当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Enteric adenovirus type 40:E1B transcription map and identification of novel E1A-E1B cotranscripts in lytically infected cells.

Enteric adenovirus type 40:E1B transcription map and identification of novel E1A-E1B cotranscripts in lytically infected cells.

Abstract：

:Adenovirus 40 (Ad40) is defective for growth in tissue culture but is complemented when the Ad2/5 or Ad12 E1B 55K protein is supplied in trans. Ad40 E1B mRNA has not been detected in E1-transformed cells, or at early times in lytically infected cells. In cells constitutively expressing the E1B region of Ad2, Ad40 E1B mRNAs are detected at late times in infection, after the onset of DNA replication. We have determined the Ad40 E1B transcription map from RNA produced at late times in infected KB16 cells, using S1 nuclease, primer extension, PCR-cDNA analysis, and Northern blotting. E1B transcripts corresponding to Ad2 14 S, 22 S, and 9 S mRNAs were identified but no 13 S mRNA equivalent was detected, a pattern similar to that seen in the Ad12 transcription map. The coding potential for E1B 19K, 55K, and 15K proteins and for ppIX is retained in the Ad40 transcripts. In addition we find novel E1A-E1B cotranscript counterparts of the 14 S and 22 S mRNAs. These contain the first 40 codons of the E1A first exon linked to a site 4-5 nt downstream of the E1B cap site, retaining all the coding potential of the E1B mRNAs. No new open reading frames are created by the junction, and the E1A ORF terminates with one codon added after the junction. Each E1A-E1B cotranscript is present in abundance comparable to that of its authentic E1B counterpart. The E1A-E1B junction is unusual in that it does not conform to splice consensus sequences and thus may not be generated by a conventional splicing mechanism.

journal_name

Virology

journal_title

Virology

authors

Steinthorsdottir V,Mautner V

doi

10.1016/0042-6822(91)90478-t

subject

Has Abstract

pub_date

1991-03-01 00:00:00

pages

139-49

issue

1

eissn

0042-6822

issn

1096-0341

journal_volume

181

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Genetic variation and microevolution of dengue 2 virus in Southeast Asia.

    abstract：:Dengue 2 (DEN 2) virus strains collected from dengue hemorrhagic fever (DHF) patients and Aedes aegypti mosquitoes in Thailand, Burma, and Vietnam over a 25-year period have been analyzed by computer assisted T1-RNase-resistant oligonucleotide fingerprinting. Fifty-seven DEN 2 virus strains of the Thailand topotype we...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90195-5

    authors： Trent DW,Grant JA,Monath TP,Manske CL,Corina M,Fox GE

    更新日期：1989-10-01 00:00:00

  • Rotavirus infection of MA104 cells is inhibited by Ricinus lectin and separately expressed single binding domains.

    abstract：:Various lectins were tested for blocking rotavirus infection of MA104 cells and it was observed that galactose-specific lectins were the most inhibitory. Of these Ricinus agglutinin was able to inhibit infection (by human and animal strains) at concentrations as low as 10(-9) M. In addition, in a virus overlay protein...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0470

    authors： Jolly CL,Beisner BM,Holmes IH

    更新日期：2000-09-15 00:00:00

  • The Rice stripe virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana.

    abstract：:The Rice stripe virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis i...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.01.007

    authors： Zhang C,Pei X,Wang Z,Jia S,Guo S,Zhang Y,Li W

    更新日期：2012-04-10 00:00:00

  • Potato virus A genome-linked protein VPg is an intrinsically disordered molten globule-like protein with a hydrophobic core.

    abstract：:Genome-linked protein VPg of Potato virus A (PVA; genus Potyvirus) has essential functions in all critical steps of PVA infection, i.e. replication, movement, and virulence. Structural features of the recombinant PVA VPg were investigated with the aim to create an outline for structure-function relationships. Circular...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.04.025

    authors： Rantalainen KI,Uversky VN,Permi P,Kalkkinen N,Dunker AK,Mäkinen K

    更新日期：2008-08-01 00:00:00

  • Cellular basis of potato spindle tuber viroid systemic movement.

    abstract：:Viroids are small, nontranslatable pathogenic RNAs that replicate autonomously and traffic systemically in their host plants. We have used in situ hybridization to analyze the trafficking pattern of Potato spindle tuber viroid (PSTVd) in tomato and Nicotiana benthamiana. When PSTVd was inoculated onto the stem of a pl...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0724

    authors： Zhu Y,Green L,Woo YM,Owens R,Ding B

    更新日期：2001-01-05 00:00:00

  • Immunoprotection against influenza H5N1 virus by oral administration of enteric-coated recombinant Lactococcus lactis mini-capsules.

    abstract：:Edible vaccines that can be made widely available and easily administered could bring great benefit to the worldwide battle against pandemic viral infections. They can be used not only for the vaccination of humans and domesticated animals, but also for wild herds and live stock which are otherwise difficult to vaccin...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.08.007

    authors： Lei H,Xu Y,Chen J,Wei X,Lam DM

    更新日期：2010-11-25 00:00:00

  • Transcriptional analysis of porcine circovirus type 2.

    abstract：:Porcine circovirus type 2 (PCV2) is the causative agent of an emerging swine disease, postweaning multisystemic wasting syndrome. In this work, the RNAs of PCV2 synthesized during productive infection in porcine kidney cells were characterized. A total of nine RNAs were detected. They include the viral capsid protein ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2002.1733

    authors： Cheung AK

    更新日期：2003-01-05 00:00:00

  • Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: model systems for equine sarcoids.

    abstract：:It is now widely recognized that BPV-1 and less commonly BPV-2 are the causative agents of equine sarcoids. Here we present the generation of equine cell lines harboring BPV-1 genomes and expressing viral genes. These lines have been either explanted from sarcoid biopsies or generated in vitro by transfection of prima...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.11.037

    authors： Yuan ZQ,Gault EA,Gobeil P,Nixon C,Campo MS,Nasir L

    更新日期：2008-04-10 00:00:00

  • Carboxy-fluorescein diacetate, succinimidyl ester labeled papillomavirus virus-like particles fluoresce after internalization and interact with heparan sulfate for binding and entry.

    abstract：:Human papillomaviruses (HPVs) infect epithelial cells and are associated with genital carcinoma. Most epithelial cell lines express cell-surface glycosaminoglycans (GAGs) usually found attached to the protein core of proteoglycans. Our aim was to study how GAGs influenced HPV entry. Using a human keratinocyte cell lin...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00114-4

    authors： Drobni P,Mistry N,McMillan N,Evander M

    更新日期：2003-05-25 00:00:00

  • Influence of extended mutations of the HIV-1 transframe protein p6 on Nef-dependent viral replication and infectivity in vitro.

    abstract：:The HIV-1 transframe protein p6 known to modulate HIV-1 protease activation has been suggested to interact with the viral pathogenicity factor Nef. However, a potential interaction site in p6 has not been mapped so far. To evaluate effects of p6 modification on viral replication in light of Nef function, clustered sub...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.01.042

    authors： Leiherer A,Ludwig C,Wagner R

    更新日期：2009-04-25 00:00:00

  • Effect of expression of the aphthovirus protease 3C on viral infection and gene expression.

    abstract：:Cells transformed with specific regions of the foot-and-mouth disease virus (FMDV) genome have been constructed and analyzed with respect to viability and susceptibility to FMDV infection. Constitutive expression of an active protease 3C under the control of the tk promoter has been documented by the ability of transf...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1459

    authors： Martínez-Salas E,Domingo E

    更新日期：1995-09-10 00:00:00

  • Identification of a neutralization epitope in the VP1 capsid protein of SV40.

    abstract：:Three SV40 escape mutants were identified by selection in the presence of monoclonal antibodies with neutralizing activity. The VP1 amino acid alterations in these mutants were: (1) K73-->E (in loop BC); (2) D77-->E (in loop BC); (3) K171-->R (in loop EF); and (4) Q175-->H (in loop EF). These residues are clustered in...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.07.032

    authors： Murata H,Teferedegne B,Sheng L,Lewis AM Jr,Peden K

    更新日期：2008-11-10 00:00:00

  • Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L.

    abstract：:Priming of the viral glycoprotein (GP) by the cellular proteases cathepsin B and L (CatB, CatL) is believed to be essential for cell entry of filoviruses. However, pseudotyping systems that predominantly produce non-filamentous particles have frequently been used to prove this concept. Here, we report that GP-mediated...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.03.020

    authors： González-Hernández M,Müller A,Hoenen T,Hoffmann M,Pöhlmann S

    更新日期：2019-06-01 00:00:00

  • Cloning of SV40 genomes from human brain tumors.

    abstract：:From two human brain tumors SV40 genomes were isolated by recombinant DNA techniques. The SV40 genome cloned from a human meningioma DNA was shown to be indistinguishable from wild-type SV40. In contrast, the SV40 genome cloned from a human astrocytoma proved to be a nonviable deletion mutant with a truncated early re...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(84)90357-x

    authors： Krieg P,Scherer G

    更新日期：1984-10-30 00:00:00

  • Vaccinia virus exhibits cell-type-dependent entry characteristics.

    abstract：:Differing and sometimes conflicting data have been reported regarding several aspects of vaccinia virus (VV) entry. To address this, we used a beta-galactosidase reporter virus to monitor virus entry into multiple cell types under varying conditions. Entry into HeLa, B78H1 and L cells was strongly inhibited by heparin...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.12.029

    authors： Whitbeck JC,Foo CH,Ponce de Leon M,Eisenberg RJ,Cohen GH

    更新日期：2009-03-15 00:00:00

  • Activity of vesicular stomatitis virus M protein mutants in cell rounding is correlated with the ability to inhibit host gene expression and is not correlated with virus assembly function.

    abstract：:In addition to its role in virus assembly, the matrix (M) protein of vesicular stomatitis virus (VSV) is involved in virus-induced cell rounding and inhibition of host-directed gene expression. Previous experiments have shown that two M protein mutants genetically dissociate the ability of M protein to inhibit host-di...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.8415

    authors： Lyles DS,McKenzie MO

    更新日期：1997-03-03 00:00:00

  • Identification of the spinal cord as a major site of persistence during chronic infection with a murine coronavirus.

    abstract：:After intranasal inoculation, mouse hepatitis virus (MHV) gains entry into the central nervous system (CNS) via the olfactory and trigeminal nerves. Under the appropriate conditions, some mice develop clinically apparent demyelinating encephalomyelitis several weeks later, with virus always present in the spinal cord....

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90426-r

    authors： Perlman S,Jacobsen G,Olson AL,Afifi A

    更新日期：1990-04-01 00:00:00

  • Localization of multiple TMV encapsidation initiation sites on rbcL gene transcripts.

    abstract：:TMV capsid protein reacts with and encapsidates many of the chloroplast DNA transcripts both in vivo and in vitro to form pseudovirions. We report on the encapsidation initiation reaction with one of the major RNA species found in in vivo formed pseudovirions, the mRNA for the chloroplast-encoded large subunit of ribu...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90280-8

    authors： Atreya CD,Siegel A

    更新日期：1989-02-01 00:00:00

  • The OC43 human coronavirus envelope protein is critical for infectious virus production and propagation in neuronal cells and is a determinant of neurovirulence and CNS pathology.

    abstract：:The OC43 strain of human coronavirus (HCoV-OC43) is an ubiquitous respiratory tract pathogen possessing neurotropic capacities. Coronavirus structural envelope (E) protein possesses specific motifs involved in protein-protein interaction or in homo-oligomeric ion channel formation, which are known to play various role...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.12.023

    authors： Stodola JK,Dubois G,Le Coupanec A,Desforges M,Talbot PJ

    更新日期：2018-02-01 00:00:00

  • Characterization of the capsid associating activity of bacteriophage P4's Psu protein.

    abstract：:The Psu (Polarity suppression) protein of satellite bacteriophage P4 was first characterized as an anti-terminator of transcription termination in Escherichia coli. Psu is also a structural component of mature P4 capsids, where it is present as a decoration protein. Psu is located externally on the capsid surface, and...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1307

    authors： Isaksen ML,Dokland T,Lindqvist BH

    更新日期：1993-06-01 00:00:00

  • Transcriptional factor C/EBP binds to and transactivates the enhancer element II of the hepatitis B virus.

    abstract：:The human hepatitis B Virus genome (HBV) contains a liver-specific enhancer upstream of the X ORF which has been studied in detail by several investigators. A second liver-specific enhancer element, designated here as enhancer II, has been relatively recently described in the HBV genome, which is located within the co...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)91019-d

    authors： López-Cabrera M,Letovsky J,Hu KQ,Siddiqui A

    更新日期：1991-08-01 00:00:00

  • The HBV-producing cell line HepG2-4A5: a new in vitro system for studying the regulation of HBV replication and for screening anti-hepatitis B virus drugs.

    abstract：:All tissue culture systems for propagating HBV employed so far make use of tandemly arranged HBV genomes usually under the control of strong foreign promoters. Thus these systems are helpful for virus production but are of limited value in the investigation of the regulation of HBV replication or of the extent to whic...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0049

    authors： Weiss L,Kekulè AS,Jakubowski U,Bürgelt E,Hofschneider PH

    更新日期：1996-02-01 00:00:00

  • Morphogenesis of filamentous bacteriophage f1: orientation of extrusion and production of polyphage.

    abstract：:Crosslinking reagents were used to interrupt the process of filamentous phage morphogenesis and investigate the orientation in which nascent virions are extruded through the host cell membrane. Infected bacteria with emerging phage particles were crosslinked with glutaraldehyde. Immunoferritin-labeling studies on thes...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90382-3

    authors： Lopez J,Webster RE

    更新日期：1983-05-01 00:00:00

  • Characterization of the immediate-early functions of pseudorabies virus.

    abstract：:The immediate-early transcripts of pseudorabies virus have been located in a region of the genome situated internally within the inverted repeat between map positions 0.99 and 0.95. A single immediate-early transcript (approximately 6 kb) can be detected both in the cytoplasmic and nuclear fractions of infected, cyclo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90510-x

    authors： Ihara S,Feldman L,Watanabe S,Ben-Porat T

    更新日期：1983-12-01 00:00:00

  • A transcriptional promoter of the human parvovirus B19 active in vitro and in vivo.

    abstract：:The human parvovirus B19 causes aplastic crises in sickle cell anemia patients and the disease erythema infectiosum. So far, it has not been possible to grow B19 virus in cultured cells. Here we report the use of in vitro transcription in HeLa cell extracts and transient expression of cloned DNA transfected into HeLa ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90297-2

    authors： Doerig C,Beard P,Hirt B

    更新日期：1987-04-01 00:00:00

  • Analysis of TYA protein regions necessary for formation of the Ty1 virus-like particle structure.

    abstract：:The yeast retrotransposon, Ty1, produces a macromolecular structure known as a virus-like particle (VLP) as an essential part of its replication cycle. The Ty1 Gag-like structural protein TYA, p1-440, alone is capable of directing assembly of the VLP. In order to determine the TYA sequences required for assembly, we h...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1454

    authors： Brookman JL,Stott AJ,Cheeseman PJ,Adamson CS,Holmes D,Cole J,Burns NR

    更新日期：1995-09-10 00:00:00

  • Partial nucleotide sequence of St. Louis encephalitis virus RNA: structural proteins, NS1, ns2a, and ns2b.

    abstract：:cDNA clones of the St. Louis encephalitis (SLE) virus genome have been obtained and the nucleotide sequence of 4.7 kb corresponding to the 5' terminal half of the genome determined. The genome contains a 5' noncoding region of 98 nucleotides followed by a single continuous open reading frame that encodes three structu...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90409-0

    authors： Trent DW,Kinney RM,Johnson BJ,Vorndam AV,Grant JA,Deubel V,Rice CM,Hahn C

    更新日期：1987-02-01 00:00:00

  • Construction of chimeric arteriviruses reveals that the ectodomain of the major glycoprotein is not the main determinant of equine arteritis virus tropism in cell culture.

    abstract：:The recent development of arterivirus full-length cDNA clones makes possible the construction of chimeric arteriviruses for fundamental and applied studies. Using an equine arteritis virus (EAV) infectious cDNA clone, we have engineered chimeras in which the ectodomains of the two major envelope proteins, the glycopro...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1074

    authors： Dobbe JC,van der Meer Y,Spaan WJ,Snijder EJ

    更新日期：2001-09-30 00:00:00

  • Binding of adenovirus to its receptors in mouse astrocytes induces c-fos proto-oncogene and apoptosis.

    abstract：:We have demonstrated that Ad.betaGal, a broadly used adenoviral vector of serotype 5, binds and induces proto-oncogene c-fos expression in quiescent cultures of mouse brain astrocytes. As observed in Northern blots, the expression of this immediate early gene is induced by viral infection in a dose-dependent manner, p...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2002.1426

    authors： Rubio N,Martin-Clemente B

    更新日期：2002-06-05 00:00:00

  • Intracellular factors, but not virus receptor levels, influence the age-related outcome of DHBV infection of ducks.

    abstract：:Previous serological studies of experimental infection with duck hepatitis B virus (DHBV) have shown that the outcome of infection depends largely on the age of the duck at the time of inoculation. To examine the hypothesis that decreased susceptibility with increased age might be due to the loss of the virus receptor...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90017-j

    authors： Qiao M,Gowans EJ,Burrell CJ

    更新日期：1992-02-01 00:00:00