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Experimental validation of influenza A virus matrix protein (M1) interaction with host cellular alpha enolase and pyruvate kinase.

Abstract：

:Influenza A virus, a respiratory pathogen manipulates various host cellular processes to establish a successful infection in a host. We had reported earlier the interaction of influenza A virus nucleoprotein with host glycolytic enzymes; alpha enolase and pyruvate kinase in A549 cells. Matrix protein (M1), another multifunctional protein encoded by genome segment 7 forms the inner layer of the virion and interacts with the ribonucleoprotein complex. Nucleoprotein and matrix protein, major structural components of the virion together contribute to the stability of the capsid. Thus, we have investigated the interaction of viral matrix protein with host glycolytic enzymes; alpha enolase and pyruvate kinase. Results had demonstrated differential expression of these two glycolytic enzymes in response to matrix protein and their interaction with matrix protein by in vitro binding, co-immunoprecipitation and co-localization studies. Our results confirmed that viral matrix protein interacts with host glycolytic enzymes in association with viral nucleoprotein.

journal_name

Virology

journal_title

Virology

authors

Mishra S,Goyal P,Kumar D,Chaudhari R,Rajala MS

doi

10.1016/j.virol.2020.07.019

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

59-67

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(20)30146-X

journal_volume

549

pub_type

杂志文章

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