Production and characterization of human monoclonal antibody Fab fragments to vaccinia virus from a phage-display combinatorial library.

abstract：:Epizootics of a natural varicella-like disease occur in populations of nonhuman primates. Several primate herpesviruses have been isolated from these epizootics, but the relatedness of these isolates to each other is not well-defined. In this study, we demonstrated that the restriction endonuclease (REn) profiles of f...

journal_title：Virology

authors： Gray WL,Gusick NJ

更新日期：1996-10-01 00:00:00

abstract：:Feline calicivirus depends on host-cell proteins for its replication. We previously showed that knockdown of nucleolin (NCL), a phosphoprotein involved in ribosome biogenesis, resulted in the reduction of FCV protein synthesis and virus yield. Here, we found that NCL may not be involved in FCV binding and entry into c...

journal_title：Virology

authors： Hernández BA,Sandoval-Jaime C,Sosnovtsev SV,Green KY,Gutiérrez-Escolano AL

更新日期：2016-02-01 00:00:00

abstract：:The genome of the lytic Pseudomonas putida bacteriophage gh-1 is linear double-stranded DNA containing 37,359 bp with 216-bp direct terminal repeats. Like other members of the T7 group, the gh-1 genome contains regions of high homology to T7 interspersed with nonhomologous regions that contain small open reading frame...

journal_title：Virology

authors： Kovalyova IV,Kropinski AM

更新日期：2003-07-05 00:00:00

abstract：:Previous studies with monoclonal antibodies of the antigenic structure of bovine leukemia virus (BLV) envelope glycoprotein (gp51) have identified three epitopes (F, G, H) directly involved in the infectivity of BLV, F, G, and H lost their reactivity with the respective monoclonal antibodies after treatment with a red...

journal_title：Virology

authors： Portetelle D,Couez D,Bruck C,Kettmann R,Mammerickx M,Van der Maaten M,Brasseur R,Burny A

更新日期：1989-03-01 00:00:00

abstract：:The mechanism of trans-acting regulation of transcription from the long terminal repeat (LTR) of human immunodeficiency virus (HIV) has been investigated. The roles of the cis-acting elements within HIV LTR, as well as the trans-acting factors present in HIV-infected cells, have been evaluated by an in vitro transcrip...

journal_title：Virology

authors： Okamoto T,Benter T,Josephs SF,Sadaie MR,Wong-Staal F

更新日期：1990-08-01 00:00:00

abstract：:Plant virus-encoded movement protein(s) (MP), and for many viruses the coat protein (CP), is required to mediate viral spread between plant cells via plasmodesmata (PD). Most probably, the genomic RNA of potexviruses moves through PD as assembled virions and/or as ribonucleoprotein complexes containing the CP and 25-k...

journal_title：Virology

authors： Atabekov JG,Rodionova NP,Karpova OV,Kozlovsky SV,Poljakov VY

更新日期：2000-06-05 00:00:00

abstract：:We have cloned an infectious genome of the Junonia coenia densonucleosis virus (JcDNV) into the bacterial plasmid pBR322. The viral genome could be rescued from the recombinant plasmid pBRJ by transfection of pBRJ DNA to sensitive Spodoptera littoralis larvae. pBRJ DNA produced a typical viral infection and a comparab...

journal_title：Virology

authors： Jourdan M,Jousset FX,Gervais M,Skory S,Bergoin M,Dumas B

更新日期：1990-11-01 00:00:00

abstract：:Phase I human clinical studies involving therapeutics for emerging and biodefense pathogens with low incidence, such as the severe acute respiratory syndrome coronavirus (SARS-CoV), requires at a minimum preclinical evaluation of efficacy in two well-characterized and robust animal models. Thus, a ferret SARS-CoV mode...

journal_title：Virology

authors： Chu YK,Ali GD,Jia F,Li Q,Kelvin D,Couch RC,Harrod KS,Hutt JA,Cameron C,Weiss SR,Jonsson CB

更新日期：2008-04-25 00:00:00

abstract：:The protruding (P) domain of norovirus VP1 is responsible for immune recognition and host receptor interaction. Our previous studies have demonstrated that a modification of the ends of the P domain affects the conformation and/or function of the P protein. An expression of the P domain with or without the hinge, or w...

journal_title：Virology

authors： Tan M,Fang PA,Xia M,Chachiyo T,Jiang W,Jiang X

更新日期：2011-02-20 00:00:00

abstract：:The SARS-coronavirus (SARS-CoV) is the etiological agent of the severe acute respiratory syndrome (SARS). The SARS-CoV spike (S) glycoprotein mediates membrane fusion events during virus entry and virus-induced cell-to-cell fusion. The cytoplasmic portion of the S glycoprotein contains four cysteine-rich amino acid cl...

journal_title：Virology

authors： Petit CM,Chouljenko VN,Iyer A,Colgrove R,Farzan M,Knipe DM,Kousoulas KG

更新日期：2007-04-10 00:00:00

abstract：:Tick-borne flaviviruses are maintained in nature in an enzootic cycle involving a tick vector and a vertebrate host. Thus, the virus replicates in two disparate hosts, each providing selective pressures that can influence virus replication and pathogenicity. To identify viral determinants associated with replication i...

journal_title：Virology

authors： Mitzel DN,Best SM,Masnick MF,Porcella SF,Wolfinbarger JB,Bloom ME

更新日期：2008-11-25 00:00:00

abstract：:Monoclonal antibodies (MAb) against an O1 Suisse isolate of FMDV were used to identify epitopes on the virus particle and to determine their relative function. Six major antigenic sites containing one or more epitopes were identified using competition ELISA. An epitope relationship is proposed consisting of a trypsin-...

journal_title：Virology

authors： McCullough KC,Crowther JR,Carpenter WC,Brocchi E,Capucci L,De Simone F,Xie Q,McCahon D

更新日期：1987-04-01 00:00:00

abstract：:There is growing evidence that the receptor-binding characteristics of influenza viruses are affected by the host-dependent glycosylation of viral hemagglutinin (HA). To better understand these effects, we propagated two variants of the human influenza virus USSR/90/77 (which differed by the mutation Asn131 reversible...

journal_title：Virology

authors： Gambaryan AS,Marinina VP,Tuzikov AB,Bovin NV,Rudneva IA,Sinitsyn BV,Shilov AA,Matrosovich MN

更新日期：1998-08-01 00:00:00

abstract：:Temperate coliphage P2 integrates its genome into the host chromosome upon lysogenization via a site-specific recombination event mediated by an integrase belonging to the complex family of tyrosine recombinases. The host integration site attB (BOB') is localized in the end of the cyaR gene and shares 27 nucleotides w...

journal_title：Virology

authors： Sylwan L,Frumerie C,Haggård-Ljungquist E

更新日期：2010-09-01 00:00:00

abstract：:Flock house virus (FHV) encodes a suppressor protein B2 to overcome antiviral RNA silencing during infection. Biochemical analyses have shown that a homodimer of B2 binds to double-stranded RNA to inhibit dicer-mediated cleavage of dsRNA and incorporation of small interfering RNAs into the RNA-induced silencing comple...

journal_title：Virology

authors： Seo JK,Kwon SJ,Rao AL

更新日期：2012-10-25 00:00:00

abstract：:The interferon sensitivity of the expression of an influenza-virus hemagglutinin (HA) gene cloned into the thymidine kinase (TK) gene of vaccinia virus was studied in chick embryo fibroblasts (CEF) and Madin-Darby bovine kidney (MDBK) cells. In CEF, the expression of the HA gene is inhibited by pretreatment of cells w...

journal_title：Virology

authors： Degen HJ,Blum D,Grün J,Jungwirth C

更新日期：1992-05-01 00:00:00

abstract：:The specificity of poliovirus encapsidation has been studied using a novel chimeric genome in which the gene encoding firefly luciferase has been substituted for the VP2-VP3-VP1 genes of the poliovirus capsid (P1) gene. Transfection of RNA transcribed in vitro from this genome resulted in a VP4-luciferase fusion prote...

journal_title：Virology

authors： Porter DC,Ansardi DC,Wang J,McPherson S,Moldoveanu Z,Morrow CD

更新日期：1998-03-30 00:00:00

abstract：:The causal agent of rhizomania disease, Beet necrotic yellow vein virus (BNYVV), typically produces asymptomatic root-limited infections in sugar beets (Beta vulgaris) carrying the Rz1-allele. Unfortunately, this dominant resistance has been recently overcome. Multiple cDNA clones of the viral pathogenic determinant p...

journal_title：Virology

authors： Acosta-Leal R,Fawley MW,Rush CM

更新日期：2008-06-20 00:00:00

abstract：:Mammalian type C retroviruses can be detected by their induction of foci of cell transformation in S+I - cells. We have noted that certain subtypes of the mouse xenotropic type C retrovirus (MuLV) inhibit this cell alteration. This inhibition, associated with intact virions, is irreversible and gives the infected cell...

journal_title：Virology

authors： Hoffman AD,Levy JA

更新日期：1987-09-01 00:00:00

abstract：:Mannose binding lectin (MBL) is a collagenous C-type lectin, which plays an important role in innate immunity. It can bind to carbohydrates on the surface of a wide range of pathogens, including viruses. Here we studied the antiviral effect of recombinant chicken (rc)MBL against Infectious Bronchitis Virus (IBV), a hi...

journal_title：Virology

authors： Zhang W,Bouwman KM,van Beurden SJ,Ordonez SR,van Eijk M,Haagsman HP,Verheije MH,Veldhuizen EJA

更新日期：2017-09-01 00:00:00

abstract：:The M128L myxoma virus gene expresses a five-membrane spanning cell surface protein with significant amino acid homology to the cellular CD47 proteins. CD47, also called integrin-associated protein (IAP), is associated with the modulation of leukocyte adhesion, motility, activation, and phagocytosis. Creation of an M1...

journal_title：Virology

authors： Cameron CM,Barrett JW,Mann M,Lucas A,McFadden G

更新日期：2005-06-20 00:00:00

abstract：:We have quantitated replication of the RNA genomes of vesicular stomatitis virus (VSV) and its defective interfering (DI) particles in a BHK21 cell-free system into which nucleocapsids were introduced in varying amounts and ratios, with or without addition of purified virus polymerase components. The quantitative tran...

journal_title：Virology

authors： Giachetti C,Holland JJ

更新日期：1989-05-01 00:00:00

abstract：:Transfusion of blood and blood products contaminated with the pathogenic form of prion protein Prp(sc), thought to be the causative agent of variant a Creutzfeldt-Jakob disease (vCJD), may result in serious consequences in recipients with a compromised immune system, for example, as seen in HIV-1 infection. In the pre...

journal_title：Virology

authors： Bacot SM,Feldman GM,Yamada KM,Dhawan S

更新日期：2015-02-01 00:00:00

abstract：:Adenovirus early region 1A (E1A) products are phosphorylated nuclear oncoproteins which appear to derive transforming activity largely through interactions with cellular proteins including the tumor suppressor p105/Rb-1 and cyclin A (p60cycA), a regulatory subunit associated with p34cdc2 and the related protein kinase...

journal_title：Virology

authors： Dumont DJ,Branton PE

更新日期：1992-07-01 00:00:00

abstract：:Pandemic H1N1 influenza A (H1N1pdm) elicits stronger pulmonary inflammation than previously circulating seasonal H1N1 influenza A (sH1N1), yet mechanisms of inflammatory activation in respiratory epithelial cells during H1N1pdm infection are unclear. We investigated host responses to H1N1pdm/sH1N1 infection and virus ...

journal_title：Virology

authors： Paquette SG,Banner D,Chi le TB,Leόn AJ,Xu L,Ran L,Huang SS,Farooqui A,Kelvin DJ,Kelvin AA

更新日期：2014-01-05 00:00:00

abstract：:The Epstein-Barr virus (EBV) nuclear antigen EBNA-1 is essential for viral genome maintenance in vitro and may be the only EBV protein expressed by the majority of latently infected cells in vivo. EBNA-1 may therefore be critical to the evasion of host immunity which allows persistent infection. EBNA-1 includes a poly...

journal_title：Virology

authors： Wrightham MN,Stewart JP,Janjua NJ,Pepper SD,Sample C,Rooney CM,Arrand JR

更新日期：1995-04-20 00:00:00

abstract：:Trichomonas vaginalis viruses (TVV), which may regulate P270 gene expression in the protozoan pathogen T. vaginalis, are a group of divergent double-stranded (ds) RNA viruses. In the present study, the complete 4674-bp cDNA sequence of a 4.6-kb ds RNA from a newly identified TVV2-1 isolate was determined. The sequence...

journal_title：Virology

authors： Bessarab IN,Liu HW,Ip CF,Tai JH

更新日期：2000-02-15 00:00:00

abstract：:Rift Valley fever virus-induced protein synthesis was examined by polyacrylamide gel electrophoresis and fluorography. Five virus-induced polypeptides were detected, the nucleocapsid protein N, the nucleus-associated nonstructural protein NS1, the glycoproteins G1 and G2, and a protein of molecular weight 80K. The N, ...

journal_title：Virology

authors： Struthers JK,Swanepoel R,Shepherd SP

更新日期：1984-04-15 00:00:00

abstract：:Multiplication of frog virus 3 (FV 3) occurs in mammalian cells provided they are incubated at temperatures lower than 33 degrees. The expression of the viral genome at supraoptimal temperatures was followed by analyzing the polypeptides produced in CHO-infected cells and comparing with those obtained under restrictiv...

journal_title：Virology

authors： Cordier O,Tondre L,Aubertin AM,Kirn A

更新日期：1986-07-30 00:00:00

abstract：:In this study we show that macrophages (Mphi) are latently infected with murine cytomegalovirus (MCMV). After clearance of acute MCMV infection, the predominant form of chronic infection in Balb mice is latency rather than persistence. Peritoneal exudate cells (PECs) from latently infected Balb mice (3-9 months postin...

journal_title：Virology

authors： Pollock JL,Presti RM,Paetzold S,Virgin HW 4th

更新日期：1997-01-06 00:00:00