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Terminal modifications of norovirus P domain resulted in a new type of subviral particles, the small P particles.

Abstract：

:The protruding (P) domain of norovirus VP1 is responsible for immune recognition and host receptor interaction. Our previous studies have demonstrated that a modification of the ends of the P domain affects the conformation and/or function of the P protein. An expression of the P domain with or without the hinge, or with an additional cysteine at either ends of the P protein resulted in P dimers and/or P particles. Here we report a new type of subviral particle, the small P particles, through a further modification, either an addition of the flag tag or a change of the arginine cluster, at the C-terminus of the cysteine-containing P domain. Gel filtration and cryo-EM studies showed that the small P particles are tetrahedrons formed by 6 P dimers or 12 P monomers that is half-size of the P particles. Fitting of the crystal structure of the P domain into the cryo-EM density map of the particle indicated similar conformations of the P dimers as those in P particles. The small P particles bind human HBGAs and are antigenically reactive similar to their parental VLPs and P particles. These data suggest that the C-terminus of the P domain is an important factor in the formation of the P particles. Further elucidation of the mechanism of these modifications in the P particle formation would be important in structure biology and morphogenesis of noroviruses. The small P particles may also be a useful alternative in study of norovirus-host interaction and vaccine development for noroviruses.

journal_name

Virology

journal_title

Virology

authors

Tan M,Fang PA,Xia M,Chachiyo T,Jiang W,Jiang X

doi

10.1016/j.virol.2010.11.017

subject

Has Abstract

pub_date

2011-02-20 00:00:00

pages

345-52

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(10)00735-X

journal_volume

410

pub_type

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