Abstract:
:Following a bite from an infected tick, tick-borne flaviviruses cause encephalitis, meningitis and hemorrhagic fever in humans. Although these viruses spend most of their time in the tick, little is known regarding the virus-vector interactions. We developed a simple method for synchronously infecting Ixodes scapularis larvae with Langat virus (LGTV) by immersion in media containing the virus. This technique resulted in approximately 96% of ticks becoming infected. LGTV infection and replication were demonstrated by both viral antigen expression and the accumulation of viral RNA. Furthermore, ticks transmitted LGTV to 100% of the mice and maintained the virus through molting into the next life stage. This technique circumvents limitations present in the current methods by mimicking the natural route of infection and by using attenuated virus strains to infect ticks, thereby making this technique a powerful tool to study both virus and tick determinants of replication, pathogenesis and transmission.
journal_name
Virologyjournal_title
Virologyauthors
Mitzel DN,Wolfinbarger JB,Long RD,Masnick M,Best SM,Bloom MEdoi
10.1016/j.virol.2007.03.057subject
Has Abstractpub_date
2007-09-01 00:00:00pages
410-8issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(07)00233-4journal_volume
365pub_type
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