Abstract:
:Hepatitis C virus (HCV) was shown to activate protein kinase R (PKR), which inhibits expression of interferon (IFN) and IFN-stimulated genes by controlling the translation of newly transcribed mRNAs. However, it is unknown exactly how HCV activates PKR. To address the molecular mechanism(s) of PKR activation mediated by HCV infection, we examined the effects of viral proteins on PKR activation. Here, we show that expression of HCV NS5B strongly induced PKR and eIF2α phosphorylation, and attenuated MHC class I expression. In contrast, expression of Japanese encephalitis virus RNA-dependent RNA polymerase did not induce phosphorylation of PKR. Co-immunoprecipitation analyses showed that HCV NS5B interacted with PKR. Furthermore, expression of NS5B with polymerase activity-deficient mutation failed to phosphorylate PKR, suggesting that RNA polymerase activity is required for PKR activation. These results suggest that HCV activates PKR by association with NS5B, resulting in translational suppression of MHC class I to establish chronic infection.
journal_name
Virologyjournal_title
Virologyauthors
Suzuki R,Matsuda M,Shimoike T,Watashi K,Aizaki H,Kato T,Suzuki T,Muramatsu M,Wakita Tdoi
10.1016/j.virol.2019.01.024subject
Has Abstractpub_date
2019-03-01 00:00:00pages
226-233eissn
0042-6822issn
1096-0341pii
S0042-6822(19)30028-5journal_volume
529pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Nucleotide sequence analysis of the genome of the baculovirus Bombyx mori nuclear polyhedrosis virus (BmNPV) identified 18 homologues of the Autographa californica NPV (AcNPV) lefs (late expression factor genes). These BmNPV lefs showed high (73-98%) amino acid sequence identities to AcNPV lefs and were localized to s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8457
更新日期:1997-03-31 00:00:00
abstract::HIV-1 virus entry into target cells requires the envelope glycoprotein (Env) to first bind the primary receptor, CD4 and subsequently the co-receptor. Antibody access to the co-receptor binding site (CoRbs) in the pre-receptor-engaged state, prior to cell attachment, remains poorly understood. Here, we have demonstrat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.04.002
更新日期:2018-06-01 00:00:00
abstract::Rice tungro bacilliform virus (RTBV) is a plant pararetrovirus and a member of the Caulimoviridae family and closely related to viruses in the Badnavirus genus. The coat protein of RTBV is part of the large polyprotein encoded by open reading frame 3 (ORF3). ORF3 of an RTBV isolate from Malaysia was sequenced (accessi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9519
更新日期:1999-01-20 00:00:00
abstract::Multibranched peptides (SPCs) derived either from the fusion protein (gp41) sequence or from the cleavage sequence of the human immunodeficiency virus type 1 envelope were chemically synthesized and tested for their ability to inhibit both syncytium formation and HIV production in CD4+ cells. The gp41-derived SPCs had...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0496
更新日期:1996-09-15 00:00:00
abstract::Infection with the Tucson strain of coxsackievirus B1 (CVB1T) causes the development of chronic inflammatory myopathy (CIM) and hind limb weakness in susceptible strains of mice. In this study, a panel of six plaque-purified viruses exhibiting either small or large plaque phenotypes was derived from parental CVB1T and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8592
更新日期:1997-06-23 00:00:00
abstract::The genome of the Bacillus subtilis phage SP01 contains a 12.6-kilobase-pair terminal redundancy. The transcription units in this region, which are utilized in vitro by B. subtilis RNA polymerase holoenzme, have been mapped. In vitro transcripts were separated and characterized by gel electrophoresis. Since there is s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90361-5
更新日期:1981-06-01 00:00:00
abstract::In order to molecularly characterize rapidly and slowly replicating HIV-1 variants, molecular clones were obtained from a rapid/high virus isolate. This isolate, 4803, had only been passaged in peripheral blood mononuclear cells (PBMC) prior to cloning. Molecular cloning was done in bacteriophage lambda-dash using hig...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90469-r
更新日期:1991-03-01 00:00:00
abstract::We used serial protein misfolding cyclic amplification (sPMCA) to amplify the D10 strain of CWD prions in a linear relationship over two logs of D10 dilutions. The resultant PMCA-amplified D10 induced terminal TSE disease in CWD-susceptible Tg(cerPrP)1536 mice with a survival time approximately 80 days shorter than th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.09.023
更新日期:2008-12-20 00:00:00
abstract::The human APOBEC3G protein exhibits broad antiretroviral activity against a variety of retroviruses. It is packaged into viral particles and executes its antiviral function in the target cell. The packaging of APOBEC3G into different viral particles requires a mechanism that confers this promiscuity. Here, APOBEC3G in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.04.006
更新日期:2005-06-20 00:00:00
abstract::Using the complete genome sequences of 19 coronavirus genomes, we analyzed the codon usage bias, dinucleotide relative abundance and cytosine deamination in coronavirus genomes. Of the eight codons that contain CpG, six were markedly suppressed. The mean NNU/NNC ratio of the six amino acids using either NNC or NNU as ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.010
更新日期:2007-12-20 00:00:00
abstract::Virus genomes are condensed and packaged inside stable proteinaceous capsids that serve to protect them during transit from one cell or host organism, to the next. During virus entry, capsid shells are primed and disassembled in a complex, tightly-regulated, multi-step process termed uncoating. Here we compare the unc...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2015.01.024
更新日期:2015-05-01 00:00:00
abstract::It has previously been shown that the genome of RF virus, a variant of the human papovavirus, BK, consists of two DNA species, one (R1a) with a deletion corresponding to the early and the other (R2) with a deletion corresponding to the late region of BKV (A. Pater, M.M. Pater, and G. di Mayorca (1980). J. Virol. 36, 4...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90509-3
更新日期:1983-12-01 00:00:00
abstract::The 94-kDa virion-associated RNA-dependent RNA polymerase (RdRp) is present in infectious pancreatic necrosis virus in two forms: (i) as a free polypeptide (VP1) and (ii) as a genome linked protein (VPg) (J. G. Calvert et al., 1991, J. Gen. Virol. 72, 2563-2567). VP1 was guanylylated in vitro by incubating purified vi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1125
更新日期:1995-04-01 00:00:00
abstract::The prevalence of hepatitis B virus vaccine escape mutants has increased as a consequence of the introduction of global vaccination programs. Furthermore and as a consequence of the organization of the genome of hepatitis B virus (HBV) into overlapping reading frames, the selection of polymerase mutants during long-te...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1246
更新日期:2002-02-15 00:00:00
abstract::The heptauridine tract at each gene end and intergenic region (IGR) at the gene junctions of vesicular stomatitis virus (VSV) have effects on synthesis of the downstream mRNA, independent of their respective roles in termination of the upstream mRNA. To investigate the role of the U tract and the IGR in downstream gen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.001
更新日期:2008-10-25 00:00:00
abstract::The antibody response in serum and the reproductive tract of female rabbits to a model antigen, influenza virus haemagglutinin (HA), encoded by a recombinant myxoma virus was investigated. Strong and lasting IgG antibody responses to HA were induced in serum following intradermal, intranasal, and intravaginal immunisa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00324-6
更新日期:2003-08-15 00:00:00
abstract::The virion RNA of mouse hepatitis virus, strain A59 (MHV-A59) is believed to be the mRNA for the viral RNA-dependent RNA polymerase. The cell-free translation of virion RNA results in the synthesis of two predominant products p220 and p28 (M. R. Denison and S. Perlman, 1986, J. Virol. 60, 12-18). p28 is a basic protei...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90303-5
更新日期:1987-04-01 00:00:00
abstract::Spindle-shaped viruses are a dominant morphotype in hypersaline waters but their molecular characteristics and their relationship to other archaeal viruses have not been determined. Here, we describe the isolation, characteristics and genome sequence of His2, a spindle-shaped halovirus, and compare it to the previousl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.02.005
更新日期:2006-06-20 00:00:00
abstract::Nucleotide sequence analysis has shown previously that the genomic-length mRNA (mRNA1) of the coronavirus infectious bronchitis virus (IBV) contains two large open reading frames (ORFs), 1a and 1b, with the potential to encode polyproteins of approximately 441 and 300 kDa, respectively. We have characterized the speci...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1128
更新日期:1995-04-01 00:00:00
abstract::Cellular activation plays an important role in retroviral replication. Previously, we have shown that CD4(+)CD25(+) T cells by the virtue of their partially activated phenotype represent ideal candidates for a productive feline immunodeficiency virus (FIV) infection. In the present study, we extended our previous obse...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.02.016
更新日期:2005-05-10 00:00:00
abstract::Feline morbillivirus (FmoPV) has recently been identified in Hong Kong and Japan. FmoPV is considered to belong to the genus Morbillivirus, in the family Paramyxoviridae. In this study, the complete nucleotide sequences of three strains of FmoPV detected in cats in Japan were determined. Among the six genes in FmoPV; ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.09.003
更新日期:2014-11-01 00:00:00
abstract::Three cDNA clones that express viral gene products in Escherichia coli JM83 were derived from a watermelon mosaic virus-1 strain of papaya ringspot virus (PRSV-W). DNAs complementary to portions of the viral RNA were inserted into the pUC8 and pUC9 plasmids, and the expressed polypeptides were fusion products with the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90383-6
更新日期:1985-06-01 00:00:00
abstract::HeLa cell extracts containing wild-type copies of the minute virus of mice NS-1 polypeptide produced from a recombinant vaccinia virus vector could support the excision and replication of viral 5' telomeres from cloned concatemer junction fragments. Resolution did not occur if wild-type NS-1 was omitted from the extra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)91223-h
更新日期:1992-09-01 00:00:00
abstract::The recent development of arterivirus full-length cDNA clones makes possible the construction of chimeric arteriviruses for fundamental and applied studies. Using an equine arteritis virus (EAV) infectious cDNA clone, we have engineered chimeras in which the ectodomains of the two major envelope proteins, the glycopro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1074
更新日期:2001-09-30 00:00:00
abstract::The effect of adenovirus infection or transformation on the DNA replication of Minute Virus of Mice (MVM) was studied in human fibroblast cell lines. In WI38, HeLa, and 293 cells MVM infection allowed production of viral NS-1 and capsid proteins with or without adenovirus 2 (Ad2) co-infection. However, MVM DNA replica...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90010-o
更新日期:1990-06-01 00:00:00
abstract::Previous studies (Mertens et al., Virology 157, 375-386, 1987) have shown that removal of the outer capsid layer from bluetongue virus (BTV) significantly reduces (approximately x 10(-4)) the infectivity of the resultant core particle for mammalian cells (BHK 21 cells). In contrast, the studies reported here, using a ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0153
更新日期:1996-03-15 00:00:00
abstract::The rate of synthesis in vivo and the steady-state level of mRNA of four "model" herpes simplex virus type 1 (HSV-1) genes were measured as a function of high levels of alpha-gene products. The genes studied were ICP4 (alpha), deoxy-UTPase (beta), VP5 (beta gamma), and glycoprotein C (gC, gamma). Accumulation of high ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90318-7
更新日期:1987-03-01 00:00:00
abstract::Hepatitis C virus (HCV) core protein is a multifunctional protein interacting with cellular and viral proteins and promoters. A tetracycline-regulated system was used to generate a HepG2 Tet-Off cell line allowing regulated expression of a full-length (191 aa) and an N(c)-truncated core protein (160 aa). In this syste...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1227
更新日期:2002-01-20 00:00:00
abstract::Viruses were recovered from HeLa S3 cells and African green monkey kidney (AGMK) cells transfected with an infectious cDNA clone of poliovirus vaccine Sabin 1 strain. The viruses recovered from the different DNA-transfected cells were tested for the biological characteristics of temperature sensitivity (rct marker), p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90100-5
更新日期:1986-05-01 00:00:00
abstract::The 30K protein of tobacco mosaic virus was recently confirmed to be involved in cell-to-cell movement of the virus. To characterize common structural features of the 30K protein, the nucleotide sequence of the 30K protein gene of cucumber green mottle mosaic virus (CGMMV, a member of the tobamoviruses) RNA has been d...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00