R,Seto JT

    更新日期:1991-09-01 00:00:00

  • Vaccinia virus exhibits cell-type-dependent entry characteristics.

    abstract::Differing and sometimes conflicting data have been reported regarding several aspects of vaccinia virus (VV) entry. To address this, we used a beta-galactosidase reporter virus to monitor virus entry into multiple cell types under varying conditions. Entry into HeLa, B78H1 and L cells was strongly inhibited by heparin...


    pub_type: 杂志文章


    authors: Whitbeck JC,Foo CH,Ponce de Leon M,Eisenberg RJ,Cohen GH

    更新日期:2009-03-15 00:00:00

  • Nucleocytoplasmic shuttling of the human parainfluenza virus type 2 phosphoprotein.

    abstract::Human parainfluenza virus type 2 phosphoprotein (P) is an essential component of viral polymerase. The P gene encodes both P and accessory V proteins by a specific gene editing mechanism. Therefore, the N-terminal 164 amino acids of P protein are common to V protein. Interestingly, while P protein is located in the cy...


    pub_type: 杂志文章


    authors: Ohtsuka J,Matsumoto Y,Ohta K,Fukumura M,Tsurudome M,Nosaka T,Nishio M

    更新日期:2019-02-01 00:00:00

  • A reassortant between influenza A viruses (H7N2) synthesizing an enzymatically inactive neuraminidase at 40 degrees which is not incorporated into infectious particles.

    abstract::Cells infected with a reassortant (113/Ho, H7N2) between A/fowl plague/Rostock/34 (FPV, H7N1) and A/Hong Kong/1/68 (H3N2) carrying RNA segments 1 and 6 of the Hong Kong virus and the residual genes of FPV, synthesized at 40 degrees a neuraminidase (NA) which is enzymatically not active and which is not incorporated in...


    pub_type: 杂志文章


    authors: Breuning A,Scholtissek C

    更新日期:1986-04-15 00:00:00

  • Residues in the PB2 and PA genes contribute to the pathogenicity of avian H7N3 influenza A virus in DBA/2 mice.

    abstract::Replication and transmission of avian influenza virus in humans poses a pandemic threat. The molecular determinants that facilitate this process are not well understood. We used DBA/2 mice to identify viral factors that mediate the difference in pathogenesis between a virulent (H7N3) and a non-virulent (H7N9) avian in...


    pub_type: 杂志文章


    authors: DesRochers BL,Chen RE,Gounder AP,Pinto AK,Bricker T,Linton CN,Rogers CD,Williams GD,Webby RJ,Boon AC

    更新日期:2016-07-01 00:00:00

  • High levels of SIVmnd-1 replication in chronically infected Mandrillus sphinx.

    abstract::Viral loads were investigated in SIVmnd-1 chronically infected mandrills and the results were compared with those previously observed in other nonpathogenic natural SIV infections. Four naturally and 11 experimentally SIVmnd-1-infected mandrills from a semi-free-ranging colony were studied during the chronic phase of ...


    pub_type: 杂志文章


    authors: Pandrea I,Onanga R,Kornfeld C,Rouquet P,Bourry O,Clifford S,Telfer PT,Abernethy K,White LT,Ngari P,Müller-Trutwin M,Roques P,Marx PA,Simon F,Apetrei C

    更新日期:2003-12-05 00:00:00