Induction of circular episomes during rescue and replication of adeno-associated virus in experimental models of virus latency.

abstract：:To elucidate the mechanism of formation of cowpea mosaic virus (CPMV) particles, RNA-2-encoded precursor proteins were expressed in Spodoptera frugiperda cells. Processing of the 105K and 95K polyproteins in trans to give the mature Large (L) and Small (S) coat proteins required both the 32K proteinase cofactor and th...

journal_title：Virology

authors： Saunders K,Sainsbury F,Lomonossoff GP

更新日期：2009-10-25 00:00:00

abstract：:The envelope protein of yellow fever virus 17D (YFV-17D) contains a solvent-exposed RGD motif, which has led to the suggestion that integrins may function as cellular receptors for YFV-17D. We found that mutating the RGD motif to RGE had no effect on viral titers, whereas changing RGD to TGD, TGE, TAD, TAE, or RGS led...

journal_title：Virology

authors： van der Most RG,Corver J,Strauss JH

更新日期：1999-12-05 00:00:00

abstract：:Respiratory syncytial virus (RSV) is a major cause of bronchiolitis and pneumonia in infants and pneumonia virus of mice (PVM) causes similar disease. BALB/c mice are highly susceptible, while C57BL/6 mice are more resistant to PVM. IL-12 was significantly more up-regulated in response to PVM infection in BALB/c than ...

journal_title：Virology

authors： Shrivastava P,Sarkar I,Atanley E,Gomis S,van Drunen Littel-van den Hurk S

更新日期：2016-10-01 00:00:00

abstract：:The longest open reading frame of turnip yellow mosaic virus genomic RNA (ORF-206) encodes a 206-kDa nonstructural protein. The most prominent in vitro translation products of ORF-206 are the full-length p206 and a shorter N-coterminal 150-kDa protein. We have confirmed these assignments by immunoprecipitation of in v...

journal_title：Virology

authors： Bransom KL,Weiland JJ,Dreher TW

更新日期：1991-09-01 00:00:00

abstract：:Tombusviruses replicate on pre-existing organelles such as peroxisomes or mitochondria, the membranes of which become extensively reorganized into multivesicular bodies (MVBs) during the infection process. Cucumber necrosis virus (CNV) has previously been shown to replicate in association with peroxisomes in yeast. We...

journal_title：Virology

authors： Rochon D,Singh B,Reade R,Theilmann J,Ghoshal K,Alam SB,Maghodia A

更新日期：2014-03-01 00:00:00

abstract：:The P1a protein of the ipomovirus Cucumber vein yellowing virus is one of the self-cleavage serine proteases present in Potyviridae family members. P1a is located at the N-terminal end of the viral polyprotein, and is closely related to potyviral P1 protease. For its proteolytic activity, P1a requires a still unknown ...

journal_title：Virology

authors： Shan H,Pasin F,Valli A,Castillo C,Rajulu C,Carbonell A,Simón-Mateo C,García JA,Rodamilans B

更新日期：2015-02-01 00:00:00

abstract：:Natural antibodies to gp41 inhibit HIV-1 replication through the recognition of two different regions, corresponding to the leucine zipper motif in the HR1 alpha-helix and to another motif within HR2 region, hosting 2F5 and 4E10 epitope. This study aimed at reproducing such protective responses through VLP vaccination...

journal_title：Virology

authors： Pastori C,Tudor D,Diomede L,Drillet AS,Jegerlehner A,Röhn TA,Bomsel M,Lopalco L

更新日期：2012-09-15 00:00:00

abstract：:Glycoprotein B (gB, gpUL55) is a major antigen for the induction of neutralizing antibodies against human cytomegalovirus, making it an attractive antigen for active and passive immunoprophylaxis. The immunodominant region on gB is the antigenic domain 1 (AD-1), a complex structure which requires a minimal linear amin...

journal_title：Virology

authors： Schoppel K,Hassfurther E,Britt W,Ohlin M,Borrebaeck CA,Mach M

更新日期：1996-02-01 00:00:00

abstract：:Previous studies (Mertens et al., Virology 157, 375-386, 1987) have shown that removal of the outer capsid layer from bluetongue virus (BTV) significantly reduces (approximately x 10(-4)) the infectivity of the resultant core particle for mammalian cells (BHK 21 cells). In contrast, the studies reported here, using a ...

journal_title：Virology

authors： Mertens PP,Burroughs JN,Walton A,Wellby MP,Fu H,O'Hara RS,Brookes SM,Mellor PS

更新日期：1996-03-15 00:00:00

abstract：:Arabis mosaic virus is a subgroup A nepovirus. The RNA1-encoded polyprotein (P1) contains the domains for the NTP-binding protein (NTB), VPg, proteinase (Pro) and polymerase at its C-terminus. Putative cleavage sites delineating these domains have been proposed. However, the number and location of cleavage sites upstr...

journal_title：Virology

authors： Wetzel T,Chisholm J,Bassler A,Sanfaçon H

更新日期：2008-05-25 00:00:00

abstract：:Clade 2.3.4.4 H5N8 highly pathogenic avian influenza viruses (HPAIVs) have spread worldwide. Phylogenetic analysis identified two genetic groups of the H5N8 HPAIVs in South Korea; group A evolved further into four subgroups. Here, we examined the zoonotic potential, both in vivo and in vitro, of genetically distinct s...

journal_title：Virology

authors： Lee YN,Lee EK,Song BM,Heo GB,Woo SH,Cheon SH,Lee YJ

更新日期：2018-03-01 00:00:00

abstract：:The pregenomic RNA (pgRNA) of hepadnaviruses serves a dual role: as mRNA for the core (C) and polymerase (P) synthesis and as an RNA template for viral genome replication. A question arises as to how these two roles are regulated. We hypothesized that the P protein could suppress translation of the pgRNA via its inter...

journal_title：Virology

authors： Ryu DK,Kim S,Ryu WS

更新日期：2008-03-30 00:00:00

abstract：:A map of cleavage sites for the restriction endonucleases EcoRI, HindIII, BamHI, HpaI, and KpnI for a New Zealand strain of orf virus (NZ2) DNA has been deduced. Also, the entire genome, apart from approximately 0.1 kbp at each end, has been cloned into various vectors. The genome is 139 kbp in length and, in common w...

journal_title：Virology

authors： Mercer AA,Fraser K,Barns G,Robinson AJ

更新日期：1987-03-01 00:00:00

abstract：:The nucleotide sequence of the portion of naturally occurring defective EBV (HR-1) DNA (designated het DNA) which is responsible for disruption of EBV latency has been determined and compared with the regions of the standard HR-1 viral genome from which it was derived. This rearranged 2.7-kbp DNA fragment represented ...

journal_title：Virology

authors： Jenson HB,Miller G

更新日期：1988-08-01 00:00:00

abstract：:CD8+ T lymphocytes are critical for the control of gammaherpesvirus latency. To determine how memory CD8+ T cells generated during latency differ from those primed during acute or chronic viral infection, we adoptively transferred naive P14 CD8+ T cells into uninfected recipients, and examined surface proteins, cytoki...

journal_title：Virology

authors： Liu M,Barton ES,Jennings RN,Oldenburg DG,Whirry JM,White DW,Grayson JM

更新日期：2017-09-01 00:00:00

abstract：:Epstein-Barr virus (EBV) latent membrane protein 2A (LMP2A) is expressed on the membrane of B-lymphocytes and blocks B cell receptor (BCR) signaling in EBV-transformed B-lymphocytes in vitro. The LMP2A amino-terminal domain, which is essential for the LMP2A-mediated block of B cell signal transduction, contains eight ...

journal_title：Virology

authors： Swart R,Fruehling S,Longnecker R

更新日期：1999-10-25 00:00:00

abstract：:Monoclonal antibodies (MAb) against an O1 Suisse isolate of FMDV were used to identify epitopes on the virus particle and to determine their relative function. Six major antigenic sites containing one or more epitopes were identified using competition ELISA. An epitope relationship is proposed consisting of a trypsin-...

journal_title：Virology

authors： McCullough KC,Crowther JR,Carpenter WC,Brocchi E,Capucci L,De Simone F,Xie Q,McCahon D

更新日期：1987-04-01 00:00:00

abstract：:In human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) the gag gene encodes the precursor polyprotein Pr55Gag, which is cleaved by the viral protease to produce the major structural proteins. Recently, it has been shown that HIV and SIV gag RNAs contain internal ribosome entry sites (IR...

journal_title：Virology

authors： Nicholson MG,Barber SA,Clements JE

更新日期：2007-03-30 00:00:00

abstract：:When hemagglutinin (HA) and neuraminidase (NA) are presented together on an intact influenza virus particle, the antigens are competitive, with HA dominant over NA in T- and B-cell priming. Immunization with mixtures of purified HA and NA eliminates antigenic competition between HA and NA, as well as between N1-N2 NA ...

journal_title：Virology

authors： Johansson BE,Kilbourne ED

更新日期：1996-11-01 00:00:00

abstract：:The V3 loop of HIV-1 gp120 is considered occluded on many primary viruses. However, virus sensitivity to neutralization by different V3 mAbs often varies, indicating that access to V3 is not restricted equally for all antibodies. Here, we have sought to gain a better understanding of these restrictions by determining ...

journal_title：Virology

authors： Pantophlet R,Wrin T,Cavacini LA,Robinson JE,Burton DR

更新日期：2008-11-25 00:00:00

abstract：:Vaccinia virus DNA ligase has been expressed in Escherichia coli, purified, and biochemically characterized. The enzyme ligates double-stranded (ds) DNA substrates with either cohesive or blunt-end termini and the latter reaction is stimulated by PEG. Vaccinia virus DNA ligase can also ligate oligo(dT) when annealed t...

journal_title：Virology

authors： Odell M,Kerr SM,Smith GL

更新日期：1996-07-01 00:00:00

abstract：:The N-terminal domains of the NS1 protein of influenza B virus (NS1B protein) and the NS1 protein of influenza A virus (NS1A protein) share one function: binding double-stranded RNA (dsRNA). Here we show that the N-terminal domain of the NS1B protein possesses an additional function that is not shared by its NS1A coun...

journal_title：Virology

authors： Yuan W,Aramini JM,Montelione GT,Krug RM

更新日期：2002-12-20 00:00:00

abstract：:Phylogenetic analysis of the N8 neuraminidase (NA) genes from 18 influenza A viruses, representing equine and avian hosts in different geographic locations, revealed three major lineages: (i) currently circulating equine 2 viruses; (ii) avian viruses isolated in the Eurasian region, including A/Equine/Jilin/1/89, a re...

journal_title：Virology

authors： Saito T,Kawaoka Y,Webster RG

更新日期：1993-04-01 00:00:00

abstract：:To be incorporated into progeny virions, the viral genome must be transported to the inner leaflet of the plasma membrane (PM) and accumulate there. Some viruses utilize lipid components to assemble at the PM. For example, simian virus 40 (SV40) targets the ganglioside GM1 and human immunodeficiency virus type 1 (HIV-...

journal_title：Virology

authors： Kakisaka M,Yamada K,Yamaji-Hasegawa A,Kobayashi T,Aida Y

更新日期：2016-09-01 00:00:00

abstract：:Selective inactivation of critical cysteine residues in human immunodeficiency virus type one (HIV-1) was observed after treatment with 4-vinylpyridine (4-VP), with and without the membrane-permeable metal chelator N,N,N',N'-tetrakis(2-pyridylmethyl)-ethylenediamine (TPEN). Chromatographic analysis showed that cystein...

journal_title：Virology

authors： Morcock DR,Thomas JA,Sowder RC 2nd,Henderson LE,Crise BJ,Gorelick RJ

更新日期：2008-05-25 00:00:00

abstract：:Toscana (TOS) virus stocks strongly interfering with standard virus replication were obtained by sequential passages of virus in suckling mouse brain. Characterization of viral RNAs in these stocks showed the presence of a heterogeneous population of defective RNA molecules derived from the L genomic segment, in both ...

journal_title：Virology

authors： Marchi A,Nicoletti L,Accardi L,Di Bonito P,Giorgi C

更新日期：1998-06-20 00:00:00

abstract：:A cytopathic infectious agent was isolated from the kidneys of an apparently healthy tree shrew (Tupaia belangeri) that had been captured in the area around Bangkok. The infectivity was propagated in Tupaia fibroblast and kidney cell cultures. Paramyxovirus-like pleomorphic enveloped particles and helical nucleocapsid...

journal_title：Virology

authors： Tidona CA,Kurz HW,Gelderblom HR,Darai G

更新日期：1999-06-05 00:00:00

abstract：:Three different protein molecules copurify with T4 tail tubes after the tubes are released from the baseplate by guanidine hydrochloride treatment. These tube-associated proteins (TAPs) are the products of genes 29, 48, and 54. To further investigate the structural roles that these proteins may play in T4 tail assembl...

journal_title：Virology

authors： Ishimoto LK,Ishimoto KS,Cascino A,Cipollaro M,Eiserling FA

更新日期：1988-05-01 00:00:00

abstract：:While DNA vaccines have been shown to prime cellular immune responses, levels are often low in nonhuman primates or humans. Hence, efforts have been directed toward boosting responses by combining DNA with different vaccination modalities. To this end, a polyvalent DNA prime/protein boost vaccine, consisting of codon ...

journal_title：Virology

authors： Cristillo AD,Wang S,Caskey MS,Unangst T,Hocker L,He L,Hudacik L,Whitney S,Keen T,Chou TH,Shen S,Joshi S,Kalyanaraman VS,Nair B,Markham P,Lu S,Pal R

更新日期：2006-03-01 00:00:00

abstract：:Human CD4 was expressed on a range of mammalian cell lines. CD4+ non-primate cells, derived from rat, hamster, mink, cat, and rabbit, bind recombinant gp120 of human immunodeficiency virus type 1 (HIV-1) but are resistant to HIV-1 infection. CD4 expression on various human, rhesus, and African green monkey cell lines ...

journal_title：Virology

authors： Clapham PR,Blanc D,Weiss RA

更新日期：1991-04-01 00:00:00