Abstract:
:Differing and sometimes conflicting data have been reported regarding several aspects of vaccinia virus (VV) entry. To address this, we used a beta-galactosidase reporter virus to monitor virus entry into multiple cell types under varying conditions. Entry into HeLa, B78H1 and L cells was strongly inhibited by heparin whereas entry into Vero and BSC-1 cells was unaffected. Bafilomycin also exhibited variable and cell-type-specific effects on VV entry. Entry into B78H1 and BSC-1 cells was strongly inhibited by bafilomycin whereas entry into Vero and HeLa cells was only partially inhibited suggesting the co-existence of both pH-dependent and pH-independent VV entry pathways in these cell types. Finally, entry into HeLa, B78H1, L and BSC-1 cells exhibited a lag of 6-9 min whereas this delay was undetectable in Vero cells. Our results suggest that VV exploits multiple cell attachment and entry pathways allowing it to infect a broad range of cells.
journal_name
Virologyjournal_title
Virologyauthors
Whitbeck JC,Foo CH,Ponce de Leon M,Eisenberg RJ,Cohen GHdoi
10.1016/j.virol.2008.12.029subject
Has Abstractpub_date
2009-03-15 00:00:00pages
383-91issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(08)00853-2journal_volume
385pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The primer binding site (PBS) plays pivotal roles during reverse transcription of retroviruses and also is the target of a cellular host defense impeding the transcription of murine leukemia virus (MLV) harboring a proline (pro) PBS in embryonic cells. Both the PBS and the tRNA primer are copied during reverse transcr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0683
更新日期:2000-12-20 00:00:00
abstract::Most adenovirus-specific mRNAs expressed late after infection originate from a single transcription unit, the so-called major late transcription unit. All mRNAs expressed from this unit have in common a 201 nucleotide-long spliced tripartite leader segment attached to their 5' ends. Human adenovirus mutants that carry...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1234
更新日期:1993-05-01 00:00:00
abstract::Begomoviruses (family Geminiviridae) are responsible for many economically important crop diseases worldwide. The majority of these diseases are caused by bipartite begomovirus infections, although a rapidly growing number of diseases of the Old World are associated with monopartite begomoviruses. With the exception o...
journal_title:Virology
pub_type: 杂志文章,随机对照试验,评审
doi:10.1016/j.virol.2005.09.042
更新日期:2006-01-05 00:00:00
abstract::Ultraviolet absorption spectra and difference spectra were determined for barley stripe mosaic virus (BSMV) virions and protein and for tobacco mosaic virus (TMV) virions and protein in buffer and in sodium dodecyl sulfate (SDS). The absorbance at 260 nm of BSMV virions disrupted in SDS was 92% of that of intact virio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90526-9
更新日期:1979-10-15 00:00:00
abstract::The glycosylation pattern of several flavivirus E proteins as well as the role of carbohydrate in biological functions and the antigenic structure of tick-borne encephalitis (TBE) virus were investigated by the use of specific endoglycosidases. Endoglycosidase F digestion revealed the presence of a single asparagine-l...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90460-0
更新日期:1987-08-01 00:00:00
abstract::Infectious bronchitis virus (IBV) infection and associated illness may be dramatically modified by passive transfer of immune T lymphocytes. Lymphocytes collected 10 days postinfection were transferred to naive chicks before challenge with virus. As determined by respiratory illness and viral load, transfer of syngene...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0211
更新日期:2000-03-30 00:00:00
abstract::The causal agent of rhizomania disease, Beet necrotic yellow vein virus (BNYVV), typically produces asymptomatic root-limited infections in sugar beets (Beta vulgaris) carrying the Rz1-allele. Unfortunately, this dominant resistance has been recently overcome. Multiple cDNA clones of the viral pathogenic determinant p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.008
更新日期:2008-06-20 00:00:00
abstract::Mapping of the 5' ends of virion-derived hepatitis B virus DNA molecules was carried out after cloning and sequencing linearized genomes made fully double stranded. Of five clones obtained, three of four minus strand termini mapped to the G in position 1826 and one to the T in position 1827. Plus strand 5' ends were m...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90765-h
更新日期:1992-05-01 00:00:00
abstract::The pregenomic RNA (pgRNA) of hepadnaviruses serves a dual role: as mRNA for the core (C) and polymerase (P) synthesis and as an RNA template for viral genome replication. A question arises as to how these two roles are regulated. We hypothesized that the P protein could suppress translation of the pgRNA via its inter...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.11.010
更新日期:2008-03-30 00:00:00
abstract::Epstein-Barr virus (EBV) latent membrane protein 2A (LMP2A) is expressed on the membrane of B-lymphocytes and blocks B cell receptor (BCR) signaling in EBV-transformed B-lymphocytes in vitro. The LMP2A amino-terminal domain, which is essential for the LMP2A-mediated block of B cell signal transduction, contains eight ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9964
更新日期:1999-10-25 00:00:00
abstract::A dodecapeptide containing the sequence of the C terminus of the nonstructural polyprotein of Sindbis virus has been synthesized and used to immunize rabbits. The antisera obtained precipitated polypeptides from cells infected with the HR strains of Sindbis or with temperature-sensitive mutants ts11 or ts18. Four diff...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90254-5
更新日期:1985-03-01 00:00:00
abstract::The current antiretroviral therapy (ART) can effectively reduce plasma HIV loads to undetectable levels, but cannot eliminate latently infected resting memory CD4 T cells that persist for the lifetime of infected patients. Therefore, designing new therapeutic approaches to eliminate these latently infected cells or th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.08.002
更新日期:2013-11-01 00:00:00
abstract::The presence of a genome-linked protein (VPg) at the RNA 5'-end of the genome is a characteristic of different groups of animal and plant positive-sense single-stranded RNA viruses. These viruses express their structural and functional proteins from polyproteins that are sequentially processed by at least one viral pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1165
更新日期:1994-04-01 00:00:00
abstract::Many antiviral drugs must be metabolized to their active form by cellular enzymes. Their antiviral activity may therefore be limited by an inefficient metabolism, leading to low intracellular concentration of the active form or to the accumulation of toxic intermediate metabolites. Gene transfer might be used to overc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8706
更新日期:1997-09-01 00:00:00
abstract::L83V-related variants of human papillomavirus (HPV) 16 E6, exemplified by the Asian-American variant Q14H/H78Y/L83V, were shown to be more prevalent than E6 prototype in progressing lesions and cervical cancer. We evaluated functions relevant to carcinogenesis for the E6 variants L83V, R10/L83V and Q14H/H78Y/L83V as w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.09.036
更新日期:2009-01-05 00:00:00
abstract::In vitro translation of pH 8-washed particles of tobacco mosaic virus (TMV; vulgare, common, or U1 strain) in a cell-free system derived from Escherichia coli (MRE 600) results in a broad size range of TMV RNA-encoded products, including significant amounts of the distinctive 126-kDa polypeptide which is encoded by th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90394-6
更新日期:1986-07-15 00:00:00
abstract::The nucleotide sequence of the infectious laryngotracheitis virus (ILTV) gene encoding the 205K complex glycoprotein (gp205) was determined. The gene is contained within a 3-kb EcoRI restriction fragment mapping at approximately map coordinates 0.23 to 0.25 in the UL region of the ILTV genome and is transcribed from r...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90859-a
更新日期:1991-09-01 00:00:00
abstract::When constructing viruses that have desired hybrid phenotypes, anticipated difficulties include the nonviability of many, possibly most, of the hybrid genomes that can be constructed by incorporation of DNA fragments. Therefore, many different hybrid genomes may have to be constructed in order to find one that is viab...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8328
更新日期:1997-01-20 00:00:00
abstract::We have previously demonstrated that the bZIP transcription factor CREB-2, also called ATF-4, trans-activates, in association with the viral protein Tax, the human T-cell leukemia virus type I (HTLV-I) promoter. In this study, we have examined whether CREB-2 acetylation affects transcriptional activation mediated by T...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1501
更新日期:2002-08-01 00:00:00
abstract::Pandemic H1N1 influenza A (H1N1pdm) elicits stronger pulmonary inflammation than previously circulating seasonal H1N1 influenza A (sH1N1), yet mechanisms of inflammatory activation in respiratory epithelial cells during H1N1pdm infection are unclear. We investigated host responses to H1N1pdm/sH1N1 infection and virus ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.09.022
更新日期:2014-01-05 00:00:00
abstract::MuSV349 is a TB-cell line which produces infectious MuSV with little or no MuLV detectable by the XC assay. The apparent restriction of MuLV replication in MuSV349 cells was investigated. A replication-competent helper virus, with protein composition nearly identical to that of Mo-MuLV was isolated from the viruses pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90043-6
更新日期:1984-01-30 00:00:00
abstract::CD8(+) T-lymphocytes from HIV-1 infected individuals express unidentified factors that suppress viral replication by inhibiting HIV-1 gene expression. We examined the role of epigenetics in modulating the HIV-1 suppressive factors expressed by primary CD8(+) T cells from subjects naturally controlling virus replicatio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.06.001
更新日期:2010-09-15 00:00:00
abstract::The 1.8-kbp M1 double-stranded (ds) RNA from type 1 killer strains of Saccharomyces cerevisiae contains an internal 200-bp adenine- and uracil-rich region. We have previously demonstrated that this region consists primarily of adenine residues on the plus strand of M1 dsRNA and on the full-length, in vitro synthesized...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90380-6
更新日期:1986-07-15 00:00:00
abstract::LP-BM5, a retroviral isolate, induces a disease featuring an acquired immunodeficiency syndrome termed murine AIDS (MAIDS). Many of the features of the LP-BM5-initiated disease are shared with HIV/AIDS. Our lab has shown that the interaction of B and CD4 T cells that is central to MAIDS pathogenesis requires ligation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.002
更新日期:2007-03-30 00:00:00
abstract::Piglets infected in utero with porcine reproductive and respiratory syndrome virus (PRRSV) are born severely immunocompromised. In this article we more closely examine the effects of in utero PRRSV infection on circulating and thymic T cell populations. Numbers of CD4+, CD8+, and dual-positive lymphocytes were quantit...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1650
更新日期:2002-10-25 00:00:00
abstract::To study the role of human papillomavirus (HPV) infection in the development of genetic instability, we transduced normal human airway and anogenital epithelial cells with various combinations of HPV-16 E6, E7, and the reverse transcriptase component of telomerase (hTERT). Cell lines generated by co-expression of E7 w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.06.027
更新日期:2005-09-30 00:00:00
abstract::ZEBRA, a transcription factor and DNA replication protein encoded by the Epstein-Barr virus (EBV) BZLF1 gene, plays indispensable roles in the EBV lytic cycle. We recently described the phenotypes of 46 single amino acid substitutions introduced into the DNA-recognition region of ZEBRA [Heston, L., El-Guindy, A., Coun...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.09.009
更新日期:2008-12-20 00:00:00
abstract::Transcription of human papillomaviruses (HPV) is regulated by enhancer sequences located in the upstream regulatory region. The factors regulating expression of one of the high risk genital HPV types, HPV 31b, were investigated using transient expression and protein binding assays. A region of 262 base pairs in length...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1390
更新日期:1995-08-01 00:00:00
abstract::The assembly of the tegument of herpes simplex virus type 1 (HSV-1) is a complex process that involves a number of events at various sites within virus-infected cells. Our studies focused on determining whether tegument proteins, VP1/2 and UL37, are added to capsids located within the nucleus. Capsids were isolated fr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.11.031
更新日期:2007-05-10 00:00:00
abstract::Mutants of the influenza virus A/seal/Mass/1/80 (H7N7) are described which contain an insertion of 60 nucleotides in the hemagglutinin (HA) gene, derived most probably by recombination between the HA gene and the nucleoprotein gene of the same virus. The nonhomologous RNA recombination resulted in an enhanced hemagglu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1555
更新日期:1994-10-01 00:00:00