Vaccinia virus exhibits cell-type-dependent entry characteristics.

Abstract:

:Differing and sometimes conflicting data have been reported regarding several aspects of vaccinia virus (VV) entry. To address this, we used a beta-galactosidase reporter virus to monitor virus entry into multiple cell types under varying conditions. Entry into HeLa, B78H1 and L cells was strongly inhibited by heparin whereas entry into Vero and BSC-1 cells was unaffected. Bafilomycin also exhibited variable and cell-type-specific effects on VV entry. Entry into B78H1 and BSC-1 cells was strongly inhibited by bafilomycin whereas entry into Vero and HeLa cells was only partially inhibited suggesting the co-existence of both pH-dependent and pH-independent VV entry pathways in these cell types. Finally, entry into HeLa, B78H1, L and BSC-1 cells exhibited a lag of 6-9 min whereas this delay was undetectable in Vero cells. Our results suggest that VV exploits multiple cell attachment and entry pathways allowing it to infect a broad range of cells.

journal_name

Virology

journal_title

Virology

authors

Whitbeck JC,Foo CH,Ponce de Leon M,Eisenberg RJ,Cohen GH

doi

10.1016/j.virol.2008.12.029

subject

Has Abstract

pub_date

2009-03-15 00:00:00

pages

383-91

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(08)00853-2

journal_volume

385

pub_type

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