Abstract:
:The human parvovirus B19 causes aplastic crises in sickle cell anemia patients and the disease erythema infectiosum. So far, it has not been possible to grow B19 virus in cultured cells. Here we report the use of in vitro transcription in HeLa cell extracts and transient expression of cloned DNA transfected into HeLa cells to detect and map a strong transcriptional promoter on the B19 genome. The promoter is located near the left end of the B19 genome, at position 6 map units in the clone pYT103 (approximately 280 bp upstream of the first HindIII site), and directs transcription to the right. These results suggest that the strictly limited host range of B19 does not operate at the level of transcription from the promoter at the left end of the genome.
journal_name
Virologyjournal_title
Virologyauthors
Doerig C,Beard P,Hirt Bdoi
10.1016/0042-6822(87)90297-2subject
Has Abstractpub_date
1987-04-01 00:00:00pages
539-42issue
2eissn
0042-6822issn
1096-0341journal_volume
157pub_type
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