Characterization of modified hepatitis C virus E2 proteins expressed on the cell surface.

abstract：:Pseudorabies virus (PRV) China reference strain Ea is genetically closely related to newly emerged variants; however, there is limited information about PRV Ea. Here, we compared PRV Ea with new variant strains by growth kinetics, genome sequencing, and protein expression analysis. Growth analysis showed that strain E...

journal_title：Virology

authors： Wang X,Wu CX,Song XR,Chen HC,Liu ZF

更新日期：2017-06-01 00:00:00

abstract：:The HSV-1 genome is composed of two unique regions (UL and US) flanked by inverted repeats. During the course of DNA replication the two unique regions UL and US invert relative to one another. In this report we present evidence that cleavage is not necessary for genomic inversion to occur. We isolated and characteriz...

journal_title：Virology

authors： Lamberti C,Weller SK

更新日期：1996-12-15 00:00:00

abstract：:Three different protein molecules copurify with T4 tail tubes after the tubes are released from the baseplate by guanidine hydrochloride treatment. These tube-associated proteins (TAPs) are the products of genes 29, 48, and 54. To further investigate the structural roles that these proteins may play in T4 tail assembl...

journal_title：Virology

authors： Ishimoto LK,Ishimoto KS,Cascino A,Cipollaro M,Eiserling FA

更新日期：1988-05-01 00:00:00

abstract：:Infection of adherent cell monolayers using a liquid inoculum represents an established method to reliably and quantitatively study virus infection, but poorly recapitulates the exposure and infection of cells in the respiratory tract that occurs during infection with aerosolized pathogens. To better simulate natural ...

journal_title：Virology

authors： Creager HM,Zeng H,Pulit-Penaloza JA,Maines TR,Tumpey TM,Belser JA

更新日期：2017-01-01 00:00:00

abstract：:The protein content of vaccinia virus mature virions, purified by rate zonal and isopycnic centrifugations and solubilized by SDS or a solution of urea and thiourea, was determined by the accurate mass and time tag technology which uses both tandem mass spectrometry and Fourier transform-ion cyclotron resonance mass s...

journal_title：Virology

authors： Resch W,Hixson KK,Moore RJ,Lipton MS,Moss B

更新日期：2007-02-05 00:00:00

abstract：:The identification of mechanisms that prevent infection with human immunodeficiency virus (HIV) or simian immunodeficiency virus (SIV) would facilitate the development of an effective AIDS vaccine. In time-course experiments, protection against detectable superinfection with homologous wild-type SIV was achieved withi...

journal_title：Virology

authors： Stebbings R,Berry N,Stott J,Hull R,Walker B,Lines J,Elsley W,Brown S,Wade-Evans A,Davis G,Cowie J,Sethi M,Almond N

更新日期：2004-12-05 00:00:00

abstract：:Infection with herpes simplex virus leads to amplification of SV40 DNA in various SV40-transformed cells. In earlier studies with the SV40-transformed hamster cell line Elona two different types of DNA amplification could be identified: (i) Bidirectional overreplication of chromosomally integrated SV40 DNA expanding i...

journal_title：Virology

authors： Gerspach R,Matz B

更新日期：1989-12-01 00:00:00

abstract：:The papillomavirus (PV) E2 protein activates transcription and replication by recruiting cellular proteins and the E1 DNA helicase to their binding sites in the viral genome. We recently demonstrated that phosphorylation of tyrosine 102 in the bovine papillomavirus (BPV-1) E2 protein restricts these activities and tha...

journal_title：Virology

authors： DeSmet M,Kanginakudru S,Jose L,Xie F,Gilson T,Androphy EJ

更新日期：2018-08-01 00:00:00

abstract：:The genes required for replication of the temperate bacteriophage P4, which are coded by the phage left operon, are expressed from a constitutive promoter (PLE). In the lysogenic state, repression of the P4 replication genes is achieved by premature transcription termination. The leader region of the left operon encod...

journal_title：Virology

authors： Briani F,Zangrossi S,Ghisotti D,Dehò G

更新日期：1996-09-01 00:00:00

abstract：:Magnetic birefringence measurements are reported on solutions of two double-stranded DNA containing viruses: icosahedral cauliflower mosaic virus (CaMV) and SD, an isometric bacteriophage with a short tail. Assuming the signal comes predominantly from the DNA, constraints can be put on its packing geometry. The anisot...

journal_title：Virology

authors： Torbet J,Timmins PA,Lvov Y

更新日期：1986-12-01 00:00:00

abstract：:GV1B is one of five viroids that have recently been purified from grapevines. GV1B has now been sequenced and its 363 nucleotide residues can potentially form the typical rod-like structure of viroids with 67% of nucleotides base-paired. GV1B has highest sequence similarity with grapevine yellow speckle viroid (GYSV; ...

journal_title：Virology

authors： Koltunow AM,Rezaian MA

更新日期：1989-06-01 00:00:00

abstract：:Adenovirus-mediated virotherapy is one of the promising therapeutic approaches for glioma treatment. However, its replication efficiency and specificity still failed to meet the requirements for clinical treatment. To improve the anti-tumor activity and specificity of oncolytic adenoviruses (OA), we applied multiple m...

journal_title：Virology

authors： Yao W,Guo G,Zhang Q,Fan L,Wu N,Bo Y

更新日期：2014-06-01 00:00:00

abstract：:The majority of HIV-1 infections occur via sexual intercourse. Women are the most affected by the epidemic, particularly in developing countries, due to their socio-economic dependence on men and the fact that they are often victims of gender based sexual violence. Despite significant efforts that resulted in the redu...

journal_title：Virology

authors： Alexandre KB,Mufhandu HT,London GM,Chakauya E,Khati M

更新日期：2016-10-01 00:00:00

abstract：:cDNA clones encoding the envelope glycoprotein E1 of rubella virus (RV) were altered by site-directed mutagenesis at consensus sites for addition of N-linked glycans. The resulting plasmids were introduced into COS cells and the mutant E1 proteins were analyzed by indirect immunofluorescence, radioimmunoprecipitation,...

journal_title：Virology

authors： Hobman TC,Qiu ZY,Chaye H,Gillam S

更新日期：1991-04-01 00:00:00

abstract：:The morbilliviruses are a closely related genus which are very similar in their sequences and share a common receptor, but nevertheless show significant restriction in the host species in which they cause disease. One contribution to this restriction might be the nature of the hosts' responses to infection. We have us...

journal_title：Virology

authors： Nanda SK,Baron J,Royall E,Robinson L,Falciani F,Baron MD

更新日期：2009-12-20 00:00:00

abstract：:Two forms of JC virus (JCV) have been isolated from its human host, an archetype found in kidney tissue and urine of nonimmunocompromised individuals and a rearranged type detected in lymphocytes and brain tissue of patients with and without progressive multifocal leukoencephalopathy. To investigate the hypothesis tha...

journal_title：Virology

authors： Daniel AM,Swenson JJ,Mayreddy RP,Khalili K,Frisque RJ

更新日期：1996-02-01 00:00:00

abstract：:The hepatitis B virus core open reading frame with and without the precore domain was expressed in insect cells using a baculovirus expression system. Precore antigen was not properly processed in insect cells and was present in highly insoluble cytoplasmic aggregates. Core antigen without the precore domain formed co...

journal_title：Virology

authors： Lanford RE,Notvall L

更新日期：1990-05-01 00:00:00

abstract：:Among human papillomavirus (HPV) types, clinical association with benign vs malignant lesions correlates with the ability of the corresponding oncogenes to transform cells in vitro. However, even though HPV-11 is considered a low-risk type, we have reported previously that the E5a oncogene of HPV-11gt is capable of tr...

journal_title：Virology

authors： Trujillo JM,Mounts P

更新日期：1996-06-01 00:00:00

abstract：:Human immunodeficiency virus (HIV) encodes a transacting protein, Rev, which interacts with an RNA element (RRE) to mediate nuclear export of unspliced viral mRNA. Recently, the RNA constitutive transport elements (CTE) from Mason-Pfizer monkey virus (MPMV) and simian retrovirus type I (SRV-1) were shown to render Rev...

journal_title：Virology

authors： Tang H,Xu Y,Wong-Staal F

更新日期：1997-02-17 00:00:00

abstract：:Ovine pulmonary carcinoma is a contagious neoplastic disease of sheep which can be transmitted experimentally with cell-free filtrate. Products homologous to structural proteins of type D and type B retroviruses are expressed in the tumors, but an infectious retrovirus has yet to be isolated. Retroviral RNA was isolat...

journal_title：Virology

authors： Hecht SJ,Carlson JO,DeMartin JC

更新日期：1994-07-01 00:00:00

abstract：:The differential symptom determinants of the Holmes' masked (M) and U1 strains of tobacco mosaic virus previously were mapped to the 5'-coterminal open reading frame (ORF) encoding the 126-kDa protein and the N-terminal two-thirds of the 183-kDa protein. Both proteins influence viral RNA accumulation, but the function...

journal_title：Virology

authors： Shintaku MH,Carter SA,Bao Y,Nelson RS

更新日期：1996-07-01 00:00:00

abstract：:Previous serological studies of experimental infection with duck hepatitis B virus (DHBV) have shown that the outcome of infection depends largely on the age of the duck at the time of inoculation. To examine the hypothesis that decreased susceptibility with increased age might be due to the loss of the virus receptor...

journal_title：Virology

authors： Qiao M,Gowans EJ,Burrell CJ

更新日期：1992-02-01 00:00:00

abstract：:The mitotic spindle assembly checkpoint (SAC) ensures faithful chromosome segregation during mitosis by censoring kinetochore-microtubule interactions. It is frequently rendered dysfunctional during carcinogenesis causing chromosome missegregation and genomic instability. There are conflicting reports whether the HPV1...

journal_title：Virology

authors： Yu Y,Munger K

更新日期：2012-10-10 00:00:00

abstract：:The major capsid protein of HPV, L1, assembles into pentamers that form a T = 7 icosahedral particle, but the location of the co-assembled minor capsid protein, L2, remains controversial. Several researchers have developed useful monoclonal antibodies targeting L2, but most react with linear epitopes toward the N-term...

journal_title：Virology

authors： Bywaters SM,Brendle SA,Biryukov J,Wang JW,Walston J,Milici J,Roden RB,Meyers C,Christensen ND

更新日期：2018-11-01 00:00:00

abstract：:Eight different human rotavirus strains were adapted to replicate in cell culture. In vitro, rotavirus genetic reassortment has been performed between two virus strains, one with a long pattern of segmented genomic RNAs and the other with a short pattern in the electrophoretic mobility of genomic RNAs. This genetic re...

journal_title：Virology

authors： Garbarg-Chenon A,Bricout F,Nicolas JC

更新日期：1984-12-01 00:00:00

abstract：:Rat 3Y1 cells were infected by AKR virus through microinjection of molecularly cloned proviral DNA. Based on a strong immunofluorescence using anti-p30 as an antiserum a cell clone (RESA-2) was selected that had a high expression of viral antigens. Subsequent restriction analysis of its DNA revealed that the RESA-2 cl...

journal_title：Virology

authors： van der Hoorn FA,Saris CJ,Bloemers HP

更新日期：1983-01-30 00:00:00

abstract：:Genetic variation in dengue 2 isolates from various geographic areas was examined by oligonucleotide fingerprinting of the 40 S genome RNA. Oligonucleotide maps of geographically isolated and epidemiologically unrelated viruses were very distinct. Direct comparison of the oligonucleotide map of the dengue 2 prototype ...

journal_title：Virology

authors： Trent DW,Grant JA,Rosen L,Monath TP

更新日期：1983-07-30 00:00:00

abstract：:HIV-1 variants resistant to small molecule CCR5 inhibitors such as vicriviroc (VVC) have modified Env complexes that can use both the inhibitor-bound and -free forms of the CCR5 co-receptor to enter target cells. However, entry via the inhibitor-CCR5 complex is inefficient in some, but not all, cell types, particularl...

journal_title：Virology

authors： Pugach P,Ray N,Klasse PJ,Ketas TJ,Michael E,Doms RW,Lee B,Moore JP

更新日期：2009-05-10 00:00:00

abstract：:Human embryo lung cells pretreated with a combination of human leukocyte interferon and acyclovir were infected with human cytomegalovirus (HCMV) and treated daily for 14 days with the same inhibitor combination. After removal of the inhibitors, the incubation temperature was maintained at either 37 or 40.5 degrees. I...

journal_title：Virology

authors： Bucher LJ,Wigdahl B,Rapp F

更新日期：1983-10-15 00:00:00

abstract：:The dependence of retroviral replication on cell proliferation was described as early as 1958, although different classes of retroviruses are able to infect non-dividing cells with different efficiencies. For example, the human immunodeficiency virus (HIV) and other lentiviruses infect most non-dividing cells nearly a...

journal_title：Virology

authors： Yamashita M,Emerman M

更新日期：2006-01-05 00:00:00