Abstract:
:Cytoplasmic tails of envelope (Env) glycoproteins of many retroviruses inhibit their membrane fusion activity. The cytoplasmic 16-amino acid peptide of ecotropic murine leukemia virus (E-MLV) Env protein, called the R-peptide, also inhibits the membrane fusion activity of the Env protein. However, the molecular mechanism of the inhibition has not been elucidated yet. In this study, we found that R-peptide-containing Env protein of E-MLV binds to the cell surface receptor cationic amino acid transporter-1 (CAT-1) with weaker affinity than R-peptide-truncated Env protein. Consistent with this result, R-peptide-containing Env protein had less efficient inhibition of E-MLV vector infection than R-peptide-truncated Env protein. R-peptide truncation has been reported to induce conformational change in the surface subunit of E-MLV Env protein that interacts with the receptor. Taken together, our findings indicate that R-peptide truncation induces conformational change in the receptor-binding domain of the E-MLV Env protein and facilitates the Env-receptor interaction.
journal_name
Virologyjournal_title
Virologyauthors
Kubo Y,Izumida M,Togawa K,Zhang F,Hayashi Hdoi
10.1016/j.virol.2019.04.005subject
Has Abstractpub_date
2019-06-01 00:00:00pages
82-87eissn
0042-6822issn
1096-0341pii
S0042-6822(19)30105-9journal_volume
532pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Previously our laboratory constructed an HIV-1 which stably maintained a primer binding site (PBS) complementary to tRNA(His) by mutating the region of the provirus within U5 postulated to interact with the anticodon of tRNA(His) (J. Wakefield, S-M Kang, and C. D. Morrow, 1996, J. Virol, 70, 966-975). From the analysi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8837
更新日期:1997-11-24 00:00:00
abstract::Tomato strain L of tobacco mosaic virus (TMV-L) induces a hypersensitive response (necrotic local lesions) on tobacco plants with the N' gene. A factor responsible for induction of the hypersensitive response has been mapped to the coat protein gene. We have constructed several mutants which have insertions or deletio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90217-1
更新日期:1989-11-01 00:00:00
abstract::Geminiviruses are small, ssDNA-containing plant viruses. Bean golden mosaic virus (BGMV) and tomato golden mosaic virus (TGMV) have bipartite genomes, the components of which are designated A and B. Although they are closely related, BGMV and TGMV nevertheless exhibit distinct host-specific phenotypes, with BGMV being...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9424
更新日期:1998-11-25 00:00:00
abstract::The Sindbis virus mutant SB-RL, in contrast to its parent, Sindbis strain AR339 (SB), is attenuated in neonatal mice, has an increased rate of penetration in tissue culture cells, and is more sensitive to neutralization by E2-specific monoclonal antibodies (MCAbs) R6 and R13. These phenotypic differences are controlle...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90184-s
更新日期:1990-03-01 00:00:00
abstract::The enveloped virions of two strains of a granulosis virus, a synergistic Hawaiian (GVH) and a nonsynergistic Oregonian (GVO) strain which infect the armyworm, Pseudaletia unipuncta, were liberated from their capsules with 0.02 N NaOH and purified by filtration through membrane filters. The envelopes were solubilized ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90439-2
更新日期:1979-04-15 00:00:00
abstract::The reactivates of human sera with uninduced and phorbol ester (TPA)-induced human herpesvirus-8 (HHV-8)-infected BCBL-1 cells were examined by immunofluorescence assay (IFA) and by radioimmunoprecipitation reactions (RIP). The seroprevalence of HHV-8 infections is low in the United States general population and only ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9055
更新日期:1998-03-30 00:00:00
abstract::Viral metagenomics coupled to high-throughput sequencing has provided a powerful tool for large-scale detection of known and unknown viruses associated to distinct hosts and environments. Using this approach, known and novel viruses have been characterized from sylvatic and commercial avian hosts, increasing our under...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.05.013
更新日期:2020-09-01 00:00:00
abstract::Neutralization epitopes of duck hepatitis B virus (DHBV) have been previously mapped within the N-terminal portion of the pre-S protein using monoclonal antibodies. However, the immune response of ducks to this region is not well characterized at the amino acid level. To this end, we have immunized adult Pekin ducks w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1164
更新日期:1994-04-01 00:00:00
abstract::It has previously been shown that a far-upstream region of the herpes simplex virus immediate early (IE) gene 3 increases the expression of a linked thymidine kinase (TK) gene and also contains sequences which respond to stimulation of transcription by a virion component. The effects of altering the orientation and di...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90238-1
更新日期:1984-09-01 00:00:00
abstract::Fowlpox virus (FPV), type species of the Avipoxvirus genus, causes a slow-spreading pox disease of chickens. Following infection of mammalian cells there is no evidence of productive replication of FPV although cytopathic effects are induced and FPV recombinants have been shown to express foreign genes from vaccinia v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1608
更新日期:1993-11-01 00:00:00
abstract::Transcriptionally targeted MLV-based ProCon vectors allow expression of the transduced gene in a promoter-specific manner by replacement of the viral U3 region with a heterologous promoter. In order to evaluate the effects of sequence elements present in ProCon vectors on transgene expression (enhanced green fluoresce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.09.012
更新日期:2004-12-05 00:00:00
abstract::Human papillomavirus type 16 E7 protein, expressed in Escherichia coli as fusion protein lac alpha peptide-E7 (lac-E7), was purified by electrophoresis and used to immunize rabbits to raise antisera. The anti-lac-E7 sera recognized another bacterially expressed fusion protein trpE-E7 by the Western blot method. The an...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90386-3
更新日期:1989-05-01 00:00:00
abstract::Insertion of a functional splicing cassette into a construct containing the HIV-1 poly(A) site followed by the adenovirus L3 poly(A) site results in both specific stimulation of 3' end processing at the HIV-1 site and an increase in the steady-state levels of RNA processed at both sites. To further evaluate this influ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0556
更新日期:1996-10-15 00:00:00
abstract::Infection of rhesus macaques with attenuated SIVmac is, at present, the only strategy which confers significant protection from challenge with wild-type SIVmac grown in monkey PBMC. However, initial results suggest that the protective mechanism does not develop until late after "vaccination" (approx 10 months). As par...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0237
更新日期:1996-05-01 00:00:00
abstract::Some retroviruses, including HIV-1, regulate the relative amounts of gag and pol gene products by a translational frameshift mechanism. The consequences of altering the ratios of the Gag and Pol proteins were tested using vaccinia virus expression vectors, in which the gag and pol genes were fused by placing them in t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1174
更新日期:1993-04-01 00:00:00
abstract::Hepatitis C virus (HCV) was shown to activate protein kinase R (PKR), which inhibits expression of interferon (IFN) and IFN-stimulated genes by controlling the translation of newly transcribed mRNAs. However, it is unknown exactly how HCV activates PKR. To address the molecular mechanism(s) of PKR activation mediated ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.01.024
更新日期:2019-03-01 00:00:00
abstract::During replication, the lengthy genome of dsDNA viruses is translocated with remarkable velocity into the limited space within the preformed procapsid. We previously found that a viral-encoded RNA (pRNA) played a key role in bacterial virus phi29 DNA translocation. Design of mutant pRNA sets containing two and three i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0771
更新日期:2001-03-15 00:00:00
abstract::Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) is an orphan nuclear receptor that represses transcription of many genes. In adenovirus type 12 (Ad12) transformed cells, a high level of binding activity of COUP-TF to the major histocompatibility complex (MHC) class I enhancer correlates with the dow...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0181
更新日期:2000-03-15 00:00:00
abstract::Epstein-Barr virus (EBV) is known to infect B cells and epithelial cells. We and others have shown that EBV can also infect a subset of thymocytes. Infection of thymocytes was accompanied by the appearance of linear EBV genome within 8 hr of infection. Circularization of the EBV genome was not detected. This is in con...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1200
更新日期:1995-04-20 00:00:00
abstract::To be incorporated into progeny virions, the viral genome must be transported to the inner leaflet of the plasma membrane (PM) and accumulate there. Some viruses utilize lipid components to assemble at the PM. For example, simian virus 40 (SV40) targets the ganglioside GM1 and human immunodeficiency virus type 1 (HIV-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.05.018
更新日期:2016-09-01 00:00:00
abstract::Red clover necrotic mosaic virus (RCNMV) encodes N-terminally overlapping proteins of 27 and 88 kDa (p27 and p88) known to be required for replication. Green fluorescent protein (GFP) fusions were used to visualize the location of p27 and p88 within Nicotiana benthamiana cells. GFP:p27 fusions localized to the endopla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.12.006
更新日期:2004-03-15 00:00:00
abstract::A viroid has been purified from avocado leaves infected by sunblotch disease and designated the avocado sunblotch viroid. It is a covalently closed circular RNA molecule with a molecular weight lower than that of chrysanthemum stunt viroid and citrus exocortis viroid while hybridization analysis with 32P-labeled compl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90045-x
更新日期:1979-11-01 00:00:00
abstract::Human immunodeficiency virus (HIV) encodes a transacting protein, Rev, which interacts with an RNA element (RRE) to mediate nuclear export of unspliced viral mRNA. Recently, the RNA constitutive transport elements (CTE) from Mason-Pfizer monkey virus (MPMV) and simian retrovirus type I (SRV-1) were shown to render Rev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8407
更新日期:1997-02-17 00:00:00
abstract::West Nile virus (WNV) genome cyclization is mediated by two pairs of long-distance RNA/RNA interactions: the 5'CS/3'CSI (conserved sequence) and the 5'UAR/3'UAR (upstream AUG region) base pairings. Antisense peptide-conjugated phosphorodiamidate morpholino oligomers (PPMOs), designed to interfere with the 5'CS/3'CSI o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.044
更新日期:2008-12-05 00:00:00
abstract::The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two single-stranded RNA species termed RNA-1 and RNA-2. RNA-2 is required for infection of whole plants but is dispensable for infection and virion formation in protoplasts. We have used full-length cDNA clones of RNA-1 and -2 from which infec...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1004
更新日期:1993-01-01 00:00:00
abstract::Mouse mammary tumor virus (MMTV) is a pH-dependent virus that uses mouse transferrin receptor 1 (TfR1) for entry into cells. Previous studies demonstrated that MMTV could induce pH 5-dependent fusion-from-with of mouse cells. Here we show that the MMTV envelope-mediated cell-cell fusion requires both the entry recepto...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.013
更新日期:2008-11-25 00:00:00
abstract::Bovine respiratory disease (BRD) is the costliest disease affecting the cattle industry globally. Orthomyxoviruses, influenza C virus (ICV) and influenza D virus (IDV) have recently been implicated to play a role in BRD. However, there are contradicting reports about the association of IDV and ICV to BRD. Using the la...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.08.014
更新日期:2020-12-01 00:00:00
abstract::Phage-display methodology has been used to select variant CD4 proteins exhibiting increased binding to the surface envelope glycoprotein, gp120, of Human Immunodeficiency Virus Type-1. To facilitate the selection, a library of mutant CD4 proteins was constructed by cloning a PCR-generated error prone population of the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8651
更新日期:1997-08-04 00:00:00
abstract::Baculoviruses encode fibroblast growth factor (vfgf) homologs whose function during virus infection is unknown. We constructed a recombinant bacmid of Autographa californica M nucleopolyhedrovirus (AcMNPV) lacking a functional vfgf and characterized it in two insect cell lines. The kinetics of budded virus production ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.01.016
更新日期:2006-03-15 00:00:00
abstract::Mammalian type C retroviruses can be detected by their induction of foci of cell transformation in S+I - cells. We have noted that certain subtypes of the mouse xenotropic type C retrovirus (MuLV) inhibit this cell alteration. This inhibition, associated with intact virions, is irreversible and gives the infected cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90044-4
更新日期:1987-09-01 00:00:00